Startseite Introduction of BD Vacutainer® Barricor™ tubes in clinical biobanking and application of amino acid and cytokine quality indicators to Barricor plasma
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Introduction of BD Vacutainer® Barricor™ tubes in clinical biobanking and application of amino acid and cytokine quality indicators to Barricor plasma

  • Nadine Knutti EMAIL logo , Sophie Neugebauer , Franziska Scherr , Conny Mathay , Monica Marchese , Estelle Henry , Julia Palm , Fay Betsou und Michael Kiehntopf
Veröffentlicht/Copyright: 21. Januar 2022
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Clinical Chemistry and Laboratory Medicine (CCLM)
Aus der Zeitschrift Clinical Chemistry and Laboratory Medicine (CCLM) Band 60 Heft 5

Abstract

Objectives

The use of BD Vacutainer® Barricor™ tubes (BAR) can reduce turnaround time (TAT) and improve separation of plasma from cellular components using a specific mechanical separator. Concentrations of amino acids (AAs) and cytokines, known to be labile during pre-analytical time delays, were compared in heparin (BAR, BD Heparin standard tube [PST]), EDTA and serum gel tubes (SER) to validate previously identified quality indicators (QIs) in BAR.

Methods

Samples of healthy individuals (n=10) were collected in heparin, EDTA and SER tubes and exposed to varying pre- and post-centrifugation delays at room temperature (RT). Cytokines (interleukin [IL]-8, IL-16 and sCD40L) were analyzed by enzyme-linked immunosorbent assay (ELISA) and AAs were characterized by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS).

Results

All QIs, AAs/AA ratio and cytokines increased during prolonged blood storage in heparin plasma (PST, BAR) and SER tubes. Comparison of 53 h/1 h pre-centrifugation delay resulted in an increase in taurine (Tau) and glutamic acid (Glu) concentrations by more than three times, soluble CD40L increased by 13.6, 9.2 and 4.3 fold in PST, BAR-CTRL and BAR-FAST, and IL-8 increased even more by 112.8 (PST), 266.1 (BAR-CTRL), 268.1 (BAR-FAST) and 70.0 (SER) fold, respectively. Overall, compared to prolonged blood storage, effects of post-centrifugation delays were less pronounced in all tested materials.

Conclusions

BAR tubes are compatible with the use of several established QIs and can therefore be used in clinical biobanking to reduce pre-analytical TAT without compromising QIs and thus pre-analytical sample quality analysis.

Keywords: BD Vacutainer Barricor™ (BAR); biobanking; centrifugation; heparin-plasma; pre-analytical time delay; quality indicator (QI)
Corresponding author: Dr. Nadine Knutti, Institute of Clinical Chemistry and Laboratory Diagnostics, Jena University Hospital, Am Klinikum 1, 07747 Jena, Germany, Phone: +49 (3641) 9 325147, Fax: +49 (3641) 9 325012, E-mail:

Funding source: BMBF

Award Identifier / Grant number: 01EK1505B

Acknowledgments

We thank Kerstin Stein for her support in sample collection and AA analysis at the Institute of Clinical Chemistry and Laboratory Diagnostics and the Integrated Biobank in Jena (IBBJ) for excellent technical assistance in sample collection, transport and storage.

  1. Research funding: The study was funded by the BMBF (01EK1505B). The funding organization played no role in the study design, collection, analysis and interpretation of data, in the writing of the article or in the decision to submit the article for publication.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: Human sample collections of the study followed all relevant national regulations, institutional policies and have been approved by the Ethics Committee of the University Hospital Jena and the CNER, Comité National d’Ethique de Recherche Luxembourg.

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/cclm-2021-0899).

Received: 2021-08-12
Accepted: 2022-01-10
Published Online: 2022-01-21
Published in Print: 2022-04-26

© 2022 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Editorial
  3. Lot-to-lot variation: no longer a neglected issue
  4. Review
  5. Health Technology Assessment to assess value of biomarkers in the decision-making process
  6. Mini Review
  7. Diagnostic performance of the fully automated Roche Elecsys SARS-CoV-2 antigen electrochemiluminescence immunoassay: a pooled analysis
  8. Opinion Papers
  9. Preanalytical quality improvement – an interdisciplinary journey
  10. Metrological traceability and clinical traceability of laboratory results – the role of commutability in External Quality Assurance
  11. Lot-to-lot reagent verification: challenges and possible solutions
  12. EFLM Paper
  13. An approach for determining allowable between reagent lot variation
  14. General Clinical Chemistry and Laboratory Medicine
  15. Introduction of BD Vacutainer® Barricor™ tubes in clinical biobanking and application of amino acid and cytokine quality indicators to Barricor plasma
  16. Delays during PBMC isolation have a moderate effect on yield, but severly compromise cell viability
  17. Investigation of the effects of pneumatic tube transport system on routine biochemistry, hematology, and coagulation tests in Ankara City Hospital
  18. Comparison of three different protocols for obtaining hemolysis
  19. Report from the HarmoSter study: impact of calibration on comparability of LC-MS/MS measurement of circulating cortisol, 17OH-progesterone and aldosterone
  20. Point-of-care testing in primary healthcare: a scoring system to determine the frequency of performing internal quality control
  21. Hematology and Coagulation
  22. The flagging features of the Sysmex XN-10 analyser for detecting platelet clumps and the impacts of platelet clumps on complete blood count parameters
  23. Cancer Diagnostics
  24. Thyroglobulin and thyroglobulin antibodies: assay-dependent management consequences in patients with differentiated thyroid carcinoma
  25. Infectious Diseases
  26. Hyris bCUBE SARS-CoV-2 rapid molecular saliva testing: a POCT innovation on its way
  27. Ultrasensitive assay for saliva-based SARS-CoV-2 antigen detection
  28. Identification of contagious SARS-CoV-2 infected individuals by Roche’s Rapid Antigen Test
  29. Monocyte distribution width (MDW) as a screening tool for early detecting sepsis: a systematic review and meta-analysis
  30. Perinatal asphyxia partly affects presepsin urine levels in non-infected term infants
  31. Letters to the Editors
  32. Spurious results for total and free prostate-specific antigen (PSA); sometimes really “a riddle wrapped in a mystery inside an enigma”
  33. Reply to: Spurious results for total and free prostate-specific antigen (PSA); sometimes really “a riddle wrapped in a mystery inside an enigma”
  34. Spike vs. nucleocapsid serum antigens for COVID-19 diagnosis and severity assessment
  35. A rapid semi-quantitative test for determination of SARS-CoV-2 antibody levels
  36. Diagnostic potential of leukocyte differential and cell population data in prediction of COVID-19 among related viral and bacterial infections at Emergency Department
  37. Added value of drug-laboratory test interaction alerts in test result authorisation
  38. Pre-analytical considerations for the analysis of uracil and 5,6-dihydrouracil in heparin plasma
  39. Rare unstable and low oxygen affinity haemoglobin variant, Hb Hazebrouck, detected on Sysmex XN-9000
  40. Multicancer early detection
https://doi.org/10.1515/cclm-2021-0899
Schlagwörter für diesen Artikel
BD Vacutainer Barricor™ (BAR); biobanking; centrifugation; heparin-plasma; pre-analytical time delay; quality indicator (QI)

Artikel in diesem Heft

  1. Frontmatter
  2. Editorial
  3. Lot-to-lot variation: no longer a neglected issue
  4. Review
  5. Health Technology Assessment to assess value of biomarkers in the decision-making process
  6. Mini Review
  7. Diagnostic performance of the fully automated Roche Elecsys SARS-CoV-2 antigen electrochemiluminescence immunoassay: a pooled analysis
  8. Opinion Papers
  9. Preanalytical quality improvement – an interdisciplinary journey
  10. Metrological traceability and clinical traceability of laboratory results – the role of commutability in External Quality Assurance
  11. Lot-to-lot reagent verification: challenges and possible solutions
  12. EFLM Paper
  13. An approach for determining allowable between reagent lot variation
  14. General Clinical Chemistry and Laboratory Medicine
  15. Introduction of BD Vacutainer® Barricor™ tubes in clinical biobanking and application of amino acid and cytokine quality indicators to Barricor plasma
  16. Delays during PBMC isolation have a moderate effect on yield, but severly compromise cell viability
  17. Investigation of the effects of pneumatic tube transport system on routine biochemistry, hematology, and coagulation tests in Ankara City Hospital
  18. Comparison of three different protocols for obtaining hemolysis
  19. Report from the HarmoSter study: impact of calibration on comparability of LC-MS/MS measurement of circulating cortisol, 17OH-progesterone and aldosterone
  20. Point-of-care testing in primary healthcare: a scoring system to determine the frequency of performing internal quality control
  21. Hematology and Coagulation
  22. The flagging features of the Sysmex XN-10 analyser for detecting platelet clumps and the impacts of platelet clumps on complete blood count parameters
  23. Cancer Diagnostics
  24. Thyroglobulin and thyroglobulin antibodies: assay-dependent management consequences in patients with differentiated thyroid carcinoma
  25. Infectious Diseases
  26. Hyris bCUBE SARS-CoV-2 rapid molecular saliva testing: a POCT innovation on its way
  27. Ultrasensitive assay for saliva-based SARS-CoV-2 antigen detection
  28. Identification of contagious SARS-CoV-2 infected individuals by Roche’s Rapid Antigen Test
  29. Monocyte distribution width (MDW) as a screening tool for early detecting sepsis: a systematic review and meta-analysis
  30. Perinatal asphyxia partly affects presepsin urine levels in non-infected term infants
  31. Letters to the Editors
  32. Spurious results for total and free prostate-specific antigen (PSA); sometimes really “a riddle wrapped in a mystery inside an enigma”
  33. Reply to: Spurious results for total and free prostate-specific antigen (PSA); sometimes really “a riddle wrapped in a mystery inside an enigma”
  34. Spike vs. nucleocapsid serum antigens for COVID-19 diagnosis and severity assessment
  35. A rapid semi-quantitative test for determination of SARS-CoV-2 antibody levels
  36. Diagnostic potential of leukocyte differential and cell population data in prediction of COVID-19 among related viral and bacterial infections at Emergency Department
  37. Added value of drug-laboratory test interaction alerts in test result authorisation
  38. Pre-analytical considerations for the analysis of uracil and 5,6-dihydrouracil in heparin plasma
  39. Rare unstable and low oxygen affinity haemoglobin variant, Hb Hazebrouck, detected on Sysmex XN-9000
  40. Multicancer early detection
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