Startseite Lebenswissenschaften Dietary alpha-ketoglutarate partially prevents age-related decline in locomotor activity and cold tolerance in Drosophila melanogaster
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Dietary alpha-ketoglutarate partially prevents age-related decline in locomotor activity and cold tolerance in Drosophila melanogaster

  • Maria M. Bayliak EMAIL logo , Maria P. Lylyk und Oksana M. Sorochynska
Veröffentlicht/Copyright: 28. April 2017
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Biologia
Aus der Zeitschrift Biologia Band 72 Heft 4

Abstract

Alpha-ketoglutarate (AKG), a key intermediate in the Krebs cycle and metabolism of amino acids, is one of dietary supplements studied actively during last decade. In this work, we examined the ability of dietary AKG to prevent age-related functional decline in D. melanogaster. For that, some metabolic processes and physiological functions, such as climbing activity and cold resistance, were studied in young (2 days post-eclosion) and middle-aged (24 days) D. melanogaster w1118 adults maintained on AKG-supplemented diet. Both locomotor activity and cold resistance measured as chill coma recovery time (CCRT) impaired in middle-aged flies compared with young ones. Diet with 10 mM AKG improved climbing activity in middle-aged flies of both sexes. A positive impact of AKG on CCRT was found in young flies of both sexes and in middle-aged females. AKG-containing diet did not affect cold hardening in young flies, whereas middle-aged flies fed on AKG were more resistant to repeated cold treatments than control ones. Two-day old flies reared on AKG had higher free amino acid level, including that of proline. At 24-day age, AKG-fed females but not males had higher levels of free amino acids and glucose, and higher total antioxidant capacity than control ones. We conclude that both the enhancement of antioxidant capacity and synthesis of amino acids can contribute to AKG-promoted reduction in CCRT. However, since dietary AKG enhanced locomotor activity and resistance to repeated cold stresses in middle-aged both males and females, mechanisms of beneficial effects of AKG seem to be more complicated, that is discussed.

Key words: amino acids; total antioxidant capacity; glucose; functional senescence; stress resistance

Acknowledgements

We are grateful to Bloomington Stock Center (Indiana University, USA) for providing D. melanogaster strain, Ms. U. Dzaman for English editing of the manuscript, O. Semchyshyn, O. Manyuch, and H. Shmihel for technical assistance.

  1. Conflict of interest: The authors have declared that there is no conflict of interest.

Abbreviations

ABTS

2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

AKG

alpha-ketoglutarate

CCRT

chill coma recovery time

ROS

reactive oxygen species

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Received: 2016-10-12
Accepted: 2017-1-25
Published Online: 2017-4-28
Published in Print: 2017-4-25

© 2017 Institute of Zoology, Slovak Academy of Sciences

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https://doi.org/10.1515/biolog-2017-0042
Schlagwörter für diesen Artikel
amino acids; total antioxidant capacity; glucose; functional senescence; stress resistance

Artikel in diesem Heft

  1. Celluar and Molecular Biology
  2. Occurrence of Anaplasma phagocytophilum in three sympatric tick species in the South Moravia, Czech Republic
  3. Botany
  4. Germination strategies of two dominant Carex species in a swamp alder forest: implications for restoration
  5. Botany
  6. Role of ethylene and phospholipid-mediated signalling in mycotoxin-induced programmed cell death in the apical part of maize roots
  7. Botany
  8. Transcriptome analysis in leaves of rice (Oryza sativa) under high manganese stress
  9. Botany
  10. Type II metacaspase protein localization and gene transcription during programmed cell semi-death of sieve elements in developing caryopsis of Tritium aestivum
  11. Zoology
  12. Environmental factors determining the distribution pattern of leeches (Clitellata: Hirudinida) in large river systems: A case study of the Oder River system
  13. Zoology
  14. Effects of beaver dams on the zooplankton assemblages in four temperate lowland streams (NW Poland)
  15. Zoology
  16. Bioassessment of streams based on macroinvertebrates — can sampling of some substrate types be excluded?
  17. Zoology
  18. Communities of oribatid mites (Acari: Oribatida) of naturally regenerating and salvage-logged montane spruce forests of Šumava Mountains
  19. Zoology
  20. Food digestibility and consumption rate in detrito-bryophagous groundhopper Tetrix subulata (Orthoptera: Tetrigidae)
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  22. Dietary alpha-ketoglutarate partially prevents age-related decline in locomotor activity and cold tolerance in Drosophila melanogaster
  23. Zoology
  24. The evaluation of endocrine regulators after intramuscular and oral application of cyanogenic glycoside amygdalin in rabbits
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