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Clinical evaluation of capillary B-type natriuretic peptide testing

  • Michael Schreinlechner , Anita Mrakovic , Gerhard Laschober , Christina Mayerl , Andrea Griesmacher , Lian van Lippen , Veronique Semjonow , Jeroen Nieuwenhuis , Alexander van Reenen and Johannes Mair EMAIL logo
Published/Copyright: December 5, 2019
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 58 Issue 4

Abstract

Background

Capillary B-type natriuretic peptide (BNP) testing is attractive in outpatient and emergency settings. The aim of this study was to perform an evaluation of the clinical performances of capillary BNP testing as compared with venous whole blood and plasma point-of-care (POC) BNP as well as plasma N-terminal (NT) proBNP central laboratory testing.

Methods

BNP was measured with a novel single epitope POC assay (Minicare® BV, Eindhoven, The Netherlands) and NT-proBNP with a central laboratory assay (Roche Diagnostics®, Vienna, Austria).

Results

BNP and NT-proBNP were measured in 269 patients of a Department of Cardiology (mean age 67.9 ± 13 years, 26.4% females). Capillary BNP very closely correlated with whole blood venous BNP (r = 0.99, p < 0.001). There was also a close correlation of plasma BNP and NT-proBNP concentrations (r = 0.79, p < 0.001). The diagnostic performances of capillary BNP, whole blood venous BNP, plasma BNP and plasma NT-proBNP for acute heart failure (areas under receiver operating characteristic curves [AUC ROC]: 0.73–0.77) or systolic left ventricular dysfunction in the whole study population (AUC ROC: 0.72–0.76) did not differ significantly. All were significant independent predictors of cardiovascular death during follow-up of the whole study population.

Conclusions

Our study for the first time demonstrated a very close correlation of capillary and venous whole blood or plasma BNP concentrations using the same BNP assay in a large patient cohort. The diagnostic performances of different BNP specimens did not differ significantly, and no significant differences between BNP and NT-proBNP were found either.

Keywords: B-type natriuretic peptide; capillary; N-terminal pro B-type natriuretic peptide; point-of care testing
Corresponding author: Johannes Mair, MD, Department of Internal Medicine III – Cardiology and Angiology, Medical University Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria, Phone: +(512) 504 24118; Fax: +(512) 504 22767

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission. Data analysis, interpretation and writing of the manuscript was performed from co-authors of the Department of Internal Medicine III – Cardiology and Angiology, Medical University Innsbruck, A-6020 Innsbruck Austria.

  2. Research funding: This clinical study was supported by Philips BG Emerging Businesses (Eindhoven, The Netherlands) by financing a study nurse for patient screening and performing BNP point-of-care testing and providing the required reagents, equipment and training.

  3. Employment or leadership: Some co-authors (LvL, VS, JN, AvR) were employees of Philips BG Emerging Businesses at the time when this study was carried out.

  4. Honorarium: None declared.

  5. Competing interests: The funding organisation(s) played no role in the analysis and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/cclm-2019-0672).

Received: 2019-07-03
Accepted: 2019-10-31
Published Online: 2019-12-05
Published in Print: 2020-03-26

©2020 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/cclm-2019-0672
Keywords for this article
B-type natriuretic peptide; capillary; N-terminal pro B-type natriuretic peptide; point-of care testing

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. New insights on the analytical performances for detecting and quantifying monoclonal proteins
  4. Reviews
  5. Vitamin C measurement in critical illness: challenges, methodologies and quality improvements
  6. Early predictors of perinatal brain damage: the role of neurobiomarkers
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  8. Mixing studies for lupus anticoagulant: mostly yes, sometimes no
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  11. EFLM Consensus Paper
  12. Quantifying atherogenic lipoproteins for lipid-lowering strategies: consensus-based recommendations from EAS and EFLM
  13. Guidelines and Recommendations
  14. PREDICT: a checklist for preventing preanalytical diagnostic errors in clinical trials
  15. Genetics and Molecular Diagnostics
  16. Plasma vs. serum in circulating tumor DNA measurement: characterization by DNA fragment sizing and digital droplet polymerase chain reaction
  17. General Clinical Chemistry and Laboratory Medicine
  18. An international multi-center serum protein electrophoresis accuracy and M-protein isotyping study. Part I: factors impacting limit of quantitation of serum protein electrophoresis
  19. An international multi-center serum protein electrophoresis accuracy and M-protein isotyping study. Part II: limit of detection and follow-up of patients with small M-proteins
  20. Use of clinical data and acceleration profiles to validate pneumatic transportation systems
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