Article
Licensed
Unlicensed
Requires Authentication
Evaluation of nucleated red blood cells in the peripheral blood of hematological diseases
-
Paolo Danise
,
Fabio Barrella
Published/Copyright:
October 25, 2011
Abstract
Background: Nucleated red blood cells (NRBCs) are present in the peripheral blood of several hematological and non-hematological conditions, usually associated with bad prognosis. The lack of an easy, rapid and reliable NRBCs count method did no’t allow one to know the incidence of NRBCs and to quantify them: the count was usually done during the microscopic revision of a blood smear; this is the reason we found few studies on NRBCs automated count in the literature. The aim of this study was the evaluation of the presence and the quantification of NRBCs in some onco-hematological disorders.
Methods: This study analyzed 478 patients with the automated hematology analyzer Sysmex XE2100. The range of NRBCs were calculated in the peripheral blood at diagnosis, at hematological remission and during therapy.
Results: NRBCs are present in the peripheral blood of a high number of hematological diseases and are related to ineffective erythropoiesis or stress erythropoiesis or primary alterations of hematopoiesis. NRBCs were found in nearly all onco-hematological diseases at diagnosis, but not in all patients. NRBCs were frequently found during chemotherapy and absent at remission.
Conclusions: To the authors’ knowledge, this is the first study that gives a range for NRBCs count in the peripheral blood of these diseases.
Received: 2011-8-22
Accepted: 2011-10-3
Published Online: 2011-10-25
Published in Print: 2012-02-01
©2012 by Walter de Gruyter Berlin Boston
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Editorials
- Biological variation and reference change values: an essential piece of the puzzle of laboratory testing
- Copeptin for acute coronary syndrome: diamond or decoy?
- Reviews
- Gold nanoparticles in the clinical laboratory: principles of preparation and applications
- The physiology and pathobiology of human kallikrein-related peptidase 6 (KLK6)
- Mini Review
- From “Clinical Proteomics” to “Clinical Chemistry Proteomics”: considerations using quantitative mass-spectrometry as a model approach
- Opinion Paper
- Considerations for early acute myocardial infarction rule-out for emergency department chest pain patients: the case of copeptin
- Perspectives
- Do genome-wide association scans have potential for translation?
- Genetics and Molecular Diagnostics
- Strategies of reducing input sample volume for extracting circulating cell-free nuclear DNA and mitochondrial DNA in plasma
- Evaluation of Maxwell® 16 for automated DNA extraction from whole blood and formalin-fixed paraffin embedded (FFPE) tissue
- Screening for mutations in the α-globin genes leading to abnormal hemoglobin variants with high resolution melting analysis
- Alternative methods to a TaqMan assay to detect a tri-allelic single nucleotide polymorphism rs757210 in the HNF1β gene
- Gender- and obesity-specific effect of apolipoprotein C3 gene (APOC3) –482C>T polymorphism on triglyceride concentration in Turkish adults
- Angiotensin converting enzyme (ACE) insertion/deletion (I/D) polymorphism and circulating ACE levels are not associated with outcome in critically ill septic patients
- Detection of internal tandem duplications in the FLT3 gene by different electrophoretic methods
- Functional polymorphisms of GSTA1 and GSTO2 genes associated with asthma in Italian children
- General Clinical Chemistry and Laboratory Medicine
- Optimisation of whole blood and plasma manganese assay by ICP-MS without use of a collision cell
- Quantitative performance of antibody array technology in a prenatal screening setting
- A comparison of the acute effects of calcium and strontium ranelate on the serum marker of bone resorption
- A new dot immunoassay for simultaneous detection of celiac specific antibodies and IgA-deficiency
- Preferentially immunoglobulin (IgG) subclasses production in primary Sjögren’s syndrome patients
- Development of control material for hemoglobin analysis
- Evaluation of nucleated red blood cells in the peripheral blood of hematological diseases
- The effect of hematocrit on the results of measurements using glucose meters based on different techniques
- Lupus anticoagulant testing: analyzing fresh samples after a single centrifugation and after a 6–8 h delay
- Pseudoeosinophilia of synovial fluid is caused by crystals mimicking the distinct light scattering fractions of eosinophilic granules
- The effect of acidification and oxalate concentration on urine calcium measurements in EQAS materials and patient samples
- Cancer Diagnostics
- The multi-cancer marker, rs6983267, located at region 3 of chromosome 8q24, is associated with prostate cancer in Greek patients but does not contribute to the aggressiveness of the disease
- Cardiovascular Diseases
- Heart type fatty acid binding protein in relation to pharmacologic scintigraphy in coronary artery disease
- Effects of fibrates on C-reactive protein concentrations: a meta-analysis of randomized controlled trials
- Letters to the Editor
- Comparison of Bio-Plex measurements with standard techniques
- Interference of hemoglobin (Hb) Las Palmas with HPLC measurement of HbA1c in 87 patients
- False-positive calcitonin results in patients with benign goiter
- Erratum
- Heterophilic antibody interference in commercial immunoassays; a screening study using paired native and pre-blocked sera
- Congress Abstracts
- Advances and Controversies in B-Vitamins and Choline
- Prelims
Keywords for this article
blood cell count;
hematological diseases;
nucleated red blood cells;
peripheral blood
Articles in the same Issue
- Editorials
- Biological variation and reference change values: an essential piece of the puzzle of laboratory testing
- Copeptin for acute coronary syndrome: diamond or decoy?
- Reviews
- Gold nanoparticles in the clinical laboratory: principles of preparation and applications
- The physiology and pathobiology of human kallikrein-related peptidase 6 (KLK6)
- Mini Review
- From “Clinical Proteomics” to “Clinical Chemistry Proteomics”: considerations using quantitative mass-spectrometry as a model approach
- Opinion Paper
- Considerations for early acute myocardial infarction rule-out for emergency department chest pain patients: the case of copeptin
- Perspectives
- Do genome-wide association scans have potential for translation?
- Genetics and Molecular Diagnostics
- Strategies of reducing input sample volume for extracting circulating cell-free nuclear DNA and mitochondrial DNA in plasma
- Evaluation of Maxwell® 16 for automated DNA extraction from whole blood and formalin-fixed paraffin embedded (FFPE) tissue
- Screening for mutations in the α-globin genes leading to abnormal hemoglobin variants with high resolution melting analysis
- Alternative methods to a TaqMan assay to detect a tri-allelic single nucleotide polymorphism rs757210 in the HNF1β gene
- Gender- and obesity-specific effect of apolipoprotein C3 gene (APOC3) –482C>T polymorphism on triglyceride concentration in Turkish adults
- Angiotensin converting enzyme (ACE) insertion/deletion (I/D) polymorphism and circulating ACE levels are not associated with outcome in critically ill septic patients
- Detection of internal tandem duplications in the FLT3 gene by different electrophoretic methods
- Functional polymorphisms of GSTA1 and GSTO2 genes associated with asthma in Italian children
- General Clinical Chemistry and Laboratory Medicine
- Optimisation of whole blood and plasma manganese assay by ICP-MS without use of a collision cell
- Quantitative performance of antibody array technology in a prenatal screening setting
- A comparison of the acute effects of calcium and strontium ranelate on the serum marker of bone resorption
- A new dot immunoassay for simultaneous detection of celiac specific antibodies and IgA-deficiency
- Preferentially immunoglobulin (IgG) subclasses production in primary Sjögren’s syndrome patients
- Development of control material for hemoglobin analysis
- Evaluation of nucleated red blood cells in the peripheral blood of hematological diseases
- The effect of hematocrit on the results of measurements using glucose meters based on different techniques
- Lupus anticoagulant testing: analyzing fresh samples after a single centrifugation and after a 6–8 h delay
- Pseudoeosinophilia of synovial fluid is caused by crystals mimicking the distinct light scattering fractions of eosinophilic granules
- The effect of acidification and oxalate concentration on urine calcium measurements in EQAS materials and patient samples
- Cancer Diagnostics
- The multi-cancer marker, rs6983267, located at region 3 of chromosome 8q24, is associated with prostate cancer in Greek patients but does not contribute to the aggressiveness of the disease
- Cardiovascular Diseases
- Heart type fatty acid binding protein in relation to pharmacologic scintigraphy in coronary artery disease
- Effects of fibrates on C-reactive protein concentrations: a meta-analysis of randomized controlled trials
- Letters to the Editor
- Comparison of Bio-Plex measurements with standard techniques
- Interference of hemoglobin (Hb) Las Palmas with HPLC measurement of HbA1c in 87 patients
- False-positive calcitonin results in patients with benign goiter
- Erratum
- Heterophilic antibody interference in commercial immunoassays; a screening study using paired native and pre-blocked sera
- Congress Abstracts
- Advances and Controversies in B-Vitamins and Choline
- Prelims
