Impaired CD14-dependent and independent response of polymorphonuclear leukocytes in preterm infants
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P. Henneke
Abstract
Preterm newborn infants are especially susceptible to Gram-negative sepsis that is associated with a lethality of up to 40%.
Aims: We tested whether polymorphonuclear leukocytes (PMN) from preterm infants exhibit an impaired antibacterial response upon stimulation with lipopolysaccharide (LPS) from Escherichia coli when compared to full term newborns or adults.
Methods: We studied the effect of LPS on the expression of the surface proteins CD11b and CD14 and the secretion of elastase by PMN from preterm infants, term infants and adults ex vivo.
Results: We found a significantly reduced antibacterial activity of PMN from preterm infants upon stimulation with LPS as indicated by low surface expression of the adhesion molecule CD11b and the reduced secretion of PMN elastase. LPS-induced CD11b expression was dependent on binding of LPS to the surface protein CD14 as CD14 antibodies inhibited LPS dependent CD11b upregulation. Furthermore CD14 expression was lower on PMN from preterm infants than from adults. In addition, CD14 independent upregulation of CD11b in response to tumor necrosis factor (TNF-α), N-formyl peptides (FMLP) and phorbol ester (PMA) was impaired.
Conclusion: PMN from preterm infants are distinctly hyporesponsive to LPS, which may explain the predisposition of these children to invasive disease due to gramnegative bacteria.
Copyright © 2003 by Walter de Gruyter GmbH & Co. KG
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Artikel in diesem Heft
- Mode of conception, placental morphology and perinatal outcome of twin gestations
- The effects of birth weight discordance and prematurity on twin neonatal outcome
- Esophageal atresia and tracheoesophageal fistula: the impact of prenatal suspicion on neonatal outcome in a tertiary care center
- Microbial invasion of the amniotic cavity at birth is associated with adverse short-term outcome of preterm infants
- Is treatment with vaginal pessaries an option in patients with a sonographically detected short cervix?
- The effects of intramuscular administration of corticosteroids on the induction of parturition
- Perinatal significance of diagnosing glucose intolerance during pregnancy with portable glucose meter
- Biovar diversity of Ureaplasma urealyticum in amniotic fluid: distribution, intrauterine inflammatory response and pregnancy outcomes
- Comparative study on the expression of cytokine – receptor genes in normal and preeclamptic human placentas using DNA microarrays
- Chorioangioma – new insights into a well-known problem I. Results of a clinical and morphological study of 136 cases
- Chorioangiomas – new insights into a well-known problem II. An immuno-histochemical investigation of 136 cases
- Impaired CD14-dependent and independent response of polymorphonuclear leukocytes in preterm infants
- The left portal vein is the watershed of the fetal venous system
- Congress Calendar
