Measurement of amino terminal propeptide of type III procollagen (PIIINP) employing the ADVIA Centaur platform. Validation, reference interval and comparison to UniQ RIA
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Cindy Soendersoe Knudsen
,
Lene Heickendorff
Abstract
Background: Recently, measurement of amino terminal propeptide of type III procollagen (PIIINP) was introduced as a part of the hepatic cirrhotic marker enhanced liver fibrosis™ test on the automated ADVIA Centaur® immunoassay platform (Siemens Healthcare Diagnostics Inc., Tarrytown, NY, USA). In this article, we show that the Centaur PIIINP may be used in place of the much more labor-intensive RIA method, and we present an age stratified reference interval.
Methods: We analyzed four control samples 20 times over a period of 5 days. Centaur PIIINP assay measurements were compared with the widely used UniQ PIIINP RIA assay (Orion Diagnostica, Espoo, Finland) using 55 patient samples (range=3.7–43.3 µg/L). Furthermore, we established a reference interval based on samples from 287 blood donors.
Results: In the concentration range 2.5–11.9 µg/L, the total imprecision was below 8%. Comparison with the UniQ PIIINP RIA assay yielded: Centaur PIIINP µg/L=1.9×(UniQ PIIINP RIA)+0.6 µg/L, r2=0.94. The reference interval for the Centaur PIIINP assay showed no gender difference but was stratified by age [4.0–11.0 µg/L (18–40 years) and 3.5–9.5 µg/L (41–70 years)].
Conclusions: We conclude that the Centaur PIIINP assay is suitable for routine use with our newly defined reference interval. The results obtained by Centaur correlates well with those obtained by the previously employed RIA, though the absolute values are higher.
Birgitte Høvsgaard is acknowledged for expert technical assistance and advice.
Conflict of interest statement
Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article. The company donating the kits had no influence in the design of the study and did not participate in preparation of the manuscript.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
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©2014 by Walter de Gruyter Berlin Boston
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Artikel in diesem Heft
- Frontmatter
- Editorial
- The ten commandments of laboratory testing for emergency physicians
- Review
- Critical role of RAGE in lung physiology and tumorigenesis: a potential target of therapeutic intervention?
- Opinion Papers
- Do we really need high-sensitive troponin immunoassays in the emergency department? Definitely, yes!
- Do we really need high-sensitivity troponin immunoassays in the emergency department? Maybe not
- General Clinical Chemistry and Laboratory Medicine
- Cortisol and cortisone ratio in urine: LC-MS/MS method validation and preliminary clinical application
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- Influence of urine volume on the assessment of intestinal permeability in affected children by multiple sugar probes
- Measurement of amino terminal propeptide of type III procollagen (PIIINP) employing the ADVIA Centaur platform. Validation, reference interval and comparison to UniQ RIA
- Cancer Diagnostics
- Enrichment and enumeration of circulating tumor cells by efficient depletion of leukocyte fractions
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- Cardiovascular Diseases
- The value of clinical and laboratory diagnostics for chest pain patients at the emergency department
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- Association of the GCKR rs780094 polymorphism with metabolic traits including carotid intima-media thickness in Japanese community-dwelling men, but not in women
- Infectious Diseases
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- Letters to the Editor
- A short story on how the H-index may change the fate of scientists and scientific publishing
- A new and effective way for preventing pre-analytical laboratory errors
- Digital immunofluorescence enables automated detection of antinuclear antibody endpoint titers avoiding serial dilution
- Multicenter method evaluation of the ARK™ Methotrexate Immunoassay
- Haemoglobinopathies in nonendemic areas in recent years
- Clinical implications of a recent adjustment to the high-sensitivity cardiac troponin T assay: some results
- Effect of recalibration of the hs-TnT assay on diagnostic performance
