Antimicrobial resistance and molecular characterisation of human campylobacters from Slovakia
-
Juraj Hanzen
Abstract
This paper delivers molecular biological study of antimicrobial resistance in clinical isolates of Campylobacter spp. in Slovakia. During the year 2015 a total of 828 Campylobacter strains collected from five regions in Slovakia were examined for resistance to ciprofloxacin (CIP), tetracycline (TET), erythromycin (ERY) and ampicillin with sulbactam (SAM). The highest incidence of Campylobacter resistance was observed for CIP (64.8%), followed by TET (30.5%) and SAM (26.3%). All strains were susceptible to ERY. Twenty selected antibiotic-resistant strains were examined for the presence Campylobacter β-lactamase OXA-61, multidrug efflux pump cmeB and TET tet(O) genes by PCR. From twenty CIP-resistant strains, the cmeB gene was present in 12, while the chromosomal OXA-61 β-lactamase occurred in 11 strains from 18 SAM-resistant Campylobacters, and tet(O) was present in 14 strains from 18 TET-resistant ones investigated. All three genes together occurred within a single strain in five Campylobacter jejuni and one Campylobacter coli strains isolated from eastern Slovakia. Clonal relatedness of strains was detected by a MALDI biotyper, which revealed two clusters of C. jejuni and one cluster of C. coli. The results showed that the epidemiologically important strains with high fluoroquinolone resistance occurred among the Campylobacter strains. An important finding is represented also by the evidence of the OXA-61 β-lactamase in more than one half of investigated strains, because SAM is frequently used for therapy in children and pregnant women.
Acknowledgements
We are grateful to all of the microbiologists and technicians of the HPL spol. s r.o. that collected the isolates for us. This work was supported by funding from the Slovak Research and Development Agency (grant No. APVV-14-0274) and from ERDF No. 26220220065.
References
Abd El-Baky R.M., Sakhy M. & Gad G. 2014. Antibiotic susceptibility pattern and genotyping of Campylobacter species isolated from children suffering from gastroenteritis. Indian J. Med. Microbiol. 32: 240–246.10.4103/0255-0857.136550Search in Google Scholar PubMed
Alfredson D.A. & Korolik V. 2005. Isolation and expression of a novel molecular class D, β-lactamase, OXA-61, from Campylobacter jejuni. Antimicrob. Agents Chemother. 49: 2515–2518.10.1128/AAC.49.6.2515-2518.2005Search in Google Scholar PubMed PubMed Central
Allos B.M. 2001. Campylobacter jejuni infections: update on emerging issues and trends. Clin. Infect. Dis. 32: 1201–1206.10.1086/319760Search in Google Scholar PubMed
Bardoň J., Kolář M., Karpisková R. & Hricová K. 2011. Prevalence of thermotolerant Campylobacter spp. in broilers at retail in the Czech Republic and their antibiotic resistance. Food Control 22: 328–332.10.1016/j.foodcont.2010.08.001Search in Google Scholar
Brtková A., Čuvalová Z. & Kantíková M. 2010. Analyses of the baseline survey on the prevalence on the Campylobacter spp. and Salmonella spp. in broilers in Slovakia. Proceedings of the Food Micro 2010, 30 August 3 September, Copenhagen, Denmark, Abstract No. PEA1.78, p. 108.Search in Google Scholar
CA SFM 2014. Comité de l’antibiogramme de la Société Française de Microbiologie – Recommandations 2014. http://www.sfm-microbiologie.org/UserFiles/files/casfm/CASFM-EUCAST-V1-0_2014(1).pdf (accessed 20.10.2015).Search in Google Scholar
Drugdová Z. & Kmeť V. 2013. Prevalence of β-lactam and fluoroquinolone resistance, and virulence factors in Escherichia coli isolated from chickens in Slovakia. Biologia 68: 11–17.10.2478/s11756-012-0142-6Search in Google Scholar
EUCAST 2015. The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 5.0, 2015. http://www.eucast.org (accessed 20.10.2015).Search in Google Scholar
ECDC, EFSA, EMEA & SCENIHR 2009. Joint Opinion on antimicrobial resistance (AMR) focused on zoonotic infections. EFSA J. 7: 1372.10.2903/j.efsa.2009.1372Search in Google Scholar
EFSA & ECDC. 2014. The European Union Summary Report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2012. EFSA J. 12: 3547.Search in Google Scholar
Ge B., McDermott P.F., White D.G. & Meng J. 2005. Role of efflux pumps and topoisomerase mutations in fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli. Antimicrob. Agents Chemother. 49: 3347–3354.10.1128/AAC.49.8.3347-3354.2005Search in Google Scholar PubMed PubMed Central
Griggs D.J., Peake L., Johnson M.M., Ghori S., Mott A. & Piddock L.J. 2009. β-Lactamase-mediated β-lactam resistance in Campylobacter species: prevalence of Cj0299 (bla OXA-61) and evidence for a novel β-lactamase in C. jejuni. Antimicrob. Agents Chemother. 53: 3357–3364.10.1128/AAC.01655-08Search in Google Scholar PubMed PubMed Central
Iovine N.M. 2013. Resistance mechanisms in Campylobacter jejuni. Virulence 4: 230–240.10.4161/viru.23753Search in Google Scholar PubMed PubMed Central
Kmet V., Drugdová Z., Kmetova M. & Stanko M. 2013. Virulence and antibiotic resistance of Escherichia coli isolated from rooks. Ann. Agric. Environ. Med. 20: 273–275.Search in Google Scholar
Moore J.E., Barton M.D., Blair I.S., Corcoran D., Dooley J.S., Fanning S., Kempf I., Lastovica A.J., Lowery C.J., Matsuda M., McDowell D.A., McMahon A., Millar B.C., Rao J.R., Rooney P.J., Seal B.S., Snelling W.J. & Tolba O. 2006. The epidemiology of antibiotic resistance in Campylobacter. Microb. Infect. 8: 1955–1966.10.1016/j.micinf.2005.12.030Search in Google Scholar PubMed
Ng L.K., Martin I., Alfa M. & Mulvey M. 2001. Multiplex PCR for the detection of tetracycline resistant genes. Mol. Cell. Probes 15: 209–215.10.1006/mcpr.2001.0363Search in Google Scholar PubMed
Obeng A.S., Rickard H., Sexton M., Pang Y., Peng H. & Barton M. 2012. Antimicrobial susceptibilities and resistance genes in Campylobacter strains isolated from poultry and pigs in Australia. J. Appl. Microbiol. 113: 294–307.10.1111/j.1365-2672.2012.05354.xSearch in Google Scholar PubMed
Olah P.A., Doetkott C., Fakhr M.K. & Logue C.M. 2006. Prevalence of the Campylobacter multi-drug efflux pump (Cme-ABC) in Campylobacter spp. isolated from freshly processed turkeys. Food Microbiol. 23: 453–460.10.1016/j.fm.2005.06.004Search in Google Scholar PubMed
Padungton P. & Kaneene J.B. 2003. Campylobacter spp. in human, chickens, pigs and their antimicrobial resistance. J. Vet. Med. Sci. 65: 161–170.10.1292/jvms.65.161Search in Google Scholar PubMed
von Altrock A., Hamedy A., Merle R. & Waldmann K.H. 2013. Campylobacter spp. – prevalence on pig livers and antimicrobial susceptibility. Prev. Veter. Med. 109: 152–157.10.1016/j.prevetmed.2012.09.010Search in Google Scholar PubMed
Wieczorek K., Szewczyk R. & Osek J. 2012. Prevalence, antimicrobial resistance, and molecular characterization of Campy-lobacter jejuni and C. coli isolated from retail raw meat in Poland. Vet. Med. 57: 293–299.10.17221/6016-VETMEDSearch in Google Scholar
Zeng X., Brown S., Gillespie B. & Lin J. 2014. A single nucleotide in the promoter region modulates the expression of the β-lactamase OXA-61 in Campylobacter jejuni. J. Antimicrob. Chemother. 69: 1215–1223.10.1093/jac/dkt515Search in Google Scholar PubMed
- Abbreviations
- CIP
ciprofloxacin
- ERY
erythromycin
- SAM
ampicillin with sulbactam
- TET
tetracycline
© 2016 Institute of Molecular Biology, Slovak Academy of Sciences
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- Cellular and Molecular Biology
- Antimicrobial resistance and molecular characterisation of human campylobacters from Slovakia
- Cellular and Molecular Biology
- Application of violet pigment from Chromobacterium violaceum UTM5 in textile dyeing
- Cellular and Molecular Biology
- Chemical composition and antioxidant activity of some important betel vine landraces
- Cellular and Molecular Biology
- Development of conventional and real time PCR assay for detection and quantification of Rhizoctonia solani infecting pulse crops
- Botany
- Somatic embryogenesis and in vitro shoot propagation of Gentianautriculosa
- Botany
- Effect of cold treatment on germination of Saxifraga aizoides and S. paniculata: a comparison of central (eastern Alps) and southern populations (northern Apennines)
- Botany
- Environmental factors and phytohormones enhancing expression of α-momorcharin gene in Momordica charantia
- Botany
- Unveiling the kinematics of the avoidance response in maize (Zen mays) primary roots
- Zoology
- Review of the species allocated to the genus Satchellius (Oligochaeta: Lumbricidae) with description of a new species
- Cellular and Molecular Biology
- Cation metals specific hemocyanin exhibits differential antibacterial property in mud crab, Scylla serrata
- Zoology
- Dynamics of soil Collembola communities (Hexapoda: Collembola) along the mesoclimatic gradient in a deep karst valley
- Zoology
- Hidden invertebrate diversity – phytotelmata in Bromeliaceae from palm houses and florist wholesalers (Poland)
- Zoology
- Species composition of mosquitoes (Diptera: Culicidae) in relation to climate conditions in South-Eastern Slovakia
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- Effect of supplemental feeds on liver and intestine of common carp (Cyprinus carpio) in semi-intensive rearing system: histological implications
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