Response of lichens Cladonia arbuscula subsp. mitis and Cladonia furcata to nitrogen excess
-
Ivana Králiková
,
Michal Goga
Abstract
In this study we focused on the comparison of the physiological responses to excess nitrogen (N) comparing Cladonia arbuscula subsp. mitis and Cladonia furcata. Both lichens were exposed to increased amounts of nitrogen (NH4NO3) for five weeks. Different concentrations of nitrogen were applied by spraying the lichen thalli during this period. After the treatment, the physiological parameters of the lichen, like chlorophyll a fluorescence, chlorophyll b integrity, content of soluble proteins, ergosterol, thiobarbituric acid reactive substances (TBARS) and content of secondary metabolites were measured. We found out that lichens responded differently to nitrogen excess concerning photobiont and mycobiont. The mycobiont of C. arbuscula subsp. mitis seems to be more sensitive to the nitrogen excess than C. furcata based on decreased ergosterol content. We concluded that the mycobiont is more sensitive to nitrogen excess than the photobiont in case of the tested lichen species in a middle term experiment.
Acknowledgements
This work was, in part, financially supported by Slovak Grant Agency (VEGA 1/0792/16). We thank Stephan Manhalter for critical reading and reviewing this manuscript.
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- Abbreviations
- HPLC
high performance liquid chromatography
- TBARS
thiobarbituric acid reactive species
- TCA
trichloracetic acid
- PhQ
phaeophytinization coefficient.
©2016 Institute of Botany, Slovak Academy of Sciences
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