Startseite Lebenswissenschaften Molecular detection of Mycobacterium tuberculosis complex in the 8th century skeletal remains from the territory of Slovakia
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Molecular detection of Mycobacterium tuberculosis complex in the 8th century skeletal remains from the territory of Slovakia

  • Klaudia Kyselicová EMAIL logo , Lukáš Šebest EMAIL logo , Csaba Bognár , Michal Šarkan , Marián Baldovič , Radoslav Beňuš und Ľudevít Kádaši
Veröffentlicht/Copyright: 14. Juli 2016
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Biologia
Aus der Zeitschrift Biologia Band 71 Heft 6

Abstract

DNA was extracted using a Silica Bead Extraction kit from bone samples taken from a Slavonic-Avar individual found at the archaeological site of Cífer-Pác. The analysed skeletal remains from the grave number 62/79 belong to a young adult male (20–30 years at death) and are dated to the 8th–9th century anno Domini. The isolated ancient DNA (aDNA) was amplified by a targeted PCR with a primer pair designed to recognize the Mycobacterium tuberculosis complex insertion sequence IS6110. The aim of this molecular approach was to test and optimize a methodology for aDNA M. tuberculosis complex extraction from bone samples with osteological evidence of tuberculosis. Despite of the currently biased authenticity of the mentioned fragment, in this case study we prove that macroscopic evidence for tuberculosis additionally supported by a positive result of molecular testing can be considered authentic enough to be the proof of a tuberculosis infection caused by MTBC (Mycobacterium tuberculosis complex) bacteria when additional skeletal trauma and changes potentially caused by MOTT (mycobacteria other than tuberculosis) bacteria can be excluded. Positivity was confirmed in all of the three samples (thoracic vertebrae, lumbar vertebrae and right femur). Our results confirmed the diagnosis of tuberculosis of the spine and right hip joint. This is the first molecular evidence for the occurrence of tuberculosis on the territory of Slovakia. Through this bio-molecular approach we wish to provide a basis for aDNA examinations on other skeletal collections and provide epidemiological data concerning historical populations living on the territory of Slovakia.

Key words: PCR; infectious disease; insertion sequence; MTBC; Cífer-Pác; Slavonic-Avar population

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Abbreviations
AD

anno Domini

aDNA

ancient DNA

BC

before Christ

MOTT

mycobacteria other than tuberculosis

MTBC

Mycobacterium tuberculosis complex

NTM

non-tuberculous mycobacteria

TBC

tuberculosis.

Received: 2016-1-14
Accepted: 2016-6-3
Published Online: 2016-7-14
Published in Print: 2016-6-1

©2016 Institute of Molecular Biology, Slovak Academy of Sciences

Artikel in diesem Heft

  1. Cellular and Molecular Biology
  2. Molecular detection of Mycobacterium tuberculosis complex in the 8th century skeletal remains from the territory of Slovakia
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https://doi.org/10.1515/biolog-2016-0088
Schlagwörter für diesen Artikel
PCR; infectious disease; insertion sequence; MTBC; Cífer-Pác; Slavonic-Avar population

Artikel in diesem Heft

  1. Cellular and Molecular Biology
  2. Molecular detection of Mycobacterium tuberculosis complex in the 8th century skeletal remains from the territory of Slovakia
  3. Cellular and Molecular Biology
  4. First report of microorganisms of Caucasus glaciers (Georgia)
  5. Cellular and Molecular Biology
  6. Codon optimization of Aspergillus niger feruloyl esterase and its expression in Pichia pastoris
  7. Botany
  8. Response of lichens Cladonia arbuscula subsp. mitis and Cladonia furcata to nitrogen excess
  9. Botany
  10. No confirmation for previously suggested presence of diploid cytotypes of Sesleria (Poaceae) on the Balkan Peninsula
  11. Botany
  12. RCD1 homologues and their constituent WWE domain in plants: analysis of conservation through phylogeny methods
  13. Botany
  14. Nucleoli migration coupled with cytomixis
  15. Cellular and Molecular Biology
  16. Evaluation of appropriate reference gene for normalization of microRNA expression by real-time PCR in Lablab purpureus under abiotic stress conditions
  17. Zoology
  18. The fractal nature of the latitudinal biodiversity gradient
  19. Zoology
  20. A new species of Neoribates (Neoribates) (Acari: Oribatida: Parakalummidae) with key to the Neotropical species of the subgenus
  21. Zoology
  22. Diversity patterns of aquatic specialists and generalists: contrasts among two spring-fen mesohabitats and nearby streams
  23. Zoology
  24. Heteroptera (Insecta: Hemiptera) of the peat bogs of Belarusian Lakeland
  25. Cellular and Molecular Biology
  26. Cloning of monoacylglycerol o-acyltransferase 2 cDNA from a silkworm, Bombyx mori
  27. Zoology
  28. Biological aspect of the surface structure of the tongue in the adult red kangaroo (Macropus rufus) — light and scanning electron microscopy
  29. Zoology
  30. Status of the rose-ringed parakeet Psittacula krameri in Lisbon, Portugal
  31. Zoology
  32. Considerations on the vulnerability of the Eurasian water shrew Neomys fodiens to the presence of introduced brown trout Salmo trutta
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