Abstract
Germination strategy is an essential mechanism that determines plant survival in previously established populations or newly colonised sites. Carex is a group of species that has shown difficulties to germinate experimentally and also many of them failed in order to use in restoration projects. Our aim was to determine whether Carex elata and C. elongata that dominate in vegetation of Central European swamps differ in their germination strategy. We conducted germination experiments with stratified and unstratified seeds of both species to determine: 1) if they are able to germinate fresh, 2) if they exhibit a cyclic dormancy pattern, and 3) if they will germinate from a seed bank. We demonstrate fresh seed germination and no evidence of cyclic dormancy in either species. Stratification did not enhance final germination but it did accelerate germination. Seed bank seeds of both species germinate sparsely. We demonstrate that these coexisting Carex species differ with respect to final germination. The higher germination percentages of the fresh seeds compared to buried and seed bank seeds of both species probably reflect adaptation to fluctuating water-level conditions. In summary, these findings support a strategy of fresh germination in a highly-variable environment. Our study indicates that both C. elata and C. elongata are suitable for restoration projects. Successful establishment and revegetation with C. elongata may result simply from sowing fresh seeds. In contrast, seed sowing, combined with vegetatively produced seedling transplants is essential for the successful restoration of C. elata.
Acknowledgements
This study was supported by the Internal Grant Agency of the Faculty of Environmental Sciences, CULS Prague, 2014, grant no. 4211013123129. We would like to thank Aneta Kyselova for help with the germination experiment.
References
Angevine M.W. & Chabot B.F. 1979. Seed germination syndromes in higher plants, pp. 188–206. In: Solbrig O.T., Jain S., Johnson G.B. & Raven P.H. (eds), Topics in Plant Population Biology, Columbia University Press, New York.10.1007/978-1-349-04627-0_9Search in Google Scholar
Baskin C.C. & Baskin J.M. 1988. Germination ecophysiology of herbaceous plant species in a temperate region. Am. J. Bot. 75: 286-305.10.1002/j.1537-2197.1988.tb13441.xSearch in Google Scholar
Baskin C.C. & Baskin J.M. 1993. Seed germination ecophysiology of four summer annual mudflat species of Cyperaceae. Aquat. Bot. 45: 41-52.10.1016/0304-3770(93)90051-WSearch in Google Scholar
Baskin C.C. & Baskin J.M. 1998. Seeds. Ecology, Biogeography, and Evolution of Dormancy and Germination. Academic Press, San Diego, 665 pp.Search in Google Scholar
Baskin C.C, Chesson P.L. & Baskin J.M. 1993. Annual seed dormancy cycles in two desert winter annuals. J. Ecol. 81: 551-556.10.2307/2261533Search in Google Scholar
Baskin C.C, Chester W.E. & Baskin J.M. 1996. Effect of flooding on annual dormancy cycles in buried seeds of two wetland Carex species. Wetlands 16: 84–88.10.1007/BF03160648Search in Google Scholar
Brandel M. 2005. The effect of stratification temperatures on the level of dormancy in primary and secondary dormant seeds of two Carex species. Plant Ecol. 178: 163–169.10.1007/s11258-004-3037-ySearch in Google Scholar
Budelsky R.A. & Galatowitsch S.M. 1999. Effects of moisture, temperature, and time on seed germination of five wetland Carices: implications for restoration. Restor. Ecol. 7: 86–97.10.1046/j.1526-100X.1999.07110.xSearch in Google Scholar
Cochrane A., Kelly A., Brown K. & Cunneen S. 2002. Relationships between seed germination requirements and ecophysiological characteristics aid the recovery of threatened native plant species in Western Australia. Ecol. Manage. Restor. 3: 47-60.10.1046/j.1442-8903.2002.00089.xSearch in Google Scholar
Core Team R. 2015. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria, URL http://www.R-project.org.Search in Google Scholar
Crawley M. J. 2012. The R book, 2nd edition. John Wiley & Sons, Inc., Chichester, 1076 pp.Search in Google Scholar
Czerepko J., Boczon A., Pierzgalski E., Sokolowski A.W. & Wróbel M. 2007. Habitat diversity and spontaneous succession of forest wetlands in Bialowieza primeval forest, pp. 37–43. In: Okruszko T., Maltby E., Szatylowicz J., Miroslaw-Swiatek D. & Kotowski W. (eds), Wetlands: Modeling, Monitoring and Management, Taylor and Francis, London.Search in Google Scholar
Douda J., Boublík K., Slezák M., Biurrun I., Nociar J., Havrdová A., Doudová J., Aćić S., Brisse H., Brunet J., Chytrý M., Claessens H., Csiky J., Didukh Y., Dimopoulos P., Dullinger S., FitzPatrick Ú., Guisan A., Horchler P.J., Hrivnák R., Jandt U., Kącki Z., Kevey B., Landucci F., Lecomte H., Lenoir J., Paal J., Paternoster D., Pauli H., Pielech R., Rodwell J.S., Roelandt B., Svenning J.C., Šibík J., Šilc U., Škvorc Ž., Tsiripidis I., Tzonev R.T., Wohlgemuth T. & Zimmermann N.E. 2016a. Vegetation classification and biogeography of European floodplain forests and alder carrs. Appl. Veg. Sci. 19: 147-163.10.1111/avsc.12201Search in Google Scholar
Douda J., Čejková A., Douda K. & Kochánková J. 2009. Development of alder carr after the abandonment of wet grasslands during the last 70 years. Ann. For. Sci. 66: 1–13.10.1051/forest/2009065Search in Google Scholar
Douda J., Doudová-Kochánková J., Boublik K. & Drašnarová A. 2012. Plant species coexistence at local scale in temperate swamp forest: test of habitat heterogeneity hypothesis. Oecologia 169: 523-534.10.1007/s00442-011-2211-xSearch in Google Scholar PubMed
Douda J., Hulík J. & Doudová J. 2016b. Vegetative sprouting as an additional pathway for a seed size-number trade-off: a field-parameterised simulation approach. Community Ecol. 17: 205-215.10.1556/168.2016.17.2.9Search in Google Scholar
Emrani S.N., Arzani A. & Saeidi G. 2013. Seed viability, germination and seedling growth of canola (Brassica napus L.) as influenced by chemical mutagens. Afr. J. Biotechnol. 10: 12602-12613.Search in Google Scholar
Eriksson O. & Fröborg H. 1996. “Windows of opportunity” for recruitment in long-lived clonal plants: experimental studies of seedling establishment in Vaccinium shrubs. Can. J. Bot. 74: 1369-1374.10.1139/b96-166Search in Google Scholar
Fernández-Pascual E., Jiménez-Alfaro B. & Díaz T.E. 2013. The temperature dimension of the seed germination niche in fen wetlands. Plant ecol. 214: 489-499.10.1007/s11258-012-0165-7Search in Google Scholar
Fojt W. & Harding M. 1995. Thirty years of change in the vegetation communities of valley mires in Suffolk, England. J. Appl. Ecol. 32: 561-577.10.2307/2404653Search in Google Scholar
Grime J.P., Mason G., Curtis A.V., Rodman J., Band S.R., Mowforth M.A.G., Neal A.M. & Shaw S. 1981. A comparative study of germination characteristics in a local flora. J. Ecol. 69: 1017-1059.10.2307/2259651Search in Google Scholar
Gross N., Suding K.N., Lavorel S. & Roumet C. 2007. Complementarity as a mechanism of coexistence between functional groups of grasses. J. Ecol. 95: 1296–1305.10.1111/j.1365-2745.2007.01303.xSearch in Google Scholar
Hegi G. 1980. Illustrierte Flora von Mitteleuropa, Band 2, Teil 1, Cyperaceae, Typhaceae incl. Sparganiaceae, Araceae, Lemnaceae, Juncaceae, 3rd edition. Parey, Berlin.Search in Google Scholar
Hothorn T., Bretz F. & Westfall P. 2013. Package “multcomp”. http://cran.stat.sfu.ca/web/packages/multcomp/multcomp.pdf (accessed 18.8.2013).Search in Google Scholar
Keddy P.A. 1992: Assembly and response rules: two goals for predictive community ecology. J. Veg. Sci. 3: 157–164.10.2307/3235676Search in Google Scholar
Keddy P.A., Wisheu I.C., Shippley B. & Gaudet C. 1989. Seed banks and vegetation management for conservation: toward predictive community ecology, pp. 347–365. In: Leck M.A., Parker V.T. & Simpson R.L. (eds), Ecology of Soil Seed Banks. Academic Press, San Diego.10.1016/B978-0-12-440405-2.50021-XSearch in Google Scholar
Kettenring K.M. & Galatowitsch S.M. 2007. Temperature requirements for dormancy break and seed germination vary greatly among 14 wetland Carex species. Aquat. Bot. 87: 209-220.10.1016/j.aquabot.2007.06.003Search in Google Scholar
Kettenring K.M. & Galatowitsch S.M. 2011a. Seed rain of restored and natural prairie wetlands. Wetlands 31: 283–294.10.1007/s13157-011-0159-6Search in Google Scholar
Kettenring K.M. & Galatowitsch S.M. 2011b. Carex seedling emergence in restored and natural prairie wetlands. Wetlands 31: 273-281.10.1007/s13157-011-0160-0Search in Google Scholar
Leck M.A. & Schütz W. 2005. Regeneration of Cyperaceae, with particular reference to seed ecology and seed banks. Perspect. Plant Ecol. Evol. Syst. 7: 95-133.10.1016/j.ppees.2005.05.001Search in Google Scholar
Mayfield M.M. & Levine J.M. 2010. Opposing effects of competitive exclusion on the phylogenetic structure of communities. Ecol. Lett. 13: 1085-1093.10.1111/j.1461-0248.2010.01509.xSearch in Google Scholar
McCullagh P. & Nelder J. A. 1989.Generalized Linear Models, 2nd edition. Chapman & Hall, London, 532 pp.10.1007/978-1-4899-3242-6Search in Google Scholar
Narbona E., Delgado A., Encina F., Miguez M. & Buide M.L. 2013. Seed germination and seedling establishment of the rare Carex helodes Link depend on the proximity to water. Aquat. Bot. 110: 55-60.10.1016/j.aquabot.2013.05.005Search in Google Scholar
Schütz W. 1997a. Are germination strategies important for the ability of cespitose wetland sedges (Carex) to grow in forests? Can. J. Bot. 75: 1692-1699.10.1139/b97-883Search in Google Scholar
Schütz W. 1997b. Primary dormancy and annual dormancy cycles in seeds of six temperate wetland sedges. Aquat. Bot. 59: 75— 85.10.1016/S0304-3770(97)00028-4Search in Google Scholar
Schütz W. 2000. Ecology of seed dormancy and germination in sedges (Carex). Perspect. Plant Ecol. Evol. Syst. 3: 67-89.10.1078/1433-8319-00005Search in Google Scholar
Schütz W. & Rave G. 1999. The effect of cold stratification and light on the seed germination of temperate sedges (Carex) from various habitats and implications for regenerative strategies. Plant Ecol. 144: 215-230.10.1023/A:1009892004730Search in Google Scholar
Schütz W. & Rave G. 2003. Variation in seed dormancy of the wetland sedge, Carex elongata, between populations and individuals in two consecutive years. Seed Sci. Res. 13: 315–322.10.1079/SSR2003148Search in Google Scholar
Silvertown J. 2004. Plant coexistence and the niche. Trends Ecol. Evol. 19: 605-611.10.1016/j.tree.2004.09.003Search in Google Scholar
Thompson K., Bakker J.P. & Bekker R.M. 1997. The soil seed banks of North West Europe: methodology, density and longevity. Cambridge university press, Cambridge, 288 pp.Search in Google Scholar
Tolasz R., Miková T. & Valeriánová A. 2007. Climate atlas of Czechia. ČHMÚ, Praha & UP, Olomouc.Search in Google Scholar
Van der Valk A.G., Bremholm T.L. & Gordon E. 1999. The restoration of sedge meadows: seed viability, seed germination requirements, and seedling growth of Carex species. Wetlands 19: 756-764.10.1007/BF03161782Search in Google Scholar
Van der Valk A.G. & Pederson R.L. 1989. Seed banks and the management and restoration of natural vegetation, pp. 329— 346. In: Leck M.A., Parker V.T. & Simpson R.L. (eds), Ecology of Soil Seed Banks, Academic Press, San Diego.10.1016/B978-0-12-440405-2.50020-8Search in Google Scholar
Van Kleunen M., Fischer M. & Schmid B. 2002. Experimental life-history evolution: selection on the allocation to sexual reproduction and its plasticity in a clonal plant. Evolution 56: 2168-2177.10.1111/j.0014-3820.2002.tb00141.xSearch in Google Scholar
Venables W.N. & Ripley B.D. 1998. Modern Applied Statistics with S-Plus. Springer-Verlag, New York, 447 pp.10.1007/978-1-4757-3121-7_14Search in Google Scholar
© 2017 Institute of Botany, Slovak Academy of Sciences
Articles in the same Issue
- Celluar and Molecular Biology
- Occurrence of Anaplasma phagocytophilum in three sympatric tick species in the South Moravia, Czech Republic
- Botany
- Germination strategies of two dominant Carex species in a swamp alder forest: implications for restoration
- Botany
- Role of ethylene and phospholipid-mediated signalling in mycotoxin-induced programmed cell death in the apical part of maize roots
- Botany
- Transcriptome analysis in leaves of rice (Oryza sativa) under high manganese stress
- Botany
- Type II metacaspase protein localization and gene transcription during programmed cell semi-death of sieve elements in developing caryopsis of Tritium aestivum
- Zoology
- Environmental factors determining the distribution pattern of leeches (Clitellata: Hirudinida) in large river systems: A case study of the Oder River system
- Zoology
- Effects of beaver dams on the zooplankton assemblages in four temperate lowland streams (NW Poland)
- Zoology
- Bioassessment of streams based on macroinvertebrates — can sampling of some substrate types be excluded?
- Zoology
- Communities of oribatid mites (Acari: Oribatida) of naturally regenerating and salvage-logged montane spruce forests of Šumava Mountains
- Zoology
- Food digestibility and consumption rate in detrito-bryophagous groundhopper Tetrix subulata (Orthoptera: Tetrigidae)
- Zoology
- Dietary alpha-ketoglutarate partially prevents age-related decline in locomotor activity and cold tolerance in Drosophila melanogaster
- Zoology
- The evaluation of endocrine regulators after intramuscular and oral application of cyanogenic glycoside amygdalin in rabbits
Articles in the same Issue
- Celluar and Molecular Biology
- Occurrence of Anaplasma phagocytophilum in three sympatric tick species in the South Moravia, Czech Republic
- Botany
- Germination strategies of two dominant Carex species in a swamp alder forest: implications for restoration
- Botany
- Role of ethylene and phospholipid-mediated signalling in mycotoxin-induced programmed cell death in the apical part of maize roots
- Botany
- Transcriptome analysis in leaves of rice (Oryza sativa) under high manganese stress
- Botany
- Type II metacaspase protein localization and gene transcription during programmed cell semi-death of sieve elements in developing caryopsis of Tritium aestivum
- Zoology
- Environmental factors determining the distribution pattern of leeches (Clitellata: Hirudinida) in large river systems: A case study of the Oder River system
- Zoology
- Effects of beaver dams on the zooplankton assemblages in four temperate lowland streams (NW Poland)
- Zoology
- Bioassessment of streams based on macroinvertebrates — can sampling of some substrate types be excluded?
- Zoology
- Communities of oribatid mites (Acari: Oribatida) of naturally regenerating and salvage-logged montane spruce forests of Šumava Mountains
- Zoology
- Food digestibility and consumption rate in detrito-bryophagous groundhopper Tetrix subulata (Orthoptera: Tetrigidae)
- Zoology
- Dietary alpha-ketoglutarate partially prevents age-related decline in locomotor activity and cold tolerance in Drosophila melanogaster
- Zoology
- The evaluation of endocrine regulators after intramuscular and oral application of cyanogenic glycoside amygdalin in rabbits