Genetic and morphological diversity in Geranium dissectum (Sec. Dissecta, Geraniaceae) populations
Abstract
Geranium, section Dissecta, (Geraniaceae) consists of four species in the world. It is believed that the center of diversification of this section is Eurasia. G. dissectum is the only species of this section in northern parts of Iran. A multivariate morphometric study showed that some quantitative characters such as deeply divided leaves, shorter and narrower petals, and shorter filaments are main diagnostic features of G. dissectum. This tannin-rich plant has many uses in folk medicine. There is no information on its population genetic structure, genetic diversity, and morphological variability in Iran. Due to the medicinal and weed importance of this species, a genetic variability and populations’ structure study is performed studying 21 geographical populations of G. dissectum. Perhaps the most important biological challenge today is the conservation of biodiversity. Populations were studied from morphological and genetic (ISSR) points of views. Both intra and inter-population morphological and genetic variability was observed in the studied accessions. ANOVA and CVA tests revealed significant morphological difference among populations. Similarly, AMOVA and Hickory tests revealed significant molecular difference among geographical populations. Mantel test produced significant positive correlation between genetic and geographical distance of the studied populations. Networking, STRUCTURE analyses and population assignment test revealed some degree of gene flow among these populations. Consensus tree based on morphological and genetic data separated some of these populations from the others suggesting the existence of ecotypes within this species.
Acknowledgements
I would like to thank Mokhtar Hasanzadeh for his assistance in collection of plant from the wild.
References
Azizi N., Sheidai M., Mozafarian V. & Noormohammadi Z. 2014. Genetic, cytogenetic and morphological diversity in Helicrysum leucocephalum (Asteraceae) populations. Biologia 69: 566–573.10.2478/s11756-014-0352-1Suche in Google Scholar
Alves M.C., Leitaõ M.T. 1976. Contribuca̹o para o conhecimento citotaxonomico des Spermatophyta de Portugal XIII. Boletim da Sociedade Broteriana serie 2, 50: 231–245.Suche in Google Scholar
Aedo C. 1996. Revision of Geranium subgenus Erodioidea (Geraniaceae). Syst. Bot. Monogr. 49: 1–104.10.2307/25027866Suche in Google Scholar
Aedo C.F., Muñoz Garmendia F. & Pando F. 1998a. World checklist of Geranium L. (Geraniaceae). Anal. Jardín Bot. Madrid 56: 211–252.10.3989/ajbm.1998.v56.i2.230Suche in Google Scholar
Aedo C., Aldasoro J.J. & Navarro C. 1998b. Taxonomic revision of Geranium L., sections Divaricata Rouy and Batrachioidea W.D.J. Koch (Geraniaceae). Ann. Miss. Bot. Garden 85: 594–630.10.2307/2992018Suche in Google Scholar
Aedo C., Fiz O., Alarcó M., Navarro C. & Aldasoro J. 2005.Taxonomic Revision of Geranium sect. Dissecta (Geraniaceae). System. Bot. 30: 533–55810.1600/0363644054782260Suche in Google Scholar
Chen X.Y. 2000. Effects of fragmentation on genetic structure of plant populations and implications for the biodiversity conservation. Acta Ecol. Sin. 20: 884–892.Suche in Google Scholar
Calzada F., Cervantes-Martinez J.A. & Yepez-Mulia L. 2005. In vitro antiprotozoal activity from the roots of Geranium mexicanum and its constituents on Entamoeba histolytica and Giardia lamblia. J. Ethnopharmacol. 98: 191–193.10.1016/j.jep.2005.01.019Suche in Google Scholar PubMed
Davis P.H. 1965. Geraniaceae, pp. 450–488. In: Davis P.H. (ed.), Flora of Turkey and East Aegean Islands 2.Suche in Google Scholar
Ellis J.R. & Burke J.M. 2007. EST-SSRs as a resource for population genetic analyses. Heredity 99: 125–32.10.1038/sj.hdy.6801001Suche in Google Scholar PubMed
De Leonardis W., Pavone P., Terrasi M.C. & Zizza A.1981. Numeri cromosomici per la Flora Italiana: 814–830. Inf. Botanico Italiano 13: 158–167.Suche in Google Scholar
Esfandani Bozchaloyi S., Sheidai M., Keshavarzi M. & Noormohammadi Z. 2017a. Genetic Diversity and Morphological Variability in Geranium purpureum Vill. (Geraniaceae) of Iran. Genetika 49: 543–557.10.2298/GENSR1702543BSuche in Google Scholar
Esfandani Bozchaloyi S., Sheidai M., Keshavarzi M. & Noormohammadi Z. 2017b. Species Delimitation in Geranium Sect. Batrachioidea: Morphological and Molecular. Acta Bot. Hung. 59(3-4): 10.1556/034.59.2017.3-4.3Suche in Google Scholar
Evanno G., Regnaut S. & Goudet J. 2005. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol. Ecol. 14: 2611–2620.10.1111/j.1365-294X.2005.02553.xSuche in Google Scholar PubMed
Falush D., Stephens M. & Pritchard J.K. 2007. Inference of population structure using multilocus genotype data: dominant markers and null alleles. Mol. Ecol. Notes7: 574–578.10.1111/j.1471-8286.2007.01758.xSuche in Google Scholar PubMed PubMed Central
Freeland J.R., Kirk H. & Peterson S.D. 2011. Molecular Ecology (2nd ed). Wiley-Blackwell, UK, 449 pp.10.1002/9780470979365Suche in Google Scholar
Huson D.H., Bryant D. 2006. Application of phylogenetic networks in evolutionary studies. Mol. Biol. Evol. 23: 254–267.10.1093/molbev/msj030Suche in Google Scholar PubMed
Gauger W. 1937. Ergebnisse einer zytologischen Untersuchung der Familie der Geraniaceae. I. Planta. 26: 529–531.10.1007/BF01914322Suche in Google Scholar
Gadella T.W.J. & Kliphuis E.1966. Chromosome numbers of flowering plants in the Netherlands II. Proc. of the Koninklijke Nederlandse Akademie van Wetenschappen: Series C 69: 541–556.Suche in Google Scholar
Hamer O., Harper D.A. & Ryan P.D. 2012. PAST: Paleontological Statistics software package for education and data analysis. Palaeonto. Electro. 4: 9.Suche in Google Scholar
Hedrick P. W. 2005. A standardized genetic differentiation measure. Evolution 59: 1633–1638.10.1111/j.0014-3820.2005.tb01814.xSuche in Google Scholar PubMed
Jost L. 2008. GST and its relatives do not measure differentiation. Mol. Ecol. 17: 4015–4026.10.1111/j.1365-294X.2008.03887.xSuche in Google Scholar
Jolivet C. & Bernasconi G. 2007. Within/between population crosses reveal genetic basis for pollen competitive ability in Silene latifolia (Caryophyllaceae). J. Evol. Biol. 20: 1361–1374.10.1111/j.1420-9101.2007.01344.xSuche in Google Scholar PubMed
Janighorban M. 2009. Flora of Iran. Geraniaceae. Vol. 62. The Research Institute of Forests and Rangelands. [in Persian]Suche in Google Scholar
Khadivi-Khub A., Aghaei Y. & Mirjalili MH. 2014. Phenotypic and phytochemical diversity among different populations of St. lavandulifolia. Biochem Syst Ecol. 54: 272–8.10.1016/j.bse.2014.02.024Suche in Google Scholar
Kharazian N., Rahimi S. & Shiran B. 2015. Genetic diversity and morphological variability of fifteen Stachys (Lamiaceae) species from Iran using morphological and ISSR molecular markers. Biologia 70: 438–52.10.1515/biolog-2015-0051Suche in Google Scholar
Löve A. 1945. Cyto-taxonomical studies on boreal plants. III. Some new chromosome numbers of Scandinavian plants. Arkiv for Botanik 31A (12): 1–22.Suche in Google Scholar
Löve, A. & Kjellqvist E. 1974. Cytotaxonomy of Spanish plants. IV. Dicotyledons: Caesalpinaceae-Asteraceae. Lagascalia 4: 153–211.Suche in Google Scholar
Meirmans P.G. & Van Tienderen P.H. 2004. GENOTYPE and GENODIVE: two programs for the analysis of genetic diversity of asexual organisms. Mol. Ecol. Notes4: 792–794.10.1111/j.1471-8286.2004.00770.xSuche in Google Scholar
Meirmans P.G. 2012. AMOVA-based clustering of population genetic data. J. Heredity 103: 744–750.10.1093/jhered/ess047Suche in Google Scholar PubMed
Minaeifar A.A., Sheidai M., Attar F. & Noormohammadi Z., Ghasemzadeh-Baraki B. 2015. Genetic and morphological diversity in Cousinia tabrisiana (Asteraceae) populations. Biologia 70: 328–38.10.1515/biolog-2015-0042Suche in Google Scholar
Májovský J. (ed.). 1974. Index of chromosome numbers of Slovakian flora (Part 3). Acta Fac. Rerum Nat. Univ. Com., Botanica 22: 1–20.Suche in Google Scholar
Mabberley D.J. 2008. Mabberley’s Plant Book. A portable dictionary of plants, their classification and uses. Cambridge: Cambridge University Press.Suche in Google Scholar
Onsori S., Salimpour F. 7 Mazooji A. 2010. The new record of Geranium linearilobum Dc. based on anatomy and micromorphological study of pollen and seed, in Iran. J. Plant Sci. Res. 5: 21–30. (in Persian with English abstract)Suche in Google Scholar
Peakall R. & Smouse P.E. 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol. Ecol. Notes 6: 288–295.10.1111/j.1471-8286.2005.01155.xSuche in Google Scholar
Podani J. 2000. Introduction to the Exploration of Multivariate Data English translation. Backhuyes publisher, Leide, 407 pp.Suche in Google Scholar
Pritchard J.K., Stephens M. & Donnelly P. 2000. Inference of population structure using multilocus genotype data. Genetics 155: 945–959.10.1093/genetics/155.2.945Suche in Google Scholar PubMed PubMed Central
Rezakhanlo A. & Talebi S.M. 2010, Trichomes morphology of stachys lavandulifolia vahl. (Labiatae) of Iran. Proc. Soc. Behav. Sci.2: 3755–376310.1016/j.sbspro.2010.03.584Suche in Google Scholar
Sheidai M., Ahmad-Khanbeygi M. & Attar F. 2012.New chromosome number reports in Cousinia species(Compositae). Cytologia 77: 11–16.10.1508/cytologia.77.11Suche in Google Scholar
Sheidai M., Zanganeh S., Haji-Ramezanali R., Nouroozi M., Noormohammadi Z. & Ghsemzadeh-Baraki S. 2013. Genetic diversity and population structure in four Cirsium (Asteraceae) species. Biologia 68: 384–397.10.2478/s11756-013-0162-xSuche in Google Scholar
Sheidai M., Afshar F., Keshavarzi M., Talebi SM., Noormohammadi Z. & Shafaf T. 2014.Genetic diversity and genome size variability in Linum austriacum (Linaceae) populations. Biochem. Syst. Ecol. 57: 20–26.10.1016/j.bse.2014.07.014Suche in Google Scholar
Schönbeck-Temesy E. 1970. Geraniaceae, pp. 30–58. In: Rechinger K.H. (ed.), Flora Iranica, Vol 69, Akademische Druck, Graz, Austria.Suche in Google Scholar
Weising K., Nybom H., Wolff K. & Kahl G. 2005. DNA Fingerprinting in Plants. Principles, Methods, and Applications. 2nd ed. CRC Press, Boca Rayton, 472 pp.10.1201/9781420040043Suche in Google Scholar
Warburg E.F. 1938. Taxonomy and relationship in the Geraniales in the light of their cytology. New Phytol. 37: 130–159.10.1111/j.1469-8137.1938.tb06932.xSuche in Google Scholar
Yeo P.F. 1984. Fruit-discharge-type in Geranium (Geraniaceae): its use in classification and its evolutionary implications. Bot. J. Linn. Soc. 89: 1–36.10.1111/j.1095-8339.1984.tb00998.xSuche in Google Scholar
Zohary M. 1972. Flora Palaestina. Platanaceae to Umbelliferae. The Israel Academy of Sciences and Humanities, Jerusalem, Israel.Suche in Google Scholar
© 2017 Institute of Botany, Slovak Academy of Sciences
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- Cellular and Molecular Biology
- Therapeutic strategies to fight HIV-1 latency: progress and challenges
- Zoology
- Detection of schistosomiasis applicable for primary health care facilities in endemic regions of Africa
- Botany
- Genetic and morphological diversity in Geranium dissectum (Sec. Dissecta, Geraniaceae) populations
- Botany
- Optical properties of halophyte leaves are affected by the presence of salt on the leaf surface
- Botany
- The crosstalk between ABA, nitric oxide, hydrogen peroxide, and calcium in stomatal closing of Arabidopsis thaliana
- Botany
- Optimization of the pollen-tube pathway method of plant transformation using the Yellow Cameleon 3.6 calcium sensor in Solanum lycopersicum
- Cellular and Molecular Biology
- Differential effects of plant growth regulators on physiology, steviol glycosides content, and antioxidant capacity in micropropagated tissues of Stevia rebaudiana
- Zoology
- Characteristics of matrix of the invasive freshwater Ectoprocta species Pectinatella magnifica
- Zoology
- Relative growth and reproductive cycle of the hermaphroditic Cardites antiquatus (Mollusca: Bivalvia) collected from the Bizerte channel (northern Tunisia)
- Zoology
- Tegolophus glycyglabri sp. n. (Trombidiformes: Eriophyidae), a new species from Iran
- Zoology
- Karyotype characteristics and polymorphism peculiarities of Chironomus luridus (Diptera: Chironomidae) from Central and Northwest Caucasus
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- Reptile surveys reveal high species richness in areas recovering from mining activity in the Brazilian Cerrado
- Zoology
- The porcupine as “Little Thumbling”: The role of Hystrix cristata in the spread of Helianthus tuberosus
- Cellular and Molecular Biology
- Distribution of telocytes in the corpus and cervix of human uterus: an immunohistochemical study
- Zoology
- First record of mermithid larva (Nematoda: Mermithidae) in Anopheles maculipennis complex (Diptera: Culicidae) imago in Central-Europe