Startseite Formation of nitri- and nitrosylhemoglobin in systems modeling the Maillard reaction
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Formation of nitri- and nitrosylhemoglobin in systems modeling the Maillard reaction

  • Olga V. Kosmachevskaya , Konstantin B. Shumaev , Elvira I. Nasybullina und Alexey F. Topunov EMAIL logo
Veröffentlicht/Copyright: 27. August 2013
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Clinical Chemistry and Laboratory Medicine
Aus der Zeitschrift Clinical Chemistry and Laboratory Medicine Band 52 Heft 1

Abstract

Background: Nitric oxide (NO) and its metabolites can nitrosylate hemoglobin (Hb) through the heme iron. Nitrihemoglobin (nitriHb) can be formed as result of porphyrin vinyl group modification with nitrite. However, in those with diabetes the non-enzymatic glycation of Hb amino acids residues (the Maillard reaction) can take place. The objectives of this study were to investigate effects of the Maillard reaction on the interaction of methemoglobin (metHb) with S-nitrosoglutathione (GSNO) and nitrite.

Methods: Nitrosylhemoglobin production was registered using increasing optical density at 572 nm and compared with 592 nm, and with EPR spectroscopy. Formation of nitriHb was determined using an absorbance band of reduced hemochromogen (582 nm) in the alkaline pyridine solution. Accumulation of fluorescent advanced glycation end-products of Hb was measured through increasing of fluorescence at 385–395 nm (excitation λ=320 nm).

Results: We determined that NO metabolites such as GSNO and nitrite at physiological pH values and aerobic conditions caused modification of metHb porphyrin vinyl groups with nitriHb formation. It was ascertained that this formation was inhibited by superoxide dismutase. In microaerobic conditions metHb was nitrosylated under the action of GSNO or GSNO with methylglyoxal. Nitrite nitrosylated metHb only in the presence of methylglyoxal. It was shown that GSNO inhibited accumulation of fluorescent products which formed during Hb glycation with methylglyoxal.

Conclusions: The assumption was made that intermediates of the Hb glycation reaction play an important role both in vinyl group nitration and in heme iron nitrosylation. Oxygen content in reaction medium is an important factor influencing these processes. These effects can play an important role in pathogenesis of the diseases connected with carbonyl, oxidative and nitrosative stresses.

Keywords: carbonyl stress; hemoglobin; Maillard reaction; methylglyoxal; nitrihemoglobin; nitrosylhemoglobin; S-nitrosoglutathione
Corresponding author: Dr. Alexey F. Topunov, A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, Moscow 119071, Russian Federation, Phone: +7 916 1576367, Fax: +7 495 9542732, E-mail:

The work was supported by the Russian Foundation for Basic Research (grants 12-04-01809 and 13-04-00967).

Conflict of interest statement

Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article. Research support played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

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Received: 2012-11-20
Accepted: 2013-7-26
Published Online: 2013-08-27
Published in Print: 2014-01-01

©2014 by Walter de Gruyter Berlin Boston

Artikel in diesem Heft

  1. Masthead
  2. Masthead
  3. Editorial
  4. Frontiers in research on the Maillard reaction in aging and chronic disease
  5. Reviews
  6. Role of the Maillard reaction in aging and age-related diseases. Studies at the cellular-molecular level
  7. Advanced glycation end-products and skin autofluorescence in end-stage renal disease: a review
  8. Glucosepane: a poorly understood advanced glycation end product of growing importance for diabetes and its complications
  9. Mini Reviews
  10. Post-translational modification derived products (PTMDPs): toxins in chronic diseases?
  11. Site-specific AGE modifications in the extracellular matrix: a role for glyoxal in protein damage in diabetes
  12. Augmentation of blood lipid glycation and lipid oxidation in diabetic patients
  13. Maillard reaction products: some considerations on their health effects
  14. The Maillard reaction and food allergies: is there a link?
  15. Perspectives
  16. Chelation therapy for the management of diabetic complications: a hypothesis and a proposal for clinical laboratory assessment of metal ion homeostasis in plasma
  17. Genetics and Molecular Diagnostics
  18. Genetic variability in enzymes of metabolic pathways conferring protection against non-enzymatic glycation versus diabetes-related morbidity and mortality
  19. General Clinical Chemistry and Laboratory Medicine
  20. Quantification of glyoxal, methylglyoxal and 3-deoxyglucosone in blood and plasma by ultra performance liquid chromatography tandem mass spectrometry: evaluation of blood specimen
  21. A new HPLC-based assay for the measurement of fructosamine-3-kinase (FN3K) and FN3K-related protein activity in human erythrocytes
  22. Increased circulating advanced glycation endproducts (AGEs) in acute trauma patients
  23. Circulating soluble RAGE increase after a cerebrovascular event
  24. Pentosidine determination in CSF: a potential biomarker of Alzheimer’s disease?
  25. Cardiovascular Diseases
  26. Skin autofluorescence as proxy of tissue AGE accumulation is dissociated from SCORE cardiovascular risk score, and remains so after 3 years
  27. Plasma advanced glycation end products (AGEs) and NF-κB activity are independent determinants of diastolic and pulse pressure
  28. Total plasma Nε-(carboxymethyl)lysine and sRAGE levels are inversely associated with a number of metabolic syndrome risk factors in non-diabetic young-to-middle-aged medication-free subjects
  29. Translational Research Papers
  30. Advanced glycation end-products induce endoplasmic reticulum stress in human aortic endothelial cells
  31. Formation of nitri- and nitrosylhemoglobin in systems modeling the Maillard reaction
  32. Skin aging by glycation: lessons from the reconstructed skin model
  33. How to help the skin cope with glycoxidation
https://doi.org/10.1515/cclm-2012-0792
Schlagwörter für diesen Artikel
carbonyl stress; hemoglobin; Maillard reaction; methylglyoxal; nitrihemoglobin; nitrosylhemoglobin; S-nitrosoglutathione

Artikel in diesem Heft

  1. Masthead
  2. Masthead
  3. Editorial
  4. Frontiers in research on the Maillard reaction in aging and chronic disease
  5. Reviews
  6. Role of the Maillard reaction in aging and age-related diseases. Studies at the cellular-molecular level
  7. Advanced glycation end-products and skin autofluorescence in end-stage renal disease: a review
  8. Glucosepane: a poorly understood advanced glycation end product of growing importance for diabetes and its complications
  9. Mini Reviews
  10. Post-translational modification derived products (PTMDPs): toxins in chronic diseases?
  11. Site-specific AGE modifications in the extracellular matrix: a role for glyoxal in protein damage in diabetes
  12. Augmentation of blood lipid glycation and lipid oxidation in diabetic patients
  13. Maillard reaction products: some considerations on their health effects
  14. The Maillard reaction and food allergies: is there a link?
  15. Perspectives
  16. Chelation therapy for the management of diabetic complications: a hypothesis and a proposal for clinical laboratory assessment of metal ion homeostasis in plasma
  17. Genetics and Molecular Diagnostics
  18. Genetic variability in enzymes of metabolic pathways conferring protection against non-enzymatic glycation versus diabetes-related morbidity and mortality
  19. General Clinical Chemistry and Laboratory Medicine
  20. Quantification of glyoxal, methylglyoxal and 3-deoxyglucosone in blood and plasma by ultra performance liquid chromatography tandem mass spectrometry: evaluation of blood specimen
  21. A new HPLC-based assay for the measurement of fructosamine-3-kinase (FN3K) and FN3K-related protein activity in human erythrocytes
  22. Increased circulating advanced glycation endproducts (AGEs) in acute trauma patients
  23. Circulating soluble RAGE increase after a cerebrovascular event
  24. Pentosidine determination in CSF: a potential biomarker of Alzheimer’s disease?
  25. Cardiovascular Diseases
  26. Skin autofluorescence as proxy of tissue AGE accumulation is dissociated from SCORE cardiovascular risk score, and remains so after 3 years
  27. Plasma advanced glycation end products (AGEs) and NF-κB activity are independent determinants of diastolic and pulse pressure
  28. Total plasma Nε-(carboxymethyl)lysine and sRAGE levels are inversely associated with a number of metabolic syndrome risk factors in non-diabetic young-to-middle-aged medication-free subjects
  29. Translational Research Papers
  30. Advanced glycation end-products induce endoplasmic reticulum stress in human aortic endothelial cells
  31. Formation of nitri- and nitrosylhemoglobin in systems modeling the Maillard reaction
  32. Skin aging by glycation: lessons from the reconstructed skin model
  33. How to help the skin cope with glycoxidation
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