Commutability of proficiency testing material containing tobramycin: a study within the framework of the Dutch Calibration 2.000 project
-
Karen Robijns
,
Niels W. Boone
Abstract
Background:
Results from external quality assessment schemes (EQASs) can provide information about accuracy and comparability of different measurement methods, provided that the material used in these schemes behave identical to patient samples among the different methods, a characteristic also known as commutability. The aim of this study was to assess the commutability of different matrices for the material used in an EQAS for tobramycin.
Methods:
Proficiency testing material (PTM) and patient samples containing tobramycin were prepared, collected, pooled, and distributed to participating laboratories for analysis. Low, medium, and high tobramycin concentrations in liquid human, liquid bovine and lyophilized bovine serum were tested in this study. The patient serum results of every laboratory were plotted against each of the other laboratories, and the distances of the PTM results to the patient serum regression line were calculated. For comparison, these distances were divided by the average within-laboratory standard deviation (SDwl) of the results reported in the official EQAS for tobramycin, resulting in a relative residual. The commutability decision limit was set at 3 SDwl.
Results:
With 10 laboratories participating in this study, 45 laboratory couples were formed. For human serum, only one relative residual for high concentrations of tobramycin was found outside the commutability decision limit. For liquid and lyophilized bovine sera, the number of relative residuals outside the decision limit was between 15 and 18 for low, medium, and high tobramycin concentrations.
Conclusions:
The PTM used for tobramycin is preferably prepared with human serum.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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Supplemental Material:
The online version of this article (DOI: 10.1515/cclm-2015-1254) offers supplementary material, available to authorized users.
©2017 Walter de Gruyter GmbH, Berlin/Boston
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Artikel in diesem Heft
- Frontmatter
- Editorial
- Commutable samples with assigned target concentrations may help us harmonise general chemistry results
- Reviews
- Performance of point-of-care HbA1c test devices: implications for use in clinical practice – a systematic review and meta-analysis
- Cardiac troponins and mortality in type 1 and 2 myocardial infarction
- Opinion Paper
- Criteria for assigning laboratory measurands to models for analytical performance specifications defined in the 1st EFLM Strategic Conference
- Genetics and Molecular Diagnostics
- External quality assessment for human papillomavirus 16/18 DNA detection and genotyping in Shanghai, China
- General Clinical Chemistry and Laboratory Medicine
- Analytical performance of 17 general chemistry analytes across countries and across manufacturers in the INPUtS project of EQA organizers in Italy, the Netherlands, Portugal, United Kingdom and Spain
- Commutability of proficiency testing material containing tobramycin: a study within the framework of the Dutch Calibration 2.000 project
- Optimization and validation of moving average quality control procedures using bias detection curves and moving average validation charts
- Extending laboratory automation to the wards: effect of an innovative pneumatic tube system on diagnostic samples and transport time
- Smart management of sample dilution using an artificial neural network to achieve streamlined processes and saving resources: the automated nephelometric testing of serum free light chain as case study
- An integrated proteomic and peptidomic assessment of the normal human urinome
- An alternative inhibition method for determining cross-reactive allergens
- Validation of a new assay for α-synuclein detection in cerebrospinal fluid
- Reference Values and Biological Variations
- Intra-individual variation of plasma trimethylamine-N-oxide (TMAO), betaine and choline over 1 year
- Cancer Diagnostics
- Predictive performance of TPA testing for recurrent disease during follow-up after curative intent surgery for colorectal carcinoma
- Cardiovascular Diseases
- Mid-regional pro-adrenomedullin (MR-proADM) and mid-regional pro-atrial natriuretic peptide (MR-proANP) in severe aortic valve stenosis: association with outcome after transcatheter aortic valve implantation (TAVI)
- Association between apolipoprotein E polymorphisms and premature coronary artery disease: a meta-analysis
- Urinary orosomucoid: a novel, early biomarker of sepsis with promising diagnostic performance
- Letters to the Editor
- CT or MRI
- Reply to: CT or MRI in the diagnosis of right lower quadrant abdominal pain?
- Quantification of daratumumab in the serum protein electrophoresis
- Response to: Interference of daratumumab on the serum protein electrophoresis
- Glycated albumin: correlation to HbA1c and preliminary reference interval evaluation
- Using “big data” to describe the effect of seasonal variation in thyroid-stimulating hormone
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