Startseite Analytical performances of cystatin C turbidimetric assay: which impact on accuracy of glomerular filtration rate estimation in renal transplantation?
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Analytical performances of cystatin C turbidimetric assay: which impact on accuracy of glomerular filtration rate estimation in renal transplantation?

  • Anne-Sophie Bargnoux , Marie Perrin , Valérie Garrigue , Stéphanie Badiou , Anne-Marie Dupuy , Georges Mourad und Jean-Paul Cristol EMAIL logo
Veröffentlicht/Copyright: 26. September 2011
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Clinical Chemistry and Laboratory Medicine
Aus der Zeitschrift Clinical Chemistry and Laboratory Medicine Band 50 Heft 1

Abstract

Background: The potential use of cystatin C has recently emerged to evaluate kidney function following transplantation. Analytical performance of a particle-enhanced turbidimetric immunoassay (PETIA) using Dako Cytomation Cystatin C reagents on an Olympus AU640® analyzer was evaluated. Clinical relevance was determined by comparison with a reference method in a kidney transplant cohort.

Methods: Repeatability and reproducibility were carried out for four levels of cystatin C. Linearity was done by successive dilution of plasma samples with high level of cystatin C. Comparison study was performed against the particle-enhanced nephelemetry immunoassay (PENIA) using Siemens reagents on BNII® system. Values of the glomerular filtration rate (GFR) estimated from several predictive cystatin C- and creatinine-based equations were compared to the GFR measured by isotopic method (99mTc-DTPA). These predictive algorithms were analyzed with respect to bias, precision and accuracy.

Results: Total intra-assay and inter-assay variation coefficients were below 4% at the four levels tested. Values obtained with Dako-PETIA on AU640® were correlated with the Siemens-PENIA method (Dako-Olympus=0.88 Siemens+0.17). The cystatin C alone and the creatinine-cystatin C combined equation allowed reliable assessment of GFR in our population of renal transplants. The cystatin C-based or the combined equation moderately improved determination of GFR (about 10% compared to 175MDRD for classification into the correct K/DOQI CKD stages).

Conclusions: The use of algorithms based on cystatin C and creatinine could provide a reliable estimate of the GFR in kidney transplantation. Standardization of cystatin C assays could further improve the predicting value of cystatin C in transplantation.

Keywords: cystatin C; glomerular filtration rate; predictive equations; renal transplantation
Corresponding author: Jean-Paul Cristol, Department of Biochemistry, Lapeyronie University Hospital, 191 Avenue du Doyen Gaston Giraud, 34295 Montpellier cedex 5, France Phone: +33 467 338 314, Fax: +33 467 338 393
Received: 2011-6-27
Accepted: 2011-9-6
Published Online: 2011-09-26
Published in Print: 2012-01-01

©2011 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/cclm.2011.725
Schlagwörter für diesen Artikel
cystatin C; glomerular filtration rate; predictive equations; renal transplantation

Artikel in diesem Heft

  1. Editorial
  2. Targeted therapy for HER-2: personalized medicine for her, too
  3. Review
  4. Circulating levels of HER-2/neu oncoprotein in breast cancer
  5. Mini Review
  6. The human epidermal growth factor receptor 2 (HER2)
  7. Opinion Paper
  8. Proposed criteria for sorting examined properties in clinical laboratory reports
  9. Guidelines and Recommendations
  10. Properties and units in the clinical laboratory sciences, Part XXIII. The NPU terminology, principles and implementation –a user’s guide (Technical Report 2011) (IFCC–IUPAC)
  11. Genetics and Molecular Diagnostics
  12. Establishing high resolution melting analysis: method validation and evaluation for c-RET proto-oncogene mutation screening
  13. Decreased mRNA expression of CCL5 [RANTES] in Alzheimer's disease blood samples
  14. General Clinical Chemistry and Laboratory Medicine
  15. A risk-analysis approach to the evaluation of analytical quality
  16. C-statistics versus logistic regression for assessing the performance of qualitative diagnostic tests
  17. Detection and quantification of M-proteinemia: comparison of various methods for serum protein electrophoresis
  18. External quality assessment of point-of-care International Normalized Ratio (INR) testing in Europe
  19. Comparison of two point-of-care testing (POCT) devices for fetal lactate during labor
  20. Simultaneous quantification of 19 drugs/metabolites in urine important for pain management by liquid chromatography-tandem mass spectrometry
  21. Enzyme-linked immunoassay for plasma-free metanephrines in the biochemical diagnosis of phaeochromocytoma in adults is not ideal
  22. The old and new tests for celiac disease: which is the best test combination to diagnose celiac disease in pediatric patients?
  23. Improving diagnosis of adult-type hypolactasia in patients with abdominal complaints
  24. Synovial and serum levels of uncarboxylated matrix Gla-protein (ucMGP) in patients with arthritis
  25. The diagnostic performance of allergen-molecules in comparison to allergen-extracts
  26. Analytical performances of cystatin C turbidimetric assay: which impact on accuracy of glomerular filtration rate estimation in renal transplantation?
  27. Evaluation of Aution Max AX-4030 and 9UB Uriflet, 10PA Aution Sticks urine dipsticks in the automated urine test strip analysis
  28. Reference Values and Biological Variations
  29. Diurnal variability of total calcium during normal sleep and after an acute shift of sleep
  30. Infections Diseases
  31. Comparison of two immunoassays for determining hepatitis B virus serum markers
  32. Cardiovascular Diseases
  33. Ultra-sensitive troponin I levels to exclude acute myocardial infarction from myocardial injury
  34. Diabetes
  35. Extracellular matrix-associated (GAGs, CTGF), angiogenic (VEGF) and inflammatory factors (MCP-1, CD40, IFN-γ) in type 1 diabetes mellitus nephropathy
  36. Letters to the Editor
  37. Serum HER-2/ECD analysis in monitoring breast cancer patients
  38. No evidence for the involvement of the lipoxin A4 receptor (FPR2/ALX) gene in the susceptibility to coronary artery disease
  39. Does the presence of 3-epi-25OHD3 affect the routine measurement of vitamin D using liquid chromatography tandem mass spectrometry?
  40. Validity of the measurement of capillary cholesterol in the diagnosis of defined hypercholesterolemia
  41. Prelims
  42. Prelims
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