The evaluation of endocrine regulators after intramuscular and oral application of cyanogenic glycoside amygdalin in rabbits
-
Marek Halenar
,
Lubica Chrastinova
Abstract
The present in vivo study was designed to reveal whether amygdalin is able to cause changes in the endocrine profile and thus alter the key reproductive and physiological functions, using rabbits as a biological model. 40 adult female rabbits were randomly divided into five groups: the control group without any amygdalin administration, two experimental groups received a daily intramuscular injection of amygdalin at dose 0.6 and 3.0 mg kg-1 b.w. and other two groups were fed by crushed apricot seeds at dose 60 and 300 mg kg-1 b.w., mixed with commercial feed over the period of 28 days. The body weight of each experimental animal was recorded weekly during the whole study. Plasma levels of steroid (progesterone, 17β-estradiol, testosterone), thyroid (triiodothyronine, thyroxine, thyroid-stimulating hormone), as well as anterior pituitary (prolactin, luteinizing hormone) hormones were assessed by ELISA. Intramuscular application of amygdalin did not affect (P ≥ 0.05) the plasma levels of none of the endocrine regulators selected. Oral form of amygdalin did not induce significant changes in the plasma levels of examined hormones either. Similarly, no clinically noticeable changes in the average body weight of experimental animals were observed. Our findings indicate that intramuscular and oral application of amygdalin did not significantly affect the plasma levels of studied endocrine regulators in experimental rabbits. In this in vivo study, no obvious beneficial or negative effects of amygdalin on the physiological functions of female rabbits were demonstrated. Short-term intake of amygdalin at the recommended doses does not represent risk for animal health.
Acknowledgements
This work was financially supported by the Ministry of Education, Science, Research and Sport of the Slovak Republic projects no. 1/0039/16, 011SPU-4/2016 KEGA, APVV-0304-12, and European Community under project no. 26220220180: Building Research Centre “AgroBioTech”.
Declaration of interest: The authors have reported that no competing interests exist.
References
Ames M.M., Moyer T.P., Kovach J.S., Moertel C.G. & Rubin J. 1981. Pharmacology of amygdalin (Laetrile) in cancer patients. Cancer Chemother. Pharmacol. 6: 51–57. DOI: 10.1007/BF00253010Suche in Google Scholar
Balazi A., Foldesiova M., Chrastinova L., Sirotkin A.V. & Chrenek P. 2013. The effect of herbal plants Yucca, schidigera, and Curcuma longa on rabbit female reproduction. Slovak J. Anim. Sci. 46: 161–171.Suche in Google Scholar
Balmer C. 1998. Alternative therapies in cancer patient care. Highlight. Oncol. Pract. 15: 83–84.Suche in Google Scholar
Chang H.K., Shin M.S., Yang H.Y., Lee J.W., Kim Y.S., Lee M.H., Kim J., Kim K.H. & Kim C.J. 2006. Amygdalin induces apoptosis through regulation of Bax and Bcl-2 expressions in human DU145 and LNCaP prostate cancer cells. Biol. Pharm. Bull. 29: 1597–1602. DOI: 10.1248/bpb.29.1597Suche in Google Scholar
Chedrese J.P. (ed.). 2009. Reproductive Endocrinology: A Molecular Approach. Springer Science+Business Media LLC., New York, 361 pp. ISBN: 978-0-387-88186-7Suche in Google Scholar
Chow H.H.S., Garland L.L., Heckman-Stoddard B.M., Hsu C.H., Butler V.D., Cordova C.A., Chew W.M. & Cornelison T.L. 2014. A pilot clinical study of resveratrol in postmenopausal women with high body mass index: effects on systemic sex steroid hormones. J. Transl. Med. 12: 223. DOI: 10.1186/s12967-014-0223-0Suche in Google Scholar
EFSA CONTAM Panel (EFSA Panel on Contaminants in the Food Chain). 2016. Acute health risks related to the presence of cyanogenic glycosides in raw apricot kernels and products derived from raw apricot kernels. EFSA J. 14 (4): 4424. DOI: 10.2903/j.efsa.2016.4424Suche in Google Scholar
FAO/WHO (Food and Agricultural Organization/World Health Organization). 2012. Safety evaluation of certain food additives and contaminants. Prepared by the seventy-fourth meeting of the joint FAO/WHO expert committee on food additives (JECFA). WHO Food Additives Series 65: 1–825. ISBN: 978-92-4-166065-5Suche in Google Scholar
Fukuda T., Ito H., Mukainaka T., Tokuda H., Nishino H. & Yoshida T. 2003. Anti-tumor promoting effect of glycosides from Prunus persica seeds. Biol. Pharma. Bull. 26: 271–273. PMID: 1257669310.1248/bpb.26.271Suche in Google Scholar PubMed
Galik B., Arpasova H., Biro D., Rolinec M., Simko M., Juracek M. & Herkel R. 2014. The effect of dietary Rhus coriaria L. on table eggs yolk nutrients composition. Acta Fytotechn. Zootechn. 17 (3): 93–95. DOI: 10.15414/afz.2014.17.03.93-95Suche in Google Scholar
Galik B., Biro D., Juracek M., Simko M. & Gyongyova E. 2009. Koncentracije mineralnih elemenata u različitim konzerviranim krmivima. The concentration of mineral elements in different conserved feeds. Krmiva: Časopis o hranidbi životinja proizvodnji i tehnologiji krme [Krmiva: Review for Animal Feeding, Pproduction and Feed Technology] (Zagreb) 51 (4): 223–227.Suche in Google Scholar
Halenar M., Medvedova M., Maruniakova N. & Kolesarova A. 2015. Assessment of a potential preventive ability of amygdalin in mycotoxin-induced ovarian toxicity. J. Environ. Sci. Health. 50: 411–416. DOI: 10.1080/03601234.2015.1011956Suche in Google Scholar
Huebner J., Micke O., Muecke R., Buentzel J., Prott F.J., Kleeberg U., Senf B. & Muenstedt K. 2014. User rate of complementary and alternative medicine (CAM) of patients visiting a counseling facility for CAM of a German comprehensive cancer center. Anticancer Res. 34: 943–948. PMID: 24511037Suche in Google Scholar
Hwang H.J., Lee H.J., Kim CH.J., Shim I. & Hahm D.H. 2008. Inhibitory effect of amygdalin on lipopolyccharide-inducible TNF-α and IL-1β mRNA expression and carrageenan-induced rats arthritis. J. Microbiol. Biotechnol. 18: 1641–1647. PMID: 18955812Suche in Google Scholar
Igarashi M. 1988. Kampo medicine in endocrinology, pp. 157–160. In: Hosoya E. & Yamamura Y. (eds), Recent Advances in the Pharmacology of Kampo (Japanese herbal) Medicines: Proceedings of the Satellite Meeting on Kampo (Japanese Herbal) Medicines of the 10th International Congress of Pharmacology, Auckland, August 19–21, Excerpta Medica, Tokyo, 470 pp. ISBN: 0444810641, 9780444810649Suche in Google Scholar
Kolesarova A., Capcarova M., Bakova Z., Galik B., Juracek M., Simko M. & Sirotkin A.V. 2011. The effect of bee pollen on secretion activity, markers of proliferation and apoptosis of porcine ovarian granulosa cells in vitro. J. Environ. Sci. Health. 46: 207–212. DOI: 10.1080/03601234.2011.540202Suche in Google Scholar
Kolesarova A., Capcarova M., Maruniakova N., Lukac N., Ciereszko R. & Sirotkin A. V. 2012. Resveratrol inhibits reproductive toxicity induced by Deoxynivalenol. J. Environ. Sci. Health. 47: 1329–134. DOI: 10.1080/10934529.2012.672144Suche in Google Scholar
Kolesarova A., Roychoudhury S., Slivkova J., Sirotkin A.V., Capcarova M. & Massanyi P. 2010. In vitro study on the effects of lead and mercury on porcine ovarian granulosa cells. J. Environ. Sci. Health. 45: 320–331. DOI: 10.1080/10934520903467907Suche in Google Scholar
Londonkar R.L. & Nayaka H.B. 2013. Effect of ethanol extract of Portulaca oleracea L on ovulation and estrous cycle in female albino rats. J. Pharma. Res. 6: 431–436. DOI: 10.1016/j.jopr.2013.02.030Suche in Google Scholar
Makarević J., Rutz J., Juengel E., Kaulfuss S., Reiter M., Tsaur I., Bartsch G., Haferkamp A. & Blaheta R.A. 2014. Amygdalin blocks bladder cancer cell growth in vitro by diminishing cyklin A and cdk2. PLoS One 9: e105590. DOI: 10.1371/journal.pone.0105590Suche in Google Scholar
Milazzo S., Lejeune S. & Ernst E. 2007. Laetrile for cancer: a systematic review of the clinical evidence. Support Care Cancer. 15: 583–595. DOI: 10.1007/s00520-006-0168-9Suche in Google Scholar
Moertel C.G., Ames M.M., Kovach J.S., Moyer T.P., Rubin J.R. & Tinker J.H. 1981. A pharmacologic and toxicological study of amygdalin. J. Am. Med. Assoc. 245: 591–594. DOI: 10.1001/jama.245.6.591Suche in Google Scholar
Medvedova M., Kolesarova A., Capcarova M., Labuda R., Sirotkin A.V., Kovacik J. & Bulla J. 2011. The effect of deoxynivalenol on the secretion activity, proliferation and apoptosis of porcine ovarian granulosa cells in vitro. J. Environ. Sci. Health. 46: 213–219. DOI: 10.1080/03601234.2011.540205Suche in Google Scholar
Nelson K.W. 1998. Alternative cancer treatments. Highlights in Oncology Practice 15 (4): 85–93.Suche in Google Scholar
Ranzenigo G., Caloni F., Cremonesi F., Aad P.Y. & Spicer L.J. 2008. Effects of Fusarium mycotoxins on steroid production by porcine granulosa cells. Anim. Reprod. Sci. 107: 115–130. DOI: 10.1016/j.anireprosci.2007.06.023Suche in Google Scholar
Saalu L.C., Akunna G.G. & Ajayi J.O. 2013. Modulating role of bitter leaf on spermatogenic and steroidogenesis functions of the rat testis. Am. J. Biochem. Mol. Biol. 3: 314–321. DOI: 10.3923/ajbmb.2013.314.321Suche in Google Scholar
Sakarkar D.N. & Deshmukh V.N. 2011. Ethnopharmacological review of traditional medicinal plants for anticancer activity. Int. J. PharmTech. Res. 3: 298–308.Suche in Google Scholar
Usuki S. 1987. Effects of Hachimijiogan, Tokishakuyakusan and Keishibukuryogan on progesterone secretions by corpus luteum. Am. J. Chin. Med. 15: 109. DOI: 10.1142/S0192415X 8700014XSuche in Google Scholar
Usuki S. 1990. Effects of Tokishakuyakusan and Keishibukuryogan on steroidogenesis by rat preovulatory follicles in vivo. Am. J. Chin. Med. 18 (3–4): 149–156. DOI: 10.1142/S0192 415X90000198Suche in Google Scholar
Usuki S. 1991. Effects of Hachimijiogan, Tokishakuyakusan and Keishibukuryogan, Ninjinto and Unkeito on estrogen and progesterone secretion in preovulatory follicles incubated in vitro. Am. J. Chin. Med. 19: 65–71. DOI: 10.1142/S0192415X91000107Suche in Google Scholar
Yan J., Tong S., Li J. & Lou J. 2006. Preparative isolation and purification of amygdalin from Prunus armeniaca L. with high recovery by high-speed countercurrent chromatography. J. Liq. Chrom. Rel. Tech. 29: 1271–1279. DOI: 10.1080/10826070600598985Suche in Google Scholar
Yasui T., Matsuzaki T., Ushigoe K., Kuwahara A., Maegawa M., Furumoto H., Aono T. & Irahara M. 2003. Stimulatory effect of the herbal medicine Keishi-bukuryo-gan on a cytokine-induced neutrophil chemoattractant, in rat ovarian cell culture. Am. J. Reprod. Immunol. 50: 90–97. DOI: 10.1034/j.1600-0897.2003.00055.xSuche in Google Scholar
Yildirim F.A. & Askin M.A. 2010. Variability of amygdalin content in seeds of sweet or bitter apricot cultivars in Turkey. Afr. J. Biotech. 9 (39): 6522–6524. DOI: 10.5897/AJB10.884Suche in Google Scholar
Zhou C., Qian L., Ma H., Yu X., Zhang Y., Qu W., Zhang X. & Xiam W. 2012. Enhancement of amygdalin activated with β-D glucosidase on HepG2 cells proliferation and apoptosis. Carbohydr. Polym. 90: 516–523. DOI: 10.1016/j.carbpol.2012.05.073Suche in Google Scholar
© 2017 Institute of Zoology, Slovak Academy of Sciences
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- Celluar and Molecular Biology
- Occurrence of Anaplasma phagocytophilum in three sympatric tick species in the South Moravia, Czech Republic
- Botany
- Germination strategies of two dominant Carex species in a swamp alder forest: implications for restoration
- Botany
- Role of ethylene and phospholipid-mediated signalling in mycotoxin-induced programmed cell death in the apical part of maize roots
- Botany
- Transcriptome analysis in leaves of rice (Oryza sativa) under high manganese stress
- Botany
- Type II metacaspase protein localization and gene transcription during programmed cell semi-death of sieve elements in developing caryopsis of Tritium aestivum
- Zoology
- Environmental factors determining the distribution pattern of leeches (Clitellata: Hirudinida) in large river systems: A case study of the Oder River system
- Zoology
- Effects of beaver dams on the zooplankton assemblages in four temperate lowland streams (NW Poland)
- Zoology
- Bioassessment of streams based on macroinvertebrates — can sampling of some substrate types be excluded?
- Zoology
- Communities of oribatid mites (Acari: Oribatida) of naturally regenerating and salvage-logged montane spruce forests of Šumava Mountains
- Zoology
- Food digestibility and consumption rate in detrito-bryophagous groundhopper Tetrix subulata (Orthoptera: Tetrigidae)
- Zoology
- Dietary alpha-ketoglutarate partially prevents age-related decline in locomotor activity and cold tolerance in Drosophila melanogaster
- Zoology
- The evaluation of endocrine regulators after intramuscular and oral application of cyanogenic glycoside amygdalin in rabbits
