Application of optical and spectroscopic technologies for the characterization of carious lesions in vitro
-
Florian Tetschke
,
Lars Kirsten
Abstract
The detection of the beginning demineralization process of dental hard tissue remains a challenging task in dentistry. As an alternative to bitewing radiographs, optical and spectroscopic technologies showed promising results for caries diagnosis. The aim of the present work is to give an overview of optical and spectroscopic properties of healthy and carious human teeth in vitro by means of Raman spectroscopy (RS), optical coherence tomography (OCT) and hyperspectral imaging (HSI). OCT was able to represent microstructural changes below the enamel surface and revealed increased scattering for white spot lesions and a white scattering trail for deeper lesions. HSI showed similar absorbance characteristics for healthy and demineralized enamel over the entire spectrum and a characteristic absorbance peak at 550 nm for discolored lesions. Already at early carious stages (white spot), we found a distinct loss of hydroxylapatite-related intensity at 959 cm−1 in demineralized regions with RS. Healthy and demineralized tooth surfaces can be distinguished at different signal levels by means of RS, OCT and HSI. The presented modalities provide additional information to the current clinical diagnosis of caries such as microstructural changes, quantification of the demineralization and imaging of caries-related chemical changes.
Acknowledgments
The authors would like to thank Dr. med. dent. Marie-Theres Weber, Dr. med. dent. Jasmin Kirsch and Dr. med. dent. Jörg Rietschel for their advice in clinical questions and the support in collection and preparation of extracted teeth. Furthermore, the authors would like to thank Tobias Rosenauer and Julia Ernst for the fruitful discussions on optical technologies in dentistry.
Research funding: This project is supported by the European Union/European Social Fund (ESF) and the Free State of Saxony within the ESF junior research group “Optical Technologies in Medicine” (Funder Id: 10.13039/501100004895, project number 100270108) and within a doctoral scholarship (project number 100284305).
Conflict of interest: Authors state no conflict of interest.
Informed consent: Informed consent is not applicable.
Ethical approval: The conducted research is not related to either human or animal use.
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Guest Editorial
- Optical imaging methods in medicine: how can we escape the plausibility trap?
- Special Issue Articles
- Diffuse near-infrared imaging of tissue with picosecond time resolution
- A compact hyperspectral camera for measurement of perfusion parameters in medicine
- LED for hyperspectral imaging – a new selection method
- Approaches for calibration and validation of near-infrared optical methods for oxygenation monitoring
- Hyperspectral imaging in perfusion and wound diagnostics – methods and algorithms for the determination of tissue parameters
- Algorithms for mapping kidney tissue oxygenation during normothermic machine perfusion using hyperspectral imaging
- Intraoperative mapping of the sensory cortex by time-resolved thermal imaging
- Intraoperative motion correction in neurosurgery: a comparison of intensity- and feature-based methods
- Optical molecular imaging of corpora amylacea in human brain tissue
- Intraoperative optical imaging of metabolic changes after direct cortical stimulation – a clinical tool for guidance during tumor resection?
- Application of optical and spectroscopic technologies for the characterization of carious lesions in vitro
- Hyperspectral imaging: innovative diagnostics to visualize hemodynamic effects of cold plasma in wound therapy
- Hyperspectral imaging as a possible tool for visualization of changes in hemoglobin oxygenation in patients with deficient hemodynamics – proof of concept
- Cardiovascular assessment by imaging photoplethysmography – a review
Articles in the same Issue
- Frontmatter
- Guest Editorial
- Optical imaging methods in medicine: how can we escape the plausibility trap?
- Special Issue Articles
- Diffuse near-infrared imaging of tissue with picosecond time resolution
- A compact hyperspectral camera for measurement of perfusion parameters in medicine
- LED for hyperspectral imaging – a new selection method
- Approaches for calibration and validation of near-infrared optical methods for oxygenation monitoring
- Hyperspectral imaging in perfusion and wound diagnostics – methods and algorithms for the determination of tissue parameters
- Algorithms for mapping kidney tissue oxygenation during normothermic machine perfusion using hyperspectral imaging
- Intraoperative mapping of the sensory cortex by time-resolved thermal imaging
- Intraoperative motion correction in neurosurgery: a comparison of intensity- and feature-based methods
- Optical molecular imaging of corpora amylacea in human brain tissue
- Intraoperative optical imaging of metabolic changes after direct cortical stimulation – a clinical tool for guidance during tumor resection?
- Application of optical and spectroscopic technologies for the characterization of carious lesions in vitro
- Hyperspectral imaging: innovative diagnostics to visualize hemodynamic effects of cold plasma in wound therapy
- Hyperspectral imaging as a possible tool for visualization of changes in hemoglobin oxygenation in patients with deficient hemodynamics – proof of concept
- Cardiovascular assessment by imaging photoplethysmography – a review
