Abstract
Label-free multiphoton imaging constitutes a promising technique for clinical diagnosis and therapeutic monitoring. Corpora amylacea (CoA) are starch-like structures often found in the diseased brain, whose origin and role in nervous pathologies are still a matter of debate. Recently, CoA in the diseased human hippocampus were found to be second harmonic generation (SHG) active. Here, we show that CoA formed in other parts of the diseased brain and in brain neoplasms display a similar SHG activity. The SHG pattern of CoA depended on laser polarization, indicating that a radial structure is responsible for their nonlinear activity. Vibrational spectroscopy was used to study the biochemistry underlying the SHG activity. Infrared (IR) and Raman spectroscopy showed that CoA contain polyglucosans that are biochemically similar to glycogen, but with an unusual structure that is similar to amylopectin, which justifies the nonlinear activity of CoA. Our findings explain the SHG activity of CoA and demonstrate that CoA in the pathological brain are amenable to label-free multiphoton imaging. Further research will clarify whether intraoperative assessment of CoA can be diagnostically exploited.
Author Statement
Research funding: The research was partly funded by the Bundesministerium für Bildung und Forschung (German Federal Ministry of Education and Research) project EndoCARS (Funder ID: 10.13039/501100002347, AZ: 13N13807).
Conflict of interest: Authors state no conflict of interest.
Informed consent: Human tissue was obtained from surgery for the treatment of pharmacoresistant epilepsy or brain tumor surgery. All patients gave their written consent.
Ethical approval: The research related to human use complied with all the relevant national regulations and institutional policies and was performed in accordance with the tenets of the Helsinki Declaration and has been approved by the Ethics Committee at Dresden University Hospital (EK 323122008).
References
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Guest Editorial
- Optical imaging methods in medicine: how can we escape the plausibility trap?
- Special Issue Articles
- Diffuse near-infrared imaging of tissue with picosecond time resolution
- A compact hyperspectral camera for measurement of perfusion parameters in medicine
- LED for hyperspectral imaging – a new selection method
- Approaches for calibration and validation of near-infrared optical methods for oxygenation monitoring
- Hyperspectral imaging in perfusion and wound diagnostics – methods and algorithms for the determination of tissue parameters
- Algorithms for mapping kidney tissue oxygenation during normothermic machine perfusion using hyperspectral imaging
- Intraoperative mapping of the sensory cortex by time-resolved thermal imaging
- Intraoperative motion correction in neurosurgery: a comparison of intensity- and feature-based methods
- Optical molecular imaging of corpora amylacea in human brain tissue
- Intraoperative optical imaging of metabolic changes after direct cortical stimulation – a clinical tool for guidance during tumor resection?
- Application of optical and spectroscopic technologies for the characterization of carious lesions in vitro
- Hyperspectral imaging: innovative diagnostics to visualize hemodynamic effects of cold plasma in wound therapy
- Hyperspectral imaging as a possible tool for visualization of changes in hemoglobin oxygenation in patients with deficient hemodynamics – proof of concept
- Cardiovascular assessment by imaging photoplethysmography – a review
Articles in the same Issue
- Frontmatter
- Guest Editorial
- Optical imaging methods in medicine: how can we escape the plausibility trap?
- Special Issue Articles
- Diffuse near-infrared imaging of tissue with picosecond time resolution
- A compact hyperspectral camera for measurement of perfusion parameters in medicine
- LED for hyperspectral imaging – a new selection method
- Approaches for calibration and validation of near-infrared optical methods for oxygenation monitoring
- Hyperspectral imaging in perfusion and wound diagnostics – methods and algorithms for the determination of tissue parameters
- Algorithms for mapping kidney tissue oxygenation during normothermic machine perfusion using hyperspectral imaging
- Intraoperative mapping of the sensory cortex by time-resolved thermal imaging
- Intraoperative motion correction in neurosurgery: a comparison of intensity- and feature-based methods
- Optical molecular imaging of corpora amylacea in human brain tissue
- Intraoperative optical imaging of metabolic changes after direct cortical stimulation – a clinical tool for guidance during tumor resection?
- Application of optical and spectroscopic technologies for the characterization of carious lesions in vitro
- Hyperspectral imaging: innovative diagnostics to visualize hemodynamic effects of cold plasma in wound therapy
- Hyperspectral imaging as a possible tool for visualization of changes in hemoglobin oxygenation in patients with deficient hemodynamics – proof of concept
- Cardiovascular assessment by imaging photoplethysmography – a review