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Reference change values for lens culinaris agglutinin-reactive α-fetoprotein and des-γ-carboxy prothrombin in patients with chronic hepatitis C

  • Futoshi Kanke EMAIL logo , Takashi Kumada , Hidenori Toyoda and Shinji Satomura
Published/Copyright: March 6, 2012

Abstract

Background:Lens culinaris agglutinin-reactive α-fetoprotein (AFP-L3) and des-γ-carboxy prothrombin (DCP) have been routinely used as serological tumor markers of hepatocellular carcinoma (HCC) for surveillance. The aims of this study were: (i) to determine the biological variation of AFP-L3 and DCP in patients with chronic hepatitis C; and (ii) to calculate the reference change values (RCVs) of AFP-L3 and DCP.

Methods: Ten patients with cirrhosis due to hepatitis C virus (HCV) infection and without HCC were enrolled in the study. Serum samples were collected at 14-day intervals, and 10 samples in total were obtained for each patient. AFP-L3 and DCP levels were measured by microchip capillary electrophoresis and liquid-phase binding assay. Intra-individual (CVI) and inter-individual (CVG) biological variations and RCVs were estimated from the data generated.

Results: The CVI was 29.0% for AFP-L3 and 24.6% for DCP, and CVG was 63.5% for AFP-L3 and 40.4% for DCP. The RCVs for AFP-L3 and DCP were 68.3% and 58.5%, respectively.

Conclusions: Increases in values for AFP-L3 and DCP within 68.3% and 58.5% may be biological variations. Clinician should take these variations into consideration for the management of patients with HCV infection under surveillance of HCC.


Corresponding author: Futoshi Kanke, Diagnostics Division, Wako Pure Chemical Industries Ltd., 1-2, Doshomachi 3, Chuo-ku, Osaka 540-8605, Japan Fax: +81 6 62031917

Received: 2011-5-9
Accepted: 2012-1-27
Published Online: 2012-03-06
Published in Print: 2012-05-01

©2012 by Walter de Gruyter Berlin Boston

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