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Multilayer-based lab-on-a-chip systems for perfused cell-based assays

  • Udo Klotzbach

    Udo Klotzbach obtained his degree in Electrical Engineering at the Technical University Dresden in 1995. Since July 1997 he has been employed at the Fraunhofer IWS Dresden. In 2006, he received a Dr. techn. degree in Mechanical Engineering and Science of Management from the University of Vienna. He is experienced in laser micro materials processing and currently leading the Micro Processing department. Dr. Udo Klotzbach is a project manager of the European IP project LIFT and ALABO.

     EMAIL logo     , Frank Sonntag

    Frank Sonntag received his diploma in Electrical Engineering in 2000 from the Technical University Dresden. Since 2003 he has been with the Fraunhofer Institute for Material and Beam Technology (IWS) Dresden. In 2010, he received a Dr.-Ing. degree in Mechanical Engineering from Dresden University. His main research interests are lab-on-a-chip-systems with integrated micropumps and biosensors based on fluorescence, surface plasmon, or electrical sensing. Currently he is leading the group Micro-/Biosystems in Fraunhofer IWS.

         , Stefan Grünzner

    Stefan Grünzner is writing his diploma thesis at the Fraunhofer Institute for Material and Beam Technology (IWS) Dresden. His main research interests technologies for microstructuring and microjoining of lab-on-a-chip-systems with integrated actuators.

         , Mathias Busek

    Mathias Busek studied Electrical Engineering in the field of Biomedical Engineering at Dresden University of Technology and gained his diploma degree in 2009. Afterwards he worked as a scientist at TU-Berlin for 3 years. Since 2013 he is a research fellow at Fraunhofer IWS in Dresden. For his PhD thesis he is working on microfluidic circuits and oxygen supply for cell culture system. The work is funded by the Federal Ministry of Economics and Technology within the project “SimFluNet” in cooperation with the ITI GmbH in Dresden.

         , Florian Schmieder

    Florian Schmieder received his diploma in Bioprocess Engineering in 2013 from the Technical University Dresden. Since 2013 he is employed at the Fraunhofer Institute for Material and Beam Technology (IWS) Dresden. His main topics in research are lab-on-a-chipsystems with integrated actuators and their applications in complex 3D-cell culture and artificial organ rebuilding.

         and Volker Franke

    Volker Franke received his diploma in Physical Technologies in 2003 from the University of Applied Sciences Mittweida. Since 2003 he has been with the Fraunhofer Institute for Material and Beam Technology (IWS) Dresden. His main research interests are laser micro material processing with short and ultra-short pulsed lasers.
Published/Copyright: December 18, 2014
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Advanced Optical Technologies
From the journal Advanced Optical Technologies Volume 3 Issue 5-6

Abstract

A novel integrated technology chain of laser-microstructured multilayer foils for fast, flexible, and low-cost manufacturing of lab-on-a-chip devices especially for complex cell and tissue culture applications, which provides pulsatile fluid flow within physiological ranges at low media-to-cells ratio, was developed and established. Initially the microfluidic system is constructively divided into individual layers, which are formed by separate foils or plates. Based on the functional boundary conditions and the necessary properties of each layer, their corresponding foils and plates are chosen. In the third step, the foils and plates are laser microstructured and functionalized from both sides. In the fourth and last manufacturing step, the multiple plates and foils are joined using different bonding techniques like adhesive bonding, welding, etc. This multilayer technology together with pneumatically driven micropumps and valves permits the manufacturing of fluidic structures and perfusion systems, which spread out above multiple planes. Based on the established lab-on-a-chip platform for perfused cell-based assays, a multilayer microfluidic system with two parallel connected cell culture chambers was successfully implemented.

Keywords: cell culture; lab-on-a-chip; laser microstructuring; micropump; microsystems; perfusion
Corresponding author: Udo Klotzbach, Fraunhofer Institute for Material and Beam Technology IWS, Winterbergstraße 28, 01277 Dresden, Germany, e-mail:

About the authors

Udo Klotzbach

Udo Klotzbach obtained his degree in Electrical Engineering at the Technical University Dresden in 1995. Since July 1997 he has been employed at the Fraunhofer IWS Dresden. In 2006, he received a Dr. techn. degree in Mechanical Engineering and Science of Management from the University of Vienna. He is experienced in laser micro materials processing and currently leading the Micro Processing department. Dr. Udo Klotzbach is a project manager of the European IP project LIFT and ALABO.

Frank Sonntag

Frank Sonntag received his diploma in Electrical Engineering in 2000 from the Technical University Dresden. Since 2003 he has been with the Fraunhofer Institute for Material and Beam Technology (IWS) Dresden. In 2010, he received a Dr.-Ing. degree in Mechanical Engineering from Dresden University. His main research interests are lab-on-a-chip-systems with integrated micropumps and biosensors based on fluorescence, surface plasmon, or electrical sensing. Currently he is leading the group Micro-/Biosystems in Fraunhofer IWS.

Stefan Grünzner

Stefan Grünzner is writing his diploma thesis at the Fraunhofer Institute for Material and Beam Technology (IWS) Dresden. His main research interests technologies for microstructuring and microjoining of lab-on-a-chip-systems with integrated actuators.

Mathias Busek

Mathias Busek studied Electrical Engineering in the field of Biomedical Engineering at Dresden University of Technology and gained his diploma degree in 2009. Afterwards he worked as a scientist at TU-Berlin for 3 years. Since 2013 he is a research fellow at Fraunhofer IWS in Dresden. For his PhD thesis he is working on microfluidic circuits and oxygen supply for cell culture system. The work is funded by the Federal Ministry of Economics and Technology within the project “SimFluNet” in cooperation with the ITI GmbH in Dresden.

Florian Schmieder

Florian Schmieder received his diploma in Bioprocess Engineering in 2013 from the Technical University Dresden. Since 2013 he is employed at the Fraunhofer Institute for Material and Beam Technology (IWS) Dresden. His main topics in research are lab-on-a-chipsystems with integrated actuators and their applications in complex 3D-cell culture and artificial organ rebuilding.

Volker Franke

Volker Franke received his diploma in Physical Technologies in 2003 from the University of Applied Sciences Mittweida. Since 2003 he has been with the Fraunhofer Institute for Material and Beam Technology (IWS) Dresden. His main research interests are laser micro material processing with short and ultra-short pulsed lasers.

Acknowledgments

The authors want to express great appreciation to the Free State of Saxony and the European Union (SAB project UNILOC) for the finance support.

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Received: 2014-9-7
Accepted: 2014-10-31
Published Online: 2014-12-18
Published in Print: 2014-12-1

©2014 THOSS Media & De Gruyter

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https://doi.org/10.1515/aot-2014-0046
Keywords for this article
cell culture; lab-on-a-chip; laser microstructuring; micropump; microsystems; perfusion

Articles in the same Issue

  1. Cover and Frontmatter
  2. Views
  3. III-V Multi-junction solar cells and concentrating photovoltaic (CPV) systems
  4. Lasers in Green Production: The best is yet to come
  5. From LED to laser fusion: Green Photonics in a US perspective
  6. Community
  7. News from the European Optical Society
  8. Conference Notes
  9. Conference Calendar
  10. Topical Issue: Green Photonics
  11. Editorial
  12. Green photonics: sustainable solutions for a future with light
  13. Review Articles
  14. Interferometric femtosecond laser processing for nanostructuring inside thin film
  15. Multilayer-based lab-on-a-chip systems for perfused cell-based assays
  16. System efficiency of laser-based white light
  17. Low-power consumption seamless wireless and wired links using transparent waveform transfer
  18. Research Articles
  19. Femtosecond laser full and partial texturing of steel surfaces to reduce friction in lubricated contact
  20. Multi-pass absorption spectroscopy for H2O2 detection using a CW DFB-QCL
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