A taxonomic re-examination of Saccharina longipedalis (Laminariales, Phaeophyceae), an endemic kelp species around Lake Akkeshi in eastern Hokkaido, Japan
-
Norishige Yotsukura
Norishige Yotsukura is an Associate Professor in Field Science Center for Northern Biosphere, Hokkaido University. He also serves as the Director of Oshoro Marine Station, Hokkaido University. His research interests are on biodiversity, conservation of natural resources and breeding of kelp.
,
Nobu Nagai
Nobu Nagai earned both his Bachelor’s degree and Master’s degree from Hokkaido University. His Master’s research was focused on biodiversity of kelp in the Russian Far East. He currently works in a leading baking company in Japan.
Tadashi Kawai is an Assistant Chief of the aquaculture and resources enhancement section on seaweeds, shellfish and sea urchin in Hokkaido Research Organization. He also has interests in biology, taxonomy, and conservation of freshwater crayfish.
Abstract
The taxonomy of Saccharina longipedalis, a kelp species endemic to the brackish Lake Akkeshi in eastern Hokkaido, was reviewed. The external morphology of sporophytes collected from seven sites around the distribution boundary of S. longipedalis and Saccharina japonica var. diabolica were compared; it was confirmed that the diagnostic morphology of S. longipedalis, such as a long stipe and thin blade, was not solely characteristic of individuals that grew in and around the center of the lake, which is thought to be the unique distribution area of S. longipedalis. Sequence comparisons of a 5S rDNA spacer and four microsatellite regions and AFLP analysis revealed that genetic similarity was high between S. longipedalis and S. japonica var. diabolica. Also based on observations that the early development in hybrids of sporelings from the seven sites was normal, we conclude that S. longipedalis should be treated as S. japonica var. diabolica.
About the authors
Norishige Yotsukura is an Associate Professor in Field Science Center for Northern Biosphere, Hokkaido University. He also serves as the Director of Oshoro Marine Station, Hokkaido University. His research interests are on biodiversity, conservation of natural resources and breeding of kelp.
Nobu Nagai earned both his Bachelor’s degree and Master’s degree from Hokkaido University. His Master’s research was focused on biodiversity of kelp in the Russian Far East. He currently works in a leading baking company in Japan.
Tadashi Kawai is an Assistant Chief of the aquaculture and resources enhancement section on seaweeds, shellfish and sea urchin in Hokkaido Research Organization. He also has interests in biology, taxonomy, and conservation of freshwater crayfish.
Acknowledgments
The authors thank Mr. H. Gouda (Hokkaido Research Organization) and Mr. M. Kimura (Hokkaido Prefecture) for useful comments. The authors also thank Mr. S. Hamano and Mr. H. Katsuragawa (Hokkaido University) for collecting materials.
References
Duggins, D.O., J.E. Eckman, C.E. Siddon and T. Klinger. 2003. Population, morphometric and biomechanical studies of three understory kelps along a hydrodynamic gradient. Mar. Ecol. Prog.Ser. 265: 57–76.10.3354/meps265057Search in Google Scholar
Fowler-Walker, M.J., S.D. Connell and B.M. Gillanders. 2005. To what extent do geographic and associated environmental variables correlate with kelp morphology across temperate Australia? Mar. Freshw. Res.56: 877–887.10.1071/MF05042Search in Google Scholar
Funano, T. 1991. Morpholoical and ecological comparisons of Laminaria religiosa, a survival variation of L. japonica and that of L. ochotensis from Oshoro, Sutts and Atsuta. Suisanzoshoku39: 91–95 (in Japanese).Search in Google Scholar
Kawashima, S. 1994. Taxonomic and distributional remarks on the laminariaceous algae (Laminariales, Phyaeophyta) of Japan-47. Aquabiology 91: 103–106 (in Japanese).Search in Google Scholar
Kawashima, S. 1995a. Taxonomic and distributional remarks on the laminariaceous algae (Laminariales, Phyaeophyta) of Japan-51. Aquabiology 95: 464–467 (in Japanese).Search in Google Scholar
Kawashima, S. 1995b. Taxonomic and distributional remarks on the laminariaceous algae (Laminariales, Phyaeophyta) of Japan-52. Aquabiology 95: 7–10 (in Japanese).Search in Google Scholar
Kawashima, S. 1995c. Taxonomic and distributional remarks on the laminariaceous algae (Laminariales, Phyaeophyta) of Japan-53. Aquabiology 97: 111–115 (in Japanese).Search in Google Scholar
Lane, C.E., C. Mayes, L.D. Druehl and G.W. Saunders. 2006. A multi-gene molecular investigation of the kelp (Laminariales, Phaeophyceae) supports substantial taxonomic re-organization. J. Phycol. 42: 493–512.10.1111/j.1529-8817.2006.00253.xSearch in Google Scholar
Maeda, T. and N. Yotsukura. 2013. Development of microsatellite markers for Saccharina japonica by dual-suppression PCR. Algal Res.6: 67–71.Search in Google Scholar
Maeda, T., T. Kawai, M. Nakaoka and N. Yotsukura. 2013. Effective DNA extraction method for fragment analysis using capillary sequencer of the kelp, Saccharina. J. Appl. Phycol. 25: 337–347.10.1007/s10811-012-9868-3Search in Google Scholar
Miyabe, K. 1902. Laminariaceae. Hokkaido Suisan Chosahokoku Vol. 3. Hokkaido Shokuminbu, Sapporo. pp. 216 (in Japanese).Search in Google Scholar
Nabata, S., E. Abe and M. Kakiuchi. 1993. Ecological study and transplanting experiment of the biennial plants of Laminaria on the coast of Isoya, Suttu, southernwestern Hokkaido, Japan. Sci. Rep. Hokkaido Fish. Exp. Stn. 43: 25–35.Search in Google Scholar
Notoya, M. and S. Kirihara. 1989. Morphology of wild and transplantation plants of Laminaria japonica at the coast of Aomori prefecture. Kaiyoukagaku21: 355–361 (in Japanese).Search in Google Scholar
Okamura, K. 1891. On seaweeds of Hokkaido. Dainippon Suisankaihokoku114: 584–590 (in Japanese).Search in Google Scholar
Okamura, K. 1896. On Laminaria of Japan. Bot. Mag. Tokyo10: 87–101.10.15281/jplantres1887.10.117_87Search in Google Scholar
Okamura, K. 1916. Nippon Sorui Meii. Seibido, Tokyo. pp. 276 (in Japanese).Search in Google Scholar
Sanbonsuga, Y. 1978. On the morphological characteristics of Laminaria japonica var. japonica studied by transplanting experiment. II. On the varieties of Laminaria japonica var. japonica Aresch. Bull. Hokkaido Reg. Fish. Res. Lab.43: 79–88.Search in Google Scholar
Sanbonsuga, Y. and S. Torii. 1973. On the morphological characteristics of Laminaria japonica var. japonica studied by transplanting experiment. I. On local forms of Laminaria japonica var. japonica. Bull. Hokkaido Reg. Fish. Res. Lab.39: 61–82.Search in Google Scholar
Sanbonsuga, Y. and S. Torii. 1974. On the local variation of morphological characteristics in Laminaria japonica Areschoug var. japonica. Bull. Hokkaido Reg. Fish. Res. Lab. 40: 48–59.Search in Google Scholar
Ueda, S. 1933. Marine botany. Kouseikaku, Tokyo. pp. 319 (in Japanese).Search in Google Scholar
Yendo, K. 1911. Studies of marine vegetation. Hakubunkan, Tokyo. pp. 748 (in Japanese).Search in Google Scholar
Yoon, H.S., J.Y. Lee, S.M. Boo and D. Bhattacharya. 2001. Phylogeny of Alariaceae, Laminariaceae, and Lessoniaceae (Phaeophyceae) based on plastid-encoded RuBisCo spacer and nuclear-encoded ITS sequence comparisons. Mol. Phylogenet. Evol. 21: 231–243.10.1006/mpev.2001.1009Search in Google Scholar PubMed
Yoshida, T., M. Suzuki and K. Yoshinaga. 2015. Check list of marine algae of Japan (Revised in 2015). Jpn. J. Phycol. 63: 129–189 (in Japanese).Search in Google Scholar
Yotsukura, N. 2005. Molecular phylogeny of advanced kelps (Laminariales, Phaeophyceae) growing in Japan. Nat. Hist. Res.8: 69–81.Search in Google Scholar
Yotsukura, N. and L.D. Druehl. 2009. A new name for Laminaria sachalinensis (Laminariales, Phaeophyceae). J. Jpn. Bot. 84: 113–117.Search in Google Scholar
Yotsukura, N., T. Denboh, T. Motomura, T. Horiguchi, A.W. Coleman and T. Ichimura. 1999. Little divergence in ribosomal DNA internal transcribed spacer –1 and –2 sequences among non-digitate species of Laminaria (Phaeophyceae) from Hokkaido, Japan. Phycol. Res.47: 71–80.10.1111/j.1440-1835.1999.tb00286.xSearch in Google Scholar
Yotsukura, N., T. Kawai, S. Kawashima, H. Ebata and T. Ichimura. 2006. Nucleotide sequences diversity of the 5S rDNA spacer in the simple blade genera Laminaria, Cymathaere and Kjellmaniella (Laminariales, Phaeophyceae) from northern Japan. Phycol. Res.54: 269–279.10.1111/j.1440-1835.2006.00434.xSearch in Google Scholar
Yotsukura, N., S. Kawashima, T. Kawai, T. Abe and L.D. Druehl. 2008. A systematic re-examination of four Laminaria species: L. japonica, L. religiosa, L. ochotensis and L. diabolica. J. Jpn. Bot.83: 165–176.Search in Google Scholar
Yotsukura, N., T. Shimizu, T. Katayama, L.D. Druehl. 2010. Mitochondrial DNA sequence variation of four Saccharina species (Laminariales, Phaeophyceae) growing in Japan. J. Appl. Phycol. 22: 243–251.10.1007/s10811-009-9452-7Search in Google Scholar
Yotsukura, N., T. Maeda, T. Abe, M. Nakaoka and T. Kawai. 2016. Detection of genetic difference among varieties in Saccharina japonica in northern Japan by DNA fragment analyses. J. Appl. Phycol.10.1007/s10811-016-0807-6Search in Google Scholar
©2016 by De Gruyter
Articles in the same Issue
- Frontmatter
- In this issue
- Research articles
- Influences of hydrozoan colonization on proteomic profiles of the brown alga Saccharina japonica
- Light and temperature requirements for survival, growth and reproduction of the crustose coralline Lithophyllum stictaeforme from the Mediterranean Sea
- New records and observations of macroalgae and associated pathogens from the Falkland Islands, Patagonia and Tierra del Fuego
- Evidence for the proposed merger of Pseudobryopsis papillata with Pseudobryopsis myura (Bryopsidaceae, Chlorophyta)
- Description of two new species of Ochtodes (Rhodophyta: Gigartinales) from Caribbean Mexico on the basis of morphological and molecular evidence
- Molecular and morphological characterisation of Ulva chaugulii, U. paschima and U. ohnoi (Ulvophyceae) from the Persian Gulf, Iran
- Scrippsiella trochoidea cysts in recent sediments from Amur Bay, Sea of Japan: distribution and phylogeny
- Biomass and productivity of seagrasses in Africa
- Photosynthesis and calcification in two Halimeda species from Phuket, Thailand
- Short communications
- Flowering and seed production in the subtropical seagrass, Halodule wrightii (shoal grass)
- Plastid terminal oxidase acts as an alternative electron sink in a marine cyanobacterium Arthrospira sp.
- A taxonomic re-examination of Saccharina longipedalis (Laminariales, Phaeophyceae), an endemic kelp species around Lake Akkeshi in eastern Hokkaido, Japan
Articles in the same Issue
- Frontmatter
- In this issue
- Research articles
- Influences of hydrozoan colonization on proteomic profiles of the brown alga Saccharina japonica
- Light and temperature requirements for survival, growth and reproduction of the crustose coralline Lithophyllum stictaeforme from the Mediterranean Sea
- New records and observations of macroalgae and associated pathogens from the Falkland Islands, Patagonia and Tierra del Fuego
- Evidence for the proposed merger of Pseudobryopsis papillata with Pseudobryopsis myura (Bryopsidaceae, Chlorophyta)
- Description of two new species of Ochtodes (Rhodophyta: Gigartinales) from Caribbean Mexico on the basis of morphological and molecular evidence
- Molecular and morphological characterisation of Ulva chaugulii, U. paschima and U. ohnoi (Ulvophyceae) from the Persian Gulf, Iran
- Scrippsiella trochoidea cysts in recent sediments from Amur Bay, Sea of Japan: distribution and phylogeny
- Biomass and productivity of seagrasses in Africa
- Photosynthesis and calcification in two Halimeda species from Phuket, Thailand
- Short communications
- Flowering and seed production in the subtropical seagrass, Halodule wrightii (shoal grass)
- Plastid terminal oxidase acts as an alternative electron sink in a marine cyanobacterium Arthrospira sp.
- A taxonomic re-examination of Saccharina longipedalis (Laminariales, Phaeophyceae), an endemic kelp species around Lake Akkeshi in eastern Hokkaido, Japan
