Use of primary culture of human fibroblasts in gingiva augmentation procedure
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Marzena Dominiak
Abstract
Background: The aim of this study was to assess gingival aesthetic after usage of an own method of primary culture of human fibroblasts derived from the connective tissue of oral cavity keratinized gingiva on collagenous carrier in gingival augmentation procedures.
Materials and methods: Procedures were performed on 10 patients (7 females, 3 males) aged 18–35 years. In total, 34 teeth in the anterior part of the maxilla and the mandible underwent treatment. The protocol consisted of (1) preparing the patient for tissue biopsy, (2) biopsy of keratinized tissue, (3) laboratory tissue culture, (4) application of expanded cells into the recipient site, and (5) post-procedure management. Aesthetic index, pocket depth (PD), and plaque (PI1) and after surgery complications were examined.
Results: There was post-procedure aesthetic improvement in all 34 cases compared with the pre-procedure condition (grade 1), and furthermore a significant decrease in PD and PI1 were revealed.
Conclusion: Use of own method of primary culture of human fibroblasts on a collagenous carrier for gingival augmentation is an aesthetic method of mucogingival complex repair.
©2010 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Guest editorial
- Aspects of oral and dental reconstitution with dental implants and membranes
- Review
- Clinical, biomechanical and biological aspects of immediately loaded dental implants: a critical review of the literature
- Research articles
- Crestal bone resorption around platform-switched dental implants with fine threaded neck after immediate and delayed loading
- The influence of biocomposites containing genetically modified flax fibers on gene expression in rat skeletal muscle
- Use of primary culture of human fibroblasts in gingiva augmentation procedure
- Retention force of secondary crowns to copings after temporary cementation: the effect of crown material and luting agent
- Biomechanical finite element analysis of small diameter and short dental implant
- Original articles
- Systematic user-based assessment of “Navigated Control Spine” / Systematische, nutzerzentrierte Evaluation von „Navigated Control Spine”
- Influence of formalin fixation on the biomechanical properties of human diaphyseal bone
- Asynchronous steady-state visual evoked potential based BCI control of a 2-DoF artificial upper limb
