Startseite Lebenswissenschaften Effect of supplemental feeds on liver and intestine of common carp (Cyprinus carpio) in semi-intensive rearing system: histological implications
Artikel
Lizenziert
Nicht lizenziert Erfordert eine Authentifizierung

Effect of supplemental feeds on liver and intestine of common carp (Cyprinus carpio) in semi-intensive rearing system: histological implications

  • Božidar Rašković EMAIL logo , Miloš Ćirić , Vesna Koko , Marko Stanković , Ivana živić , Zoran Marković und Vesna Poleksić
Veröffentlicht/Copyright: 25. März 2016
Veröffentlichen auch Sie bei De Gruyter Brill
Informationen für Autor*innen Erkunden Sie dieses Fachgebiet
Biologia
Aus der Zeitschrift Biologia Band 71 Heft 2

Abstract

In order to determine effects of different types of supplementary feed on the histology of liver and intestine of carp reared in a semi-intensive system, a six-month field experiment was carried out. Carp yearlings were fed different supplemental feeds: cereals (CF), pelleted (PF) and extruded (EF) compound feeds. Hepatocytes histomorphometry revealed larger nuclear profile area of EF-fed fish compared to other two groups, while cytoplasmic profile area of the hepatocytes decreased in following sequence: CF > EF > PF. Lower nucleus/cytoplasm ratio was found in CF-fed fish compared to EF-and PF-fed fish. Striking, season dependant finding, was that regardless of the type of supplementary feed the number of lipid droplets in hepatocytes was highest in July and August, lipid depletion occurred in September and October, while glycogen was present in hepatocytes throughout the study. No size difference between groups was observed for enterocytes height, absorptive surface height and number of goblet cells in tunica mucosa, but a strong relationship between length of intestinal folds and fish weight was found. Histological analysis revealed a difference among three types of supplemental feed used in this field study: extruded or steam-pelleted feed induced better nutritional status of common carp in comparison to cereals. At the same time, histomorphometry indicated some differences between EF-and PF-fed fish.

Key words: common carp; supplementary feeding; hepatocytes; enterocytes; histomorphometry

Acknowledgements

The study was supported by project of Ministry of Education and Science and Technological Development of the Republic of Serbia (TR31075) and FP7 project AREA (316004).

References

Baeverfjord G. & Krogdahl A. 1996. Development and regression of soybean meal induced enteritis in Atlantic salmon, Salmo salar L., distal intestine: a comparison with the intestines of fasted fish. J. Fish Dis. 19(5): 375–387. DOI: 10.1046/j.1365-2761.1996.d01-92.x10.1046/j.1365-2761.1996.d01-92.xSuche in Google Scholar

Boyd C.E. 1982. Water quality management of pond fish culture. Elsevier, Amsterdam, The Netherlands, 318 pp. ISBN-10: 0444420541, ISBN-13: 978-0444420541Suche in Google Scholar

Burel C., Boujard T., Tulli F. & Kaushik S.J. 2000. Digestibility of extruded peas, extruded lupin, and rapeseed meal in rainbow trout (Oncorhynchus mykiss)and turbot (Psetta maxima). Aquaculture 188 (3-4): 285–298. DOI: http://dx.doi.org/10.1016/S0044-8486(00)00337-910.1016/S0044-8486(00)00337-9Suche in Google Scholar

Caballero M.J., Izquierdo M.S., Kjorsvik E., Fernandez A.J. & Rosenlund G. 2004. Histological alterations in the liver of sea bream, Sparus aurata L., caused by short-or long-term feeding with vegetable oils. Recovery of normal morphology after feeding fish oil as the sole lipid source. J. Fish Dis. 27 (9): 531–541. DOI: 10.1111/j.1365-2761.2004.00572.x10.1111/j.1365-2761.2004.00572.xSuche in Google Scholar

Caballero M.J., López-Calero G., Socorro J., Roo F.J., Izquierdo M.S. & Férnandez A.J. 1999. Combined effect of lipid level and fish meal quality on liver histology of gilthead seabream (Sparus aurata). Aquaculture 179: 277–290.10.1016/S0044-8486(99)00165-9Suche in Google Scholar

Couch J.A. 1993. Light and electron microscopic comparisons of normal hepatocytes and neoplastic hepatocytes of well-differentiated hepatocellular carcinomas in a teleost fish. Dis. Aquat. Org. 16 (1): 1–14.10.3354/dao016001Suche in Google Scholar

Ćirić M., Subakov-Simić G., Dulić Z., BjelanovićK., Čičovački S. & Marković Z. 2015. Effect of supplemental feed type on water quality, plankton and benthos availability and carp (Cyprinus carpio L.) growth in semi-intensive monoculture ponds. Aquacult. Res. 46 (4): 777–788. DOI: 10.1111/are.1223010.1111/are.12230Suche in Google Scholar

Escaffre A., Kaushik S. & Mambrini M. 2007. Morphometric evaluation of changes in the digestive tract of rainbow trout (Oncorhynchus mykiss) due to fish meal replacement with soy protein concentrate. Aquaculture 273 (1): 127–138. DOI: http://dx.doi.org/10.1016/j.aquaculture.2007.09.02810.1016/j.aquaculture.2007.09.028Suche in Google Scholar

Fishelson L. & Becker K. 2001. Development and aging of the liver and pancreas in the domestic carp, Cyprinus carpio: from embryogenesis to 15-year-old fish. Environ. Biol. Fishes 61 (1): 85–97. DOI: 10.1023/A:101107672250510.1023/A:1011076722505Suche in Google Scholar

Foster G.D. & Moon T.W. 1991. Hypometabolism with fasting in the yellow perch (Perca flavescens): a study of enzymes, hepatocyte metabolism, and tissue size. Physiol. Zool. 64 (1): 259–275. DOI: 10.2307/3015852310.2307/30158523Suche in Google Scholar

Francis G., Makkar H.P.S. & Becker K. 2001. Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish. Aquaculture 199 (3-4): 197–227. DOI: 10.1016/S0044-8486(01)00526-910.1016/S0044-8486(01)00526-9Suche in Google Scholar

Hardy R.W. 1996. Alternate protein sources for salmon and trout diets. Anim. Feed Sci. Technol. 59 (1-3): 71–80. DOI: 10.1016/0377-8401(95)00888-810.1016/0377-8401(95)00888-8Suche in Google Scholar

Hlaváč D., Adámek Z., Hartman P. & Másílko J. 2014. Effects of supplementary feeding in carp ponds on discharge water quality: a review. Aquacult. Int. 22 (1): 299–320. DOI: 10.1007/s10499-013-9718-610.1007/s10499-013-9718-6Suche in Google Scholar

Horváth L., Tamás G. & Seagreave C. 2002. Carp and pond fish culture. 2nded. Blackwell Science, Oxford, England, 188 pp. ISBN: 978-0-85238-282-010.1002/9780470995662Suche in Google Scholar

Kamaszewski M., Napora-Rutkowski L. & Ostaszewska T. 2010. Effect of feeding on digestive enzyme activity and morphological changes in the liver and pancreas of pike-perch (Sander lucioperca). Israeli Journal of Aquaculture – Bamidgeh 62 (4): 225–236.Suche in Google Scholar

Krogdahl Å., Hemre G.I. & Mommsen T.P. 2005. Carbohydrates in fish nutrition: digestion and absorption in postlarval stages. Aquacult. Nutr. 11 (2): 103–122. DOI: 10.1111/j.1365-2095.2004.00327.x10.1111/j.1365-2095.2004.00327.xSuche in Google Scholar

Mandrioli L., Sirri R., Gatta P.P., Morandi F., Sarli G., Parma L., Fontanillas R. & Bonaldo A. 2012. Histomorphologic hepatic features and growth performances of juvenile Senegalese sole (Solea senegalensis) fed isogenertic practical diets with variable protein/lipid levels. J. Appl. Ichthyol. 28 (4): 628–632. DOI: 10.1111/j.1439-0426.2012.01938.x10.1111/j.1439-0426.2012.01938.xSuche in Google Scholar

Manera M., Visciano P., Losito P. & Ianieri A. 2003. Farmed fish pathology: quality aspects. Vet. Res. Commun. 27 (Suppl. 1): 695–698. DOI: 10.1023/B:VERC.0000014250.43833.1710.1023/B:VERC.0000014250.43833.17Suche in Google Scholar

Opstvedt J., Nygård E., Samuelsen T.A., Venturini G., Luzzana U. & Mundheim H. 2003. Effect on protein digestibility of different processing conditions in the production of fish meal and fish feed. J. Sci. Food Agric. 83 (8): 775–782. DOI: 10.1002/jsfa.139610.1002/jsfa.1396Suche in Google Scholar

Ostaszewska T., Dabrowski K., Hliwa P., Gomólka P. & Kwasek K. 2008. Nutritional regulation of intestine morphology in larval cyprinid fish, silver bream (Vimba vimba). Aquacult. Res. 39 (12): 1268–1278. DOI: 10.1111/j.1365-2109.2008.01989.x10.1111/j.1365-2109.2008.01989.xSuche in Google Scholar

Ostaszewska T., Dabrowski K., Palacios M.E., Olejniczak M. & Wieczorek M. 2005. Growth and morphological changes in the digestive tract of rainbow trout (Oncorhynchus mykiss) and pacu (Piaractus mesopotamicus) due to casein replacement with soybean proteins. Aquaculture 245 (1-4): 273–286. DOI: 10.1016/j.aquaculture.2004.12.00510.1016/j.aquaculture.2004.12.005Suche in Google Scholar

Poleksić V., Stanković M., Marković Z., RelićR., LakićN., DulićZ. & Rašković B. 2014. Morphological and physiological evaluation of common carp (Cyprinus carpio L., 1758) fed extruded compound feeds containing different fat levels. Aquacult. Int. 22 (1): 289–298. DOI: 10.1007/s10499-013-9654-510.1007/s10499-013-9654-5Suche in Google Scholar

Rasband W.S. 2011. ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA. http://rsb.info.nih.gov/ij/, 1997– 2011.Suche in Google Scholar

Rašković B., Čičovački S., CirićM., MarkovićZ. & PoleksićV. 2016. Integrative approach of histopathology and histomorphometry of common carp (Cyprinus carpio L.) organs as a marker of general fish health state in pond culture. Aquacult. Res. DOI: 10.1111/are.12795Suche in Google Scholar

RaškovićB., Jarić I., Koko V., Spasić M., DulićZ., Marković Z. & Poleksić V. 2013. Histopathological indicators: a useful fish health monitoring tool in common carp (Cyprinus carpio Linnaeus, 1758) culture. Cent. Eur. J. Biol. 8 (10):975–985. DOI: 10.2478/s11535-013-0220-y10.2478/s11535-013-0220-ySuche in Google Scholar

Raškovic B., Stanković M., DulićZ., MarkovićZ., LakićN. & Poleksić V. 2009. Effects of different source and level of protein in feed mixtures on liver and intestine histology of the common carp (Cyprinus carpio, Linnaeus, 1758). Comp. Bioch. Physiol. A Mol. Integr. Physiol. 153 (Suppl.2):S112. DOI: 10.1016/j.cbpa.2009.04.16310.1016/j.cbpa.2009.04.163Suche in Google Scholar

Rašković B.S., Stanković M.B., Marković Z.Z. & PoleksićV.D. 2011. Histological methods in the assessment of different feed effects on liver and intestine of fish. J. Agric. Sci. 56 (1): 87–100. DOI: 10.2298/JAS1101087R10.2298/JAS1101087RSuche in Google Scholar

Refstie S., Korsøen Ø.J., Storebakken T., Baeverfjord G., Lein I. & Roem A.J. 2000. Differing nutritional responses to dietary soybean meal in rainbow trout (Oncorhynchus mykiss)and Atlantic salmon (Salmo salar). Aquaculture 190 (1-2): 49–63. DOI: 10.1016/S0044-8486(00)00382-310.1016/S0044-8486(00)00382-3Suche in Google Scholar

Robaina L., Izquierdo M.S., Moyano F.J., Socorro J., Vergara J.M., Montero D. & Fernández-Palacios H. 1995. Soybean and lupin seed meals as protein sources in diets for gilthead seabream (Sparus aurata): nutritional and histological implications. Aquaculture 130 (2-3): 219–233. DOI: 10.1016/0044-8486(94)00225-D10.1016/0044-8486(94)00225-DSuche in Google Scholar

Saraiva A., Costa J., Serrăo J., Cruz C. & Eiras J.C. 2015. A histology-based fish health assessment of farmed seabass (Dicentrarchus labrax L.). Aquaculture 448: 375–381. DOI: 10. 1016/j.aquaculture.2015.02.00410. 1016/j.aquaculture.2015.02.004Suche in Google Scholar

Segner H. & Juario J.V. 1986. Histological observations on the rearing of milkfish, Chanos chanos, fry using different diets. J. Appl. Ichthyol. 2 (4): 162–172. DOI: 10.1111/j.1439-0426.1986.tb00658.x10.1111/j.1439-0426.1986.tb00658.xSuche in Google Scholar

Shepherd C.J. & Jackson A.J. 2013. Global fishmeal and fish-oil supply: inputs, outputs and markets. J. Fish Biol. 83 (4): 1046–1066. DOI: 10.1111/jfb.1222410.1111/jfb.12224Suche in Google Scholar

Shimeno S., Shikata T., Hosokawa H., Masumoto T. & Kheyyali D. 1997. Metabolic response to feeding rates in common carp, Cyprinus carpio.Aquaculture 151 (1): 371–377. DOI: http://dx.doi.org/10.1016/S0044-8486(96)01492-510.1016/S0044-8486(96)01492-5Suche in Google Scholar

Sørensen M. 2012. A review of the effects of ingredient composition and processing conditions on the physical qualities of extruded high-energy fish feed as measured by prevailing methods. Aquacult. Nutr. 18 (3): 233–248. DOI: 10.1111/j.1365-2095.2011.00924.x10.1111/j.1365-2095.2011.00924.xSuche in Google Scholar

Speare D.J. 2000. Liver diseases of tropical fish. Semin. Avian Exot. Pet. 9 (3): 174–178. DOI: http://dx.doi.org/10.1053/ax.2000.713210.1053/ax.2000.7132Suche in Google Scholar

Spisni E., Tugnoli M., Ponticelli A., Mordenti T. & Tomasi V. 1998. Hepatic steatosis in artificially fed marine teleosts. J. Fish Dis. 21 (3): 177–184. DOI: 10.1046/j.1365-2761.1998. 00089.x10.1046/j.1365-2761.1998. 00089.xSuche in Google Scholar

Stanković M.B., DulićZ.P. &Marković Z.Z. 2011. Protein sources and their significance in carp (Cyprinus carpio L.) nutrition. J. Agric. Sci. 56 (1): 75–86. DOI: 10.2298/JAS1101075S10.2298/JAS1101075SSuche in Google Scholar

Stone D.A.J. 2003. Dietary carbohydrate utilization by fish. Rev. Fish. Sci. 11 (4): 337–369. DOI: 10.1080/1064126039026088410.1080/10641260390260884Suche in Google Scholar

Strüssmann C.A. & Takashima F. 1990. Hepatocyte nuclear size and nutritional condition of larval pejerrey, Odontesthes bonariensis (Cuvier et Valenciennes). J. Fish Biol. 36 (1): 59–65. DOI: 10.1111/j.1095-8649.1990.tb03519.x10.1111/j.1095-8649.1990.tb03519.xSuche in Google Scholar

Svihus B., Uhlen A.K. & Harstad O.M. 2005. Effect of starch granule structure, associated components and processing on nutritive value of cereal starch: A review. Anim. Feed Sci. Technol. 122 (3-4): 303–320. DOI: http://dx.doi.org/10.1016/j.anifeedsci.2005.02.02510.1016/j.anifeedsci.2005.02.025Suche in Google Scholar

Urán P.A., Goncalves A.A., Taverne-Thiele J.J., Schrama J.W., Verreth J.A.J. & Rombout J.H.W.M. 2008. Soybean meal induces intestinal inflammation in common carp (Cyprinus carpio L.). Fish Shellfish Immunol. 25 (6): 751–760. DOI: 10.1016/j.fsi.2008.02.013.10.1016/j.fsi.2008.02.013Suche in Google Scholar PubMed

Weibel E.R., Kistler G.S. & Scherle W.F. 1966. Practical stereological methods for morphometric cytology. J. Cell Biol. 30 (1): 23–38. DOI: 10.1083/jcb.30.1.2310.1083/jcb.30.1.23Suche in Google Scholar PubMed PubMed Central

Zakęś Z., Kowalska A., Demska-Zakęś K., Jeney G. & Jeney Z. 2008. Effect of two medicinal herbs (Astragalus radix and Lonicera japonica) on the growth performance and body composition of juvenile pikeperch [Sander lucioperca (L.)]. Aquacult. Res. 39 (11): 1149–1160. DOI: 10.1111/j.1365-2109.2008.01977.x10.1111/j.1365-2109.2008.01977.xSuche in Google Scholar

Zheng Q., Wu Y. & Xu H. 2015. Effect of dietary oxidized konjac glucomannan on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora. Fish Physiol. Biochem. 41 (3): 733–743. DOI: 10.1007/s10695-015-0042-0.10.1007/s10695-015-0042-0Suche in Google Scholar PubMed

Received: 2015-8-21
Accepted: 2015-12-16
Published Online: 2016-3-25
Published in Print: 2016-2-1

© 2016 Institute of Zoology, Slovak Academy of Sciences

Artikel in diesem Heft

  1. Cellular and Molecular Biology
  2. Antimicrobial resistance and molecular characterisation of human campylobacters from Slovakia
  3. Cellular and Molecular Biology
  4. Application of violet pigment from Chromobacterium violaceum UTM5 in textile dyeing
  5. Cellular and Molecular Biology
  6. Chemical composition and antioxidant activity of some important betel vine landraces
  7. Cellular and Molecular Biology
  8. Development of conventional and real time PCR assay for detection and quantification of Rhizoctonia solani infecting pulse crops
  9. Botany
  10. Somatic embryogenesis and in vitro shoot propagation of Gentianautriculosa
  11. Botany
  12. Effect of cold treatment on germination of Saxifraga aizoides and S. paniculata: a comparison of central (eastern Alps) and southern populations (northern Apennines)
  13. Botany
  14. Environmental factors and phytohormones enhancing expression of α-momorcharin gene in Momordica charantia
  15. Botany
  16. Unveiling the kinematics of the avoidance response in maize (Zen mays) primary roots
  17. Zoology
  18. Review of the species allocated to the genus Satchellius (Oligochaeta: Lumbricidae) with description of a new species
  19. Cellular and Molecular Biology
  20. Cation metals specific hemocyanin exhibits differential antibacterial property in mud crab, Scylla serrata
  21. Zoology
  22. Dynamics of soil Collembola communities (Hexapoda: Collembola) along the mesoclimatic gradient in a deep karst valley
  23. Zoology
  24. Hidden invertebrate diversity – phytotelmata in Bromeliaceae from palm houses and florist wholesalers (Poland)
  25. Zoology
  26. Species composition of mosquitoes (Diptera: Culicidae) in relation to climate conditions in South-Eastern Slovakia
  27. Zoology
  28. Effect of supplemental feeds on liver and intestine of common carp (Cyprinus carpio) in semi-intensive rearing system: histological implications
  29. Zoology
  30. Trace element enrichment in the eggshells of Grus japonensis and its association witheggshell thinningin ZhalongWetland (Northeastern China)
https://doi.org/10.1515/biolog-2016-0017
Schlagwörter für diesen Artikel
common carp; supplementary feeding; hepatocytes; enterocytes; histomorphometry

Artikel in diesem Heft

  1. Cellular and Molecular Biology
  2. Antimicrobial resistance and molecular characterisation of human campylobacters from Slovakia
  3. Cellular and Molecular Biology
  4. Application of violet pigment from Chromobacterium violaceum UTM5 in textile dyeing
  5. Cellular and Molecular Biology
  6. Chemical composition and antioxidant activity of some important betel vine landraces
  7. Cellular and Molecular Biology
  8. Development of conventional and real time PCR assay for detection and quantification of Rhizoctonia solani infecting pulse crops
  9. Botany
  10. Somatic embryogenesis and in vitro shoot propagation of Gentianautriculosa
  11. Botany
  12. Effect of cold treatment on germination of Saxifraga aizoides and S. paniculata: a comparison of central (eastern Alps) and southern populations (northern Apennines)
  13. Botany
  14. Environmental factors and phytohormones enhancing expression of α-momorcharin gene in Momordica charantia
  15. Botany
  16. Unveiling the kinematics of the avoidance response in maize (Zen mays) primary roots
  17. Zoology
  18. Review of the species allocated to the genus Satchellius (Oligochaeta: Lumbricidae) with description of a new species
  19. Cellular and Molecular Biology
  20. Cation metals specific hemocyanin exhibits differential antibacterial property in mud crab, Scylla serrata
  21. Zoology
  22. Dynamics of soil Collembola communities (Hexapoda: Collembola) along the mesoclimatic gradient in a deep karst valley
  23. Zoology
  24. Hidden invertebrate diversity – phytotelmata in Bromeliaceae from palm houses and florist wholesalers (Poland)
  25. Zoology
  26. Species composition of mosquitoes (Diptera: Culicidae) in relation to climate conditions in South-Eastern Slovakia
  27. Zoology
  28. Effect of supplemental feeds on liver and intestine of common carp (Cyprinus carpio) in semi-intensive rearing system: histological implications
  29. Zoology
  30. Trace element enrichment in the eggshells of Grus japonensis and its association witheggshell thinningin ZhalongWetland (Northeastern China)
Jetzt anmelden und 20% sparen
Institutioneller Zugang
Wie funktioniert Authentifizierung?
Haben Sie eine Idee, wie wir unsere Website verbessern können?
Bitte schreiben Sie uns.
© 2025 De Gruyter Brill
Heruntergeladen am 8.12.2025 von https://www.degruyterbrill.com/document/doi/10.1515/biolog-2016-0017/pdf?lang=de
Button zum nach oben scrollen