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Vitamin D metabolism in living kidney donors before and after organ donation

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Published/Copyright: May 9, 2022
Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 60 Issue 8

Abstract

Objectives

Living kidney donors provide a unique setting to study functional and metabolic consequences after organ donation. Since the lack of data of the homoeostasis of numerous vitamin D metabolites in these healthy subjects, the aim of this study was to assess the vitamin D metabolism before and after kidney donation.

Methods

We investigated the 25-dihydroxyvitamin D2 (25[OH]D2), 25-dihydroxyvitamin D3 (25[OH]D3), 1,25-dihydroxyvitamin D3 (1,25[OH]2D3), 24,25-dihydroxyvitamin D3 (24,25[OH]2D3), 25,26-dihydroxyvitamin D3 (25,26[OH]2D3), and the native vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol) in a well characterized study cohort of 32 healthy living kidney donors before and after organ donation.

Results

Thirty-two healthy subjects after kidney donation had significantly lower median (interquartile range) 1,25(OH)2D3 serum concentrations (88.6 [62.6–118.8] vs. 138.0 [102.6–152.4] pmol/L, p<0.001) and significantly higher median 25(OH)D2 serum levels (1.80 [1.19–2.19] vs. 1.11 [0.74–1.59] nmol/L, p=0.019) than before donation. Similar serum concentrations of 25(OH)D3 and 25,26(OH)2D3 were observed before and after donation. The 24,25(OH)2D3 blood levels distinctly decreased after organ donation (4.1 [2.3–5.3] vs. 5.3 [2.2–6.9] nmol/L, p=0.153). Native vitamin D2 (0.10 [0.08–0.14] vs. 0.08 [0.06–0.12] nmol/L, p=0.275) was slightly increased and vitamin D3 (1.6 [0.6–7.2] vs. 2.5 [0.9–8.6] nmol/L, p=0.957) decreased after kidney donation.

Conclusions

Living kidney donors were found with decreased 1,25(OH)2D3 and 24,25(OH)2D3, increased 25(OH)D2 and consistent 25(OH)D3 and 25,26(OH)2D3 serum concentrations after organ donation. The current study advances the understanding on vitamin D metabolism suggesting that altered hydroxylase-activities after donation is accompanied by compensatory elevated dietary-related 25(OH)D2 blood concentrations.

Keywords: living kidney donors; metabolism; nephrectomy; vitamin D
Corresponding author: Andreas Meinitzer, Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Auenbruggerplatz 15, 8036 Graz, Austria, Phone: +43 316 385 145, Fax: +43 316 385 13430, E-mail:

  1. Research funding: None declared.

  2. Author contributions: All listed authors contributed to the study design. D.E. wrote the manuscript and S. Zitta supervised the study. S. Zitta, K.A., A.R.R., S. Zelzer and A.M. collected the data. D.E., A.M., and S. Zelzer analysed and interpreted the data. All authors provided critical feedback and finally approved the version to be published.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: The ethical approval was provided by the Ethical Committee of the Medial University of Graz (Graz, Austria) (approval number: 31-289 ex 18/19). The study was carried out in accordance with the Declaration of Helsinki.

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Received: 2022-02-17
Accepted: 2022-04-29
Published Online: 2022-05-09
Published in Print: 2022-07-26

© 2022 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/cclm-2022-0148
Keywords for this article
living kidney donors; metabolism; nephrectomy; vitamin D

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. Transdermal measurement of cardiac troponins: the future is now
  4. Reviews
  5. Perinatal presepsin assessment: a new sepsis diagnostic tool?
  6. Hypertriglyceridemia, a causal risk factor for atherosclerosis, and its laboratory assessment
  7. Opinion Paper
  8. The novelties of the regulation on health technology assessment, a key achievement for the European union health policies
  9. General Clinical Chemistry and Laboratory Medicine
  10. Performance of four regression frameworks with varying precision profiles in simulated reference material commutability assessment
  11. Comparison of six regression-based lot-to-lot verification approaches
  12. Failure Mode and Effects Analysis (FMEA) at the preanalytical phase for POCT blood gas analysis: proposal for a shared proactive risk analysis model
  13. Evaluation of a pneumatic tube system carrier prototype with fixing mechanism allowing for automated unloading
  14. Analytical performance of eight enzymatic assays for ethanol in serum evaluated by data from the Belgian external quality assessment scheme
  15. Vitamin D metabolism in living kidney donors before and after organ donation
  16. Validation of steroid ratios for random urine by mass spectrometry to detect 5α-reductase deficiency in Vietnamese children
  17. Evaluation of serum neurofilament light in the early management of mTBI patients
  18. Assessment of urine sample quality by the simultaneous measurement of urinary γ-glutamyltransferase and lactate dehydrogenase enzyme activities: possible application to unravel cheating in drugs of abuse testing
  19. Reference Values and Biological Variations
  20. Age and sex specific reference intervals of 13 hematological analytes in Chinese children and adolescents aged from 28 days up to 20 years: the PRINCE study
  21. Cancer Diagnostics
  22. Prostate health index (PHI) as a reliable biomarker for prostate cancer: a systematic review and meta-analysis
  23. A comparison of the faecal haemoglobin concentrations and diagnostic accuracy in patients suspected with colorectal cancer and serious bowel disease as reported on four different faecal immunochemical test systems
  24. Circulating cell-free DNA undergoes significant decline in yield after prolonged storage time in both plasma and purified form
  25. Cardiovascular Diseases
  26. Analytical and clinical performance evaluation of a new high-sensitivity cardiac troponin I assay
  27. Infectious Diseases
  28. Results of a SARS-CoV-2 virus genome detection external quality assessment round focusing on sensitivity of assays and pooling of samples
  29. Letters to the Editors
  30. Improving D-dimer testing appropriateness by controlling periodicity of retesting: prevention is better than cure
  31. Biological variation of serum cholinesterase catalytic concentrations
  32. Three-month ad interim analysis of total anti-SARS-CoV-2 antibodies in healthy recipient of a single BNT162b2 vaccine booster
  33. Fibrin strands in peripheral blood smear: the COVID-19 era
  34. Fragments of alpha-1-antitrypsin in patients with severe COVID-19 and bacterial pulmonary sepsis
  35. Comparison of thyroid stimulating hormone, free thyroxine, total triiodothyronine, thyroglobulin and peroxidase antibodies measurements by two different platforms
  36. Effect of different incubation times on the detection of factor VIII inhibitor in acquired hemophilia A
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