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Development of control material for hemoglobin analysis
-
Sakorn Pornprasert
,
Kunyakan Kongthai
Published/Copyright:
November 10, 2011
Abstract
Background: There is no certified control material for hemoglobin analysis which has the hemoglobin (Hb)A2/E level as high as found in β-thalassemia trait, HbE trait, β-thalassemia/HbE disease and homozygote of HbE, the thalassemia types found frequently in the Southeast Asian population. The aim of this study was to prepare the lyophilized hemoglobin control materials for hemoglobin analysis.
Methods: Washed and dialysed erythrocytes of normal individuals and patients with β-thalassemia trait, HbE trait, β-thalassemia/HbE disease, homozygous HbE were lysed in 5% sucrose solution. The lyophilized hemoglobin control materials were prepared by using a freeze-drying (lyophilization) method. The high performance liquid chromatography (HPLC) analysis of lyophilized hemoglobin was performed after storing at –20°C for 1, 15 and 30 days and for 3 months.
Results: The chromatograms of lyophilized hemoglobin control materials showed similar patterns and similar levels of HbA, HbA2/E and HbF when compared with equivalent fresh whole blood. Moreover, the lyophilized hemoglobin presented a good correlation coefficient (r>0.990) of relationships between HPLC, low pressure liquid chromatography (LPLC) and capillary electrophoresis (CE) methods.
Conclusions: The lyophilized hemoglobin could be developed and used as control materials for hemoglobin analysis.
Keywords: capillary electrophoresis; control materials; hemoglobin analysis; high performance liquid chromatography; lyophilized hemoglobin
Received: 2011-8-6
Accepted: 2011-10-13
Published Online: 2011-11-10
Published in Print: 2012-02-01
©2012 by Walter de Gruyter Berlin Boston
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Keywords for this article
capillary electrophoresis;
control materials;
hemoglobin analysis;
high performance liquid chromatography;
lyophilized hemoglobin
Articles in the same Issue
- Editorials
- Biological variation and reference change values: an essential piece of the puzzle of laboratory testing
- Copeptin for acute coronary syndrome: diamond or decoy?
- Reviews
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- Alternative methods to a TaqMan assay to detect a tri-allelic single nucleotide polymorphism rs757210 in the HNF1β gene
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