Stimulation of hCG protein and mRNA in first trimester villous cytotrophoblast cells in vitro by glycodelin A
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Udo Jeschke
, Uwe Karsten , Toralf Reimer , Dagmar-Ulrike Richter , Claudia Bergemann , Volker Briese , Ioannis Mylonas and Klaus Friese
Abstract
Aim: Human chorionic gonadotropin (hCG) is produced by fetal trophoblast cells and secreted into maternal circulation mainly in the first trimester of pregnancy. Another glycoprotein, glycodelin A, is one of the main products of the maternal decidua during this period. The purpose of this study was to investigate the effect of glycodelin A on hCG release by isolated cytotrophoblast cells in vitro.
Methods: Cytotrophoblast cells were prepared from human first trimester placenta and incubated with varying concentrations of glycodelin A. Supernatants were assayed for hCG protein concentrations, and quantification of beta hCG mRNA was carried out by RT-PCR. Expression of hCG was analysed in stimulated trophoblast cells and in unstimulated controls by immunocytochemistry.
Results: Glycodelin A induces a dose-dependent increase of hCG production. An increase of hCG expression was measured at 100 and 200 μg/mL glycodelin-A treatment in trophoblast cell culture by TaqMan assay on mRNA level. We found a moderate staining of hCG in control trophoblast cells, whereas a strong hCG staining was seen in glycodelin A-treated trophoblast cells.
Conclusions: HCG is a marker for the differentiation process of trophoblast cells. Our results suggest that glycodelin A secreted by the decidualized endometrium is involved in the regulation of hormones produced by the trophoblast.
References
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Articles in the same Issue
- Pregnancy outcome of euploid fetuses with increased nuchal translucency: how bad is the news?
- Cerebral edema on MRI in severe preeclamptic women developing eclampsia
- Smoking during pregnancy and bulimia nervosa in offspring
- Stimulation of hCG protein and mRNA in first trimester villous cytotrophoblast cells in vitro by glycodelin A
- Umbilical artery Doppler and umbilical cord pH at birth in small-for-gestational-age fetuses: valid estimate of their relationship
- Hemodynamics of the renal artery and descending aorta in fetuses with renal disease using color Doppler ultrasound – longitudinal comparison to normal fetuses
- Echocardiographic measurements in infants of diabetic mothers and macrosomic infants of nondiabetic mothers
- Obstetric and neonatal outcomes in apparently isolated mild fetal ventriculomegaly
- Visual analysis of antepartum fetal heart rate tracings: inter- and intra-observer agreement and impact of knowledge of neonatal outcome
- Multiple positive results during a neonatal screening program: a retrospective analysis of incidence, clinical implications and outcomes
- Thrombocytopoiesis in healthy term newborns
- Infected neonatal cephalohematomas caused by anaerobic bacteria
- Endothelin-1 concentrations in cord blood of neonates with meconium-stained amniotic fluid
- Fetal alcohol syndrome and pyloric stenosis: alcohol induced or an association?
- Course of pregnancy and birth in a patient with Bernard-Soulier syndrome – a case report
Articles in the same Issue
- Pregnancy outcome of euploid fetuses with increased nuchal translucency: how bad is the news?
- Cerebral edema on MRI in severe preeclamptic women developing eclampsia
- Smoking during pregnancy and bulimia nervosa in offspring
- Stimulation of hCG protein and mRNA in first trimester villous cytotrophoblast cells in vitro by glycodelin A
- Umbilical artery Doppler and umbilical cord pH at birth in small-for-gestational-age fetuses: valid estimate of their relationship
- Hemodynamics of the renal artery and descending aorta in fetuses with renal disease using color Doppler ultrasound – longitudinal comparison to normal fetuses
- Echocardiographic measurements in infants of diabetic mothers and macrosomic infants of nondiabetic mothers
- Obstetric and neonatal outcomes in apparently isolated mild fetal ventriculomegaly
- Visual analysis of antepartum fetal heart rate tracings: inter- and intra-observer agreement and impact of knowledge of neonatal outcome
- Multiple positive results during a neonatal screening program: a retrospective analysis of incidence, clinical implications and outcomes
- Thrombocytopoiesis in healthy term newborns
- Infected neonatal cephalohematomas caused by anaerobic bacteria
- Endothelin-1 concentrations in cord blood of neonates with meconium-stained amniotic fluid
- Fetal alcohol syndrome and pyloric stenosis: alcohol induced or an association?
- Course of pregnancy and birth in a patient with Bernard-Soulier syndrome – a case report