The influence of biocomposites containing genetically modified flax fibers on gene expression in rat skeletal muscle
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Tomasz Gredes
Abstract
In many studies, natural flax fibers have been proven to be resistant and surgically suitable. Genetically modified flax fibers, derived from transgenic flax expressing three bacterial genes for the synthesis of poly-3-hydroxybutyric acid (PHB), have better mechanical properties than unmodified flax fibers. The aim of this study was to examine the biocompatibility of composites containing flax fibers from transgenic polyhydroxybutyrate producing (M50) and control (wt-NIKE) plants in a polylactide (PLA) matrix in rat Musculus latissimus dorsi. For this purpose, effects of biocomposites on the expression of growth factors and osteogenic differentiation, in particular the mRNA expression of vascular endothelial growth factor, insulin like growth factor 1, insulin like growth factor 2, collagen-1, collagen-2 and myostatin, were analyzed using quantitative RT-PCR. The biocomposites did not show any inflammation response after subcutaneous insertion. The results following subcutaneous insertion of PLA alone and PLA-M50 showed no significant changes on the gene expression of all tested genes, whereas PLA-wt-NIKE reduced the mRNA amount of myostatin, VEGFA and IGF2, respectively. It can be asserted that modified flax membranes with PHB and other organic substances have a good biocompatibility to the muscle and they do not disrupt the muscle function. Furthermore, composites from transgenic flax plants producing PHB did not differ from composites of non-transgenic flax plants.
©2010 by Walter de Gruyter Berlin New York
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Artikel in diesem Heft
- Guest editorial
- Aspects of oral and dental reconstitution with dental implants and membranes
- Review
- Clinical, biomechanical and biological aspects of immediately loaded dental implants: a critical review of the literature
- Research articles
- Crestal bone resorption around platform-switched dental implants with fine threaded neck after immediate and delayed loading
- The influence of biocomposites containing genetically modified flax fibers on gene expression in rat skeletal muscle
- Use of primary culture of human fibroblasts in gingiva augmentation procedure
- Retention force of secondary crowns to copings after temporary cementation: the effect of crown material and luting agent
- Biomechanical finite element analysis of small diameter and short dental implant
- Original articles
- Systematic user-based assessment of “Navigated Control Spine” / Systematische, nutzerzentrierte Evaluation von „Navigated Control Spine”
- Influence of formalin fixation on the biomechanical properties of human diaphyseal bone
- Asynchronous steady-state visual evoked potential based BCI control of a 2-DoF artificial upper limb
