Startseite Medizin Glucose is stable during prolonged storage in un-centrifuged Greiner tubes with liquid citrate buffer, but not in serum and NaF/KOx tubes
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Glucose is stable during prolonged storage in un-centrifuged Greiner tubes with liquid citrate buffer, but not in serum and NaF/KOx tubes

  • Gordana Juricic EMAIL logo , Ana Bakliza , Andrea Saracevic , Lara Milevoj Kopcinovic , Sanja Dobrijevic , Sandra Drmic und Ana-Maria Simundic
Veröffentlicht/Copyright: 22. Dezember 2015
Clinical Chemistry and Laboratory Medicine (CCLM)
Aus der Zeitschrift Clinical Chemistry and Laboratory Medicine (CCLM) Band 54 Heft 3

Abstract

Background: Delayed sample processing can affect accurate glucose measurement. Our aim was to investigate the stability of glucose in samples collected in serum, sodium fluoride/potassium oxalate (NaF/KOx) and Glucomedics tubes processed according to different controlled pre-centrifugation delays (up to 180 min after venipuncture) in order to simulate prolonged sample transport between venipuncture and centrifugation.

Methods: Samples were collected from healthy volunteers (n=80) into either serum or NaF/KOx and Glucomedics tubes. Glucose concentration was measured in samples centrifuged immediately after venipuncture and compared with tubes processed with a delay of 60, 120 and 180 min prior to centrifugation. Differences between baseline and respective delayed centrifugation glucose value for each tube type were tested using the paired t-test. Mean bias calculated for each tube type and delay protocol was compared to recommended quality specifications for glucose (2.2%).

Results: Glucose concentrations measured in all three delayed tube types were lower in comparison to respective baseline glucose concentrations measured in immediately processed tube (p<0.001). The highest decrease in glucose was observed in serum tubes in all specified time points (p<0.001), while glucose was most stable in Glucomedics tubes (p<0.001). The decrease in glucose observed for serum and NaF/KOx tubes was clinically significant at all specified time points while the bias for Glucomedics tubes did not exceed the criteria even with a centrifugation delay of 180 min.

Conclusions: Glucose stability in un-centrifuged Glucomedics tubes is much superior to serum and NaF/KOx tubes. Glucomedics tubes can be left un-centrifuged for up to 3 h without affecting glucose concentration.

Keywords: diabetes; Glucomedics; glycolysis; glycolysis inhibitors; preanalytical phase
Corresponding author: Gordana Juricic, Department of Laboratory Diagnostics, General Hospital Pula, Aldo Negri 6, Pula, Croatia, Phone: +385 98 908 5506, E-mail:

Acknowledgments

We thank Marija Culjak for her technical skills in performing all venipunctures.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: This study was supported by the Ministry of Science, Education, and Sports, Republic of Croatia (project number: 134-1340227-0200).

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2015-7-31
Accepted: 2015-11-19
Published Online: 2015-12-22
Published in Print: 2016-3-1

©2016 by De Gruyter

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https://doi.org/10.1515/cclm-2015-0746
Schlagwörter für diesen Artikel
diabetes; Glucomedics; glycolysis; glycolysis inhibitors; preanalytical phase

Artikel in diesem Heft

  1. Frontmatter
  2. Editorial
  3. The way of prostate cancer diagnostics
  4. Review
  5. Statistical methods used in the calculation of geriatric reference intervals: a systematic review
  6. Opinion Paper
  7. The side effects of translational omics: overtesting, overdiagnosis, overtreatment
  8. Genetics and Molecular Diagnostics
  9. Rapid detection of non-deletional mutations causing α-thalassemia by multicolor melting curve analysis
  10. General Clinical Chemistry and Laboratory Medicine
  11. Patient pools and the use of “patient means” are valuable tools in quality control illustrated by a bone-specific alkaline phosphatase assay
  12. Long-term stability of glucose: 96-h study using Terumo Glycaemia tubes
  13. Glucose is stable during prolonged storage in un-centrifuged Greiner tubes with liquid citrate buffer, but not in serum and NaF/KOx tubes
  14. Croatian laboratories have a good knowledge of the proper detection and management of hemolyzed, icteric and lipemic samples
  15. Fetal exposure to ethanol: relationship between ethyl glucuronide in maternal hair during pregnancy and ethyl glucuronide in neonatal meconium
  16. Comparing the effect of isotopically labeled or structural analog internal standards on the performance of a LC-MS/MS method to determine ciclosporin A, everolimus, sirolimus and tacrolimus in whole blood
  17. Relationship between matrix metalloproteinase-9 and oxidative stress in drug-free male schizophrenia: a case control study
  18. Comparison of functional fibrinogen (FF/CFF) and FIBTEM in surgical patients – a retrospective study
  19. Reference Values and Biological Variations
  20. Assessment of serum free light chain levels in healthy adults immediately after marathon running
  21. Pharmacokinetics of a novel dosing regimen of oral melatonin in critically ill patients
  22. Cancer Diagnostics
  23. An epidemiology-based model as a tool to monitor the outbreak of inappropriateness in tumor marker requests: a national scale study
  24. Comparative analysis of prostate cancer specific biomarkers PCA3 and ERG in whole urine, urinary sediments and exosomes
  25. Infectious Diseases
  26. Comparative evaluation of the Aptima HIV-1 Quant Dx assay and COBAS TaqMan HIV-1 v2.0 assay using the Roche High Pure System for the quantification of HIV-1 RNA in plasma
  27. Evaluating the use of procalcitonin in an asymptomatic, HIV-infected antiretroviral therapy-naïve, South African cohort
  28. Diabetes
  29. Early prediction of gestational diabetes: a practical model combining clinical and biochemical markers
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  40. Marked elevation of procalcitonin in a patient with a drug related infusion reaction to rituximab
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