IL-8 concentrations in maternal serum, amniotic fluid and cord blood in relation to different pathogens within the amniotic cavity
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Armin Witt
, Angelika Berger , Christian J. Gruber , Ljubomir Petricevic , Petra Apfalter and Peter Husslein
Abstract
Objective: The association between elevated interleukin (IL)-8 concentrations in amniotic fluid and preterm delivery is well described. Little consideration has been given to the impact of different groups of microorganisms within the amniotic cavity on IL-8 concentration.
Methods: We collected amniotic fluid, placental tissue and amniotic membranes during preterm cesarean sections for bacterial culture. In addition, we determined IL-8 concentrations in maternal serum, amniotic fluid and cord blood and correlated them with the various intra-amniotic pathogens isolated by bacterial culture.
Results: IL-8 concentrations were determined in amniotic fluid in 107 cases, in cord blood in 185 cases and in maternal blood in 158 cases. Women with intra-amniotic Ureaplasma urealyticum infection had significantly higher amniotic fluid concentrations of IL-8 than those without (P<0.001). In cord blood, we found significantly elevated IL-8 concentrations due to intra-amniotic infection with U. urealyticum (P=0.045) and other pathogens (P=0.04). In maternal sera, we found no significant elevation of maternal IL-8 in any of the groups.
Conclusion: Intrauterine infection with U. urealyticum seems to play a profound role in the cascade of inflammation and increases IL-8 concentrations in amniotic fluid and cord blood.
References
1 Abzug MJ, DW Hershey, HA Rotbart, MJ Levin: Incidence of nonbacterial intraamniotic infections in abnormal pregnancies. J Reprod Med36 (1991) 783Search in Google Scholar
2 Gauthier DW, WJ Meyer, A Bieniarz: Expectant management of premature rupture of membranes with amniotic fluid cultures positive for Ureaplasma urealyticum alone. Am J Obstet Gynecol170 (1994) 587Search in Google Scholar
3 Ghezzi F, R Gomez, R Romero: Elevated interleukin-8 concentrations in amniotic fluid of mothers whose neonates subsequently develop bronchopulmonary dysplasia. Eur J Obstet Gynecol Reprod Biol78 (1998) 5Search in Google Scholar
4 Hannaford K, DA Todd, H Jeffery, E John, K Blyth, GL Gilbert: Role of Ureaplasma urealyticum in lung disease of prematurity. Arch Dis Child Fetal Neonatal Ed81 (1999) 162Search in Google Scholar
5 Hsu CD, E Meaddough, K Aversa: Elevated amniotic fluid levels of leukemia inhibitory factor, interleukin 6, and interleukin 8 in intra-amniotic infection. Am J Obstet Gynecol179 (1998) 1267Search in Google Scholar
6 Jacobsson B, I Mattsby-Baltzer, B Andersch, H Bokstrom, RM Holst, UB Wennerholm, H Hagberg: Microbial invasion and cytokine response in amniotic fluid in a Swedish population of women in preterm labor. Acta Obstet Gynecol Scand82 (2003) 120Search in Google Scholar
7 Li YH, A Brauner, B Jonsson, I van der Ploeg, O Soder, M Holst, JS Jensen, H Lagercrantz, K Tullus: Ureaplasma urealyticum-induced production of proinflammatory cytokines by macrophages. Pediatr Res48 (2000) 114Search in Google Scholar
8 Ollikainen J, H Hiekkaniemi, M Korppi, H Sarkkinen, K Heinonen: Ureaplasma urealyticum infection associated with acute respiratory insufficiency and death in premature infants. J Pediatr122 (1993) 756Search in Google Scholar
9 Romero R, M Mazor, R Morrotti, C Avila, E Oyarzun, A Insunza, M Parra, E Behnke, F Montiel, GH Cassell: Infection and labor. VII. Microbial invasion of the amniotic cavity in spontaneous rupture of membranes at term. Am J Obstet Gynecol166 (1992) 129Search in Google Scholar
10 Shimoya K, N Matsuzaki, T Taniguchi, T Jo, F Saji, H Kitajima, M Fujimura, M Nakayama, O Tanizawa: Interleukin-8 in cord sera: a sensitive and specific marker for the detection of preterm chorioamnionitis. J Infect Dis165 (1992) 957Search in Google Scholar
11 von Minckwitz G, EM Grischke, S Schwab, S Hettinger, S Loibl, M Aulmann, M Kaufmann: Predictive value of serum interleukin-6 and -8 levels in preterm labor or rupture of the membranes. Acta Obstet Gynecol Scand79 (2000) 667Search in Google Scholar
12 Watts DH, MA Krohn, SL Hillier, DA Eschenbach: The association of occult amniotic fluid infection with gestational age and neonatal outcome among women in preterm labor. Obstet Gynecol79 (1992) 351Search in Google Scholar
13 Yoon BH, R Romero, JS Park, JW Chang, YA Kim, JC Kim, KS Kim: Microbial invasion of the amniotic cavity with Ureaplasma urealyticum is associated with a robust host response in fetal, amniotic, and maternal compartments. Am J Obstet Gynecol179 (1998) 1254Search in Google Scholar
14 Yoon BH, JW Chang, R Romero: Isolation of Ureaplasma urealyticum from the amniotic cavity and adverse outcome in preterm labor. Obstet Gynecol92 (1998) 77Search in Google Scholar
15 Yoon BH, R Romero, M Kim, EC Kim, T Kim, JS Park, JK Jun: Clinical implications of detection of Ureaplasma urealyticum in the amniotic cavity with the polymerase chain reaction. Am J Obstet Gynecol183 (2000) 1130Search in Google Scholar
©2005 by Walter de Gruyter Berlin New York
Articles in the same Issue
- Global approach to perinatal medicine: functional genomics and proteomics
- Fetal and maternal Doppler velocimetry and cytokines in high-risk pregnancy
- IL-8 concentrations in maternal serum, amniotic fluid and cord blood in relation to different pathogens within the amniotic cavity
- Placental pathology and pregnancy outcomes in donor and non-donor oocyte in vitro fertilization pregnancies
- Blink-startle reflex habituation in 30–34-week low-risk fetuses
- Analysis of fetal breathing movements at 30–38 weeks of gestation
- Influence of perinatal factors on hematological variables in umbilical cord blood
- The potential of four-dimensional (4D) ultrasonography in the assessment of fetal awareness
- Early prognostic significance of umbilical cord troponin I in critically ill newborns. Prospective study with a control group
- Is the use of early nasal CPAP associated with lower rates of chronic lung disease and retinopathy of prematurity? Nine years of experience with the Vermont Oxford Neonatal Network
- Fetal growth restriction associated with measles virus infection during pregnancy
- Prenatal diagnosis of hemifacial microsomia by magnetic resonance imaging
- Mega-dose carbamazepine complicating third trimester of pregnancy
- Meconium peritonitis secondary to torsion of fallopian tube cyst and transient central diabetes insipidus in a preterm infant
- Congenital syphilis: β2-microglobulin in cerebrospinal fluid and diagnosis of neurosyphilis in an affected newborn
- Normalization of a severely abnormal ductus venosus Doppler flow velocity waveform in the presence of normal arterial flow parameters
Articles in the same Issue
- Global approach to perinatal medicine: functional genomics and proteomics
- Fetal and maternal Doppler velocimetry and cytokines in high-risk pregnancy
- IL-8 concentrations in maternal serum, amniotic fluid and cord blood in relation to different pathogens within the amniotic cavity
- Placental pathology and pregnancy outcomes in donor and non-donor oocyte in vitro fertilization pregnancies
- Blink-startle reflex habituation in 30–34-week low-risk fetuses
- Analysis of fetal breathing movements at 30–38 weeks of gestation
- Influence of perinatal factors on hematological variables in umbilical cord blood
- The potential of four-dimensional (4D) ultrasonography in the assessment of fetal awareness
- Early prognostic significance of umbilical cord troponin I in critically ill newborns. Prospective study with a control group
- Is the use of early nasal CPAP associated with lower rates of chronic lung disease and retinopathy of prematurity? Nine years of experience with the Vermont Oxford Neonatal Network
- Fetal growth restriction associated with measles virus infection during pregnancy
- Prenatal diagnosis of hemifacial microsomia by magnetic resonance imaging
- Mega-dose carbamazepine complicating third trimester of pregnancy
- Meconium peritonitis secondary to torsion of fallopian tube cyst and transient central diabetes insipidus in a preterm infant
- Congenital syphilis: β2-microglobulin in cerebrospinal fluid and diagnosis of neurosyphilis in an affected newborn
- Normalization of a severely abnormal ductus venosus Doppler flow velocity waveform in the presence of normal arterial flow parameters