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Lupus anticoagulant testing: analyzing fresh samples after a single centrifugation and after a 6–8 h delay
-
Paul Froom
and
Mira Barak
Published/Copyright:
October 31, 2011
Abstract
Background: For automation it is important to know the effect of analyzing fresh samples after a single rather than after two centrifugations, and to determine test stability.
Methods: This study compared silica clotting times (SCT) and dilute Russel’s viper venom tests (dRVVT) after one and two centrifugations in 50 fresh plasma samples. Then it compared test results within 4 h to those after 6–8 h of blood drawing in 40 samples. Means, minimums, maximums and quartiles of the paired screen were compared, test ratios were confirmed and correlations, linear regressions and Bland-Altman statistics were calculated.
Results: The distributions of test results were nearly identical, regardless of the number of centrifugations or timing of the analysis. The first centrifugation explained 97.7% and 94.8% of the variance of tests results after the second centrifugation for the SCT and dRVVT, respectively. The test results after 6–8 h explained 98.3% and 96.3% of the variance of the SCT-ratios and dRVVT-ratios, respectively, tested within 4 h. Inter-day coefficients of correlation of ratio comparisons were similar to those of the controls values.
Conclusions: Testing of fresh samples after a single centrifugation might replace batch testing of frozen samples after double centrifugation, providing timelier reporting of results and resulting in savings of technician time.
Keywords: centrifugation; dilute Russel’s viper venom test; fresh plasma; lupus anticoagulant; platelets; silica clotting test; stability
Received: 2011-9-20
Accepted: 2011-10-3
Published Online: 2011-10-31
Published in Print: 2012-02-01
©2012 by Walter de Gruyter Berlin Boston
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Keywords for this article
centrifugation;
dilute Russel’s viper venom test;
fresh plasma;
lupus anticoagulant;
platelets;
silica clotting test;
stability
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