Renal tubular epithelial cells add value in the diagnosis of upper urinary tract pathology
-
Matthijs Oyaert
,
Marijn Speeckaert
Abstract
Background
Diagnosis of upper urinary tract infections (UTI) is challenging. We evaluated the analytical and diagnostic performance characteristics of renal tubular epithelial cells (RTECs) and transitional epithelial cells (TECs) on the Sysmex UF-5000 urine sediment analyzer.
Methods
Urinary samples from 506 patients presenting with symptoms of a UTI were collected. Only samples for which a urinary culture was available were included. Analytical (imprecision, accuracy, stability and correlation with manual microscopy) and diagnostic performance (sensitivity and specificity) were evaluated.
Results
The Sysmex UF-5000 demonstrated a good analytical performance. Depending on the storage time, storage conditions (2–8 °C or 20–25 °C) and urinary pH, RTECs and TECs were stable in urine for at least 4 h. Using Passing-Bablok and Bland-Altman analysis, an acceptable agreement was observed between the manual and automated methods. Compared to TECs, RTECs demonstrated an acceptable diagnostic performance for the diagnosis of upper UTI.
Conclusions
While TECs do not seem to serve as a helpful marker, increased urinary levels of RTECs add value in the diagnosis of upper UTI and may be helpful in the discrimination between upper and lower UTIs.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2020 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Editorial
- New insights on the analytical performances for detecting and quantifying monoclonal proteins
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- Early predictors of perinatal brain damage: the role of neurobiomarkers
- Opinion Paper – Point
- Mixing studies for lupus anticoagulant: mostly yes, sometimes no
- Opinion Paper – Counterpoint
- Mixing studies for lupus anticoagulant: mostly no, sometimes yes
- EFLM Consensus Paper
- Quantifying atherogenic lipoproteins for lipid-lowering strategies: consensus-based recommendations from EAS and EFLM
- Guidelines and Recommendations
- PREDICT: a checklist for preventing preanalytical diagnostic errors in clinical trials
- Genetics and Molecular Diagnostics
- Plasma vs. serum in circulating tumor DNA measurement: characterization by DNA fragment sizing and digital droplet polymerase chain reaction
- General Clinical Chemistry and Laboratory Medicine
- An international multi-center serum protein electrophoresis accuracy and M-protein isotyping study. Part I: factors impacting limit of quantitation of serum protein electrophoresis
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- National survey on delta checks in clinical laboratories in China
- Assessment of the glomerular filtration rate (GFR) in kidney transplant recipients using Bayesian estimation of the iohexol clearance
- Performance of Afinion HbA1c measurements in general practice as judged by external quality assurance data
- Renal tubular epithelial cells add value in the diagnosis of upper urinary tract pathology
- Reference Values and Biological Variations
- Influence of ethnicity on biochemical markers of health and disease in the CALIPER cohort of healthy children and adolescents
- Cardiovascular Diseases
- Clinical evaluation of capillary B-type natriuretic peptide testing
- Infectious Diseases
- Thrombo-inflammatory prognostic score improves qSOFA for risk stratification in patients with sepsis: a retrospective cohort study
- Corrigendum
- Corrigendum to: Pediatric reference intervals for 29 Ortho VITROS 5600 immunoassays using the CALIPER cohort of healthy children and adolescents
- Letters to the Editor
- Potential risks in fecal microbiota transplantation
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- Factitious severe acidosis in a patient, preanalytical considerations and prevention
- Methods for quick, accurate and cost-effective determination of the type 1 diabetes genetic risk score (T1D-GRS)
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Artikel in diesem Heft
- Frontmatter
- Editorial
- New insights on the analytical performances for detecting and quantifying monoclonal proteins
- Reviews
- Vitamin C measurement in critical illness: challenges, methodologies and quality improvements
- Early predictors of perinatal brain damage: the role of neurobiomarkers
- Opinion Paper – Point
- Mixing studies for lupus anticoagulant: mostly yes, sometimes no
- Opinion Paper – Counterpoint
- Mixing studies for lupus anticoagulant: mostly no, sometimes yes
- EFLM Consensus Paper
- Quantifying atherogenic lipoproteins for lipid-lowering strategies: consensus-based recommendations from EAS and EFLM
- Guidelines and Recommendations
- PREDICT: a checklist for preventing preanalytical diagnostic errors in clinical trials
- Genetics and Molecular Diagnostics
- Plasma vs. serum in circulating tumor DNA measurement: characterization by DNA fragment sizing and digital droplet polymerase chain reaction
- General Clinical Chemistry and Laboratory Medicine
- An international multi-center serum protein electrophoresis accuracy and M-protein isotyping study. Part I: factors impacting limit of quantitation of serum protein electrophoresis
- An international multi-center serum protein electrophoresis accuracy and M-protein isotyping study. Part II: limit of detection and follow-up of patients with small M-proteins
- Use of clinical data and acceleration profiles to validate pneumatic transportation systems
- National survey on delta checks in clinical laboratories in China
- Assessment of the glomerular filtration rate (GFR) in kidney transplant recipients using Bayesian estimation of the iohexol clearance
- Performance of Afinion HbA1c measurements in general practice as judged by external quality assurance data
- Renal tubular epithelial cells add value in the diagnosis of upper urinary tract pathology
- Reference Values and Biological Variations
- Influence of ethnicity on biochemical markers of health and disease in the CALIPER cohort of healthy children and adolescents
- Cardiovascular Diseases
- Clinical evaluation of capillary B-type natriuretic peptide testing
- Infectious Diseases
- Thrombo-inflammatory prognostic score improves qSOFA for risk stratification in patients with sepsis: a retrospective cohort study
- Corrigendum
- Corrigendum to: Pediatric reference intervals for 29 Ortho VITROS 5600 immunoassays using the CALIPER cohort of healthy children and adolescents
- Letters to the Editor
- Potential risks in fecal microbiota transplantation
- Macro-aspartate aminotransferase syndrome: a case report
- Factitious severe acidosis in a patient, preanalytical considerations and prevention
- Methods for quick, accurate and cost-effective determination of the type 1 diabetes genetic risk score (T1D-GRS)
- Anti-adalimumab and anti-certolizumab antibodies titers after discontinuation of adalimumab: two case reports
- Serum immunoglobulin-A (IgA) concentrations in a general adult population: association with demographics and prevalence of selective IgA deficiency
- Evaluation of PFA-100 closure times in cord blood samples of healthy term and preterm neonates
- Disagreement between direct and indirect potentiometric Na+ determination in infancy and childhood
- Carryover issues with UF-5000 urine flow cytometry – how did we miss it?
