Skin aging by glycation: lessons from the reconstructed skin model
-
Hervé Pageon
,
Hélène Zucchi
Abstract
Background: Aging is the result of several mechanisms which operate simultaneously. Among them, glycation is of particular interest because it is a reaction which affects slowly renewing tissues and macromolecules with elevated half-life, like the dermis, a skin compartment highly affected by aging. Glycation produces crosslinks between macromolecules thereby providing an explanation for the increased age-related stiffness of the skin. Glycation products, also called AGEs (advanced glycation end products), accumulate primarily in extracellular matrix molecules like collagen or elastin.
Methods: In order to reproduce this phenomenon in vitro we have created a model of reconstructed skin modified by glycation of the collagen used to fabricate the dermal compartment.
Results: This system allowed us to uncover biological modifications of dermal markers, and more surprisingly epidermal markers, as well as an increase of metalloproteinases responsible for degradation of the dermal matrix. Consequently, the imbalance between synthesis and degradation that results from glycation, may contribute to skin aging, as shown in this model. Moreover these modifications were shown to be prevented by the addition of aminoguanidine, a well-known inhibitor of glycation.
Conclusions: Using this experimental approach our results taken together stress the importance and possibly central role of glycation in skin aging and the usefulness of the reconstructed skin as a model of physiological aging.
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©2014 by Walter de Gruyter Berlin Boston
Artikel in diesem Heft
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Artikel in diesem Heft
- Masthead
- Masthead
- Editorial
- Frontiers in research on the Maillard reaction in aging and chronic disease
- Reviews
- Role of the Maillard reaction in aging and age-related diseases. Studies at the cellular-molecular level
- Advanced glycation end-products and skin autofluorescence in end-stage renal disease: a review
- Glucosepane: a poorly understood advanced glycation end product of growing importance for diabetes and its complications
- Mini Reviews
- Post-translational modification derived products (PTMDPs): toxins in chronic diseases?
- Site-specific AGE modifications in the extracellular matrix: a role for glyoxal in protein damage in diabetes
- Augmentation of blood lipid glycation and lipid oxidation in diabetic patients
- Maillard reaction products: some considerations on their health effects
- The Maillard reaction and food allergies: is there a link?
- Perspectives
- Chelation therapy for the management of diabetic complications: a hypothesis and a proposal for clinical laboratory assessment of metal ion homeostasis in plasma
- Genetics and Molecular Diagnostics
- Genetic variability in enzymes of metabolic pathways conferring protection against non-enzymatic glycation versus diabetes-related morbidity and mortality
- General Clinical Chemistry and Laboratory Medicine
- Quantification of glyoxal, methylglyoxal and 3-deoxyglucosone in blood and plasma by ultra performance liquid chromatography tandem mass spectrometry: evaluation of blood specimen
- A new HPLC-based assay for the measurement of fructosamine-3-kinase (FN3K) and FN3K-related protein activity in human erythrocytes
- Increased circulating advanced glycation endproducts (AGEs) in acute trauma patients
- Circulating soluble RAGE increase after a cerebrovascular event
- Pentosidine determination in CSF: a potential biomarker of Alzheimer’s disease?
- Cardiovascular Diseases
- Skin autofluorescence as proxy of tissue AGE accumulation is dissociated from SCORE cardiovascular risk score, and remains so after 3 years
- Plasma advanced glycation end products (AGEs) and NF-κB activity are independent determinants of diastolic and pulse pressure
- Total plasma Nε-(carboxymethyl)lysine and sRAGE levels are inversely associated with a number of metabolic syndrome risk factors in non-diabetic young-to-middle-aged medication-free subjects
- Translational Research Papers
- Advanced glycation end-products induce endoplasmic reticulum stress in human aortic endothelial cells
- Formation of nitri- and nitrosylhemoglobin in systems modeling the Maillard reaction
- Skin aging by glycation: lessons from the reconstructed skin model
- How to help the skin cope with glycoxidation
