Influence of ethnicity on biochemical markers of health and disease in the CALIPER cohort of healthy children and adolescents
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Houman Tahmasebi
Abstract
Background
Accurate pediatric reference intervals (RIs) for laboratory tests determined in a healthy pediatric population are essential for correct laboratory test interpretation and clinical decision-making. In pediatrics, RIs require partitioning by age and/or sex; however, the need for partitioning based on ethnicity is unclear. Here, we assessed the influence of ethnicity on biomarker concentrations in the Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) cohort of healthy children and adolescents and compared the results with the National Health and Nutrition Examination Survey (NHANES).
Methods
A total of 52 biomarkers were measured in a multiethnic population of 846–1179 healthy children (aged 5 to <19 years) upon informed consent. Biomarker concentrations were retrospectively compared between four major ethnic groups (i.e. Black, Caucasian, East Asian, and South Asian, determined by parental ethnicity). Retrospective results were verified prospectively using an additional 500 healthy pediatric samples with equal sample size across ethnicities. Ethnic-specific differences were assessed based on statistical significance and biological and analytical variations. Appropriate age-, sex-, and ethnic-specific RIs were calculated.
Results
Ethnic-specific differences were not observed for 34 biomarkers examined in the retrospective analysis, while 18 demonstrated statistically significant ethnic differences. Among these, seven analytes demonstrated ethnic-specific differences in the prospective analysis: vitamin D, amylase, ferritin, follicle-stimulating hormone (FSH), immunoglobulin A (IgA), immunoglobulin G (IgG), and immunoglobulin M (IgM). Analysis of select NHANES data confirmed CALIPER findings.
Conclusions
This is the first comprehensive Canadian pediatric study examining ethnic-specific differences in common biomarkers. While the majority of biomarkers did not require ethnic partitioning, ethnic-specific RIs were established for seven biomarkers showing marked differences. Further studies in other populations are needed to confirm our findings.
Funding source: Canadian Institutes of Health Research
Award Identifier / Grant number: 353989
Funding statement: This research was supported by the Canadian Institutes of Health Research (funder Id: http://dx.doi.org/10.13039/501100000024, Grant Number: 353989).
Acknowledgments
We thank all study participants and their families, without whom this study would not have been possible.
Author contributions: KA and HT conceptualized the research question and designed the study plan. AH, AC, RT, MKB, and JM were involved in participant recruitment and sample collection. VT and SA contributed to retrospective and prospective data analysis, in addition to HT. All authors contributed to drafting and revising the final manuscript. All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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Supplementary Material
The online version of this article offers supplementary material (https://doi.org/10.1515/cclm-2019-0876).
©2020 Walter de Gruyter GmbH, Berlin/Boston
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Articles in the same Issue
- Frontmatter
- Editorial
- New insights on the analytical performances for detecting and quantifying monoclonal proteins
- Reviews
- Vitamin C measurement in critical illness: challenges, methodologies and quality improvements
- Early predictors of perinatal brain damage: the role of neurobiomarkers
- Opinion Paper – Point
- Mixing studies for lupus anticoagulant: mostly yes, sometimes no
- Opinion Paper – Counterpoint
- Mixing studies for lupus anticoagulant: mostly no, sometimes yes
- EFLM Consensus Paper
- Quantifying atherogenic lipoproteins for lipid-lowering strategies: consensus-based recommendations from EAS and EFLM
- Guidelines and Recommendations
- PREDICT: a checklist for preventing preanalytical diagnostic errors in clinical trials
- Genetics and Molecular Diagnostics
- Plasma vs. serum in circulating tumor DNA measurement: characterization by DNA fragment sizing and digital droplet polymerase chain reaction
- General Clinical Chemistry and Laboratory Medicine
- An international multi-center serum protein electrophoresis accuracy and M-protein isotyping study. Part I: factors impacting limit of quantitation of serum protein electrophoresis
- An international multi-center serum protein electrophoresis accuracy and M-protein isotyping study. Part II: limit of detection and follow-up of patients with small M-proteins
- Use of clinical data and acceleration profiles to validate pneumatic transportation systems
- National survey on delta checks in clinical laboratories in China
- Assessment of the glomerular filtration rate (GFR) in kidney transplant recipients using Bayesian estimation of the iohexol clearance
- Performance of Afinion HbA1c measurements in general practice as judged by external quality assurance data
- Renal tubular epithelial cells add value in the diagnosis of upper urinary tract pathology
- Reference Values and Biological Variations
- Influence of ethnicity on biochemical markers of health and disease in the CALIPER cohort of healthy children and adolescents
- Cardiovascular Diseases
- Clinical evaluation of capillary B-type natriuretic peptide testing
- Infectious Diseases
- Thrombo-inflammatory prognostic score improves qSOFA for risk stratification in patients with sepsis: a retrospective cohort study
- Corrigendum
- Corrigendum to: Pediatric reference intervals for 29 Ortho VITROS 5600 immunoassays using the CALIPER cohort of healthy children and adolescents
- Letters to the Editor
- Potential risks in fecal microbiota transplantation
- Macro-aspartate aminotransferase syndrome: a case report
- Factitious severe acidosis in a patient, preanalytical considerations and prevention
- Methods for quick, accurate and cost-effective determination of the type 1 diabetes genetic risk score (T1D-GRS)
- Anti-adalimumab and anti-certolizumab antibodies titers after discontinuation of adalimumab: two case reports
- Serum immunoglobulin-A (IgA) concentrations in a general adult population: association with demographics and prevalence of selective IgA deficiency
- Evaluation of PFA-100 closure times in cord blood samples of healthy term and preterm neonates
- Disagreement between direct and indirect potentiometric Na+ determination in infancy and childhood
- Carryover issues with UF-5000 urine flow cytometry – how did we miss it?
