Startseite External and internal fungal flora of pine sapwood (Pinus sylvestris L.) specimens in above-ground field tests at six different sites in south-west Germany
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External and internal fungal flora of pine sapwood (Pinus sylvestris L.) specimens in above-ground field tests at six different sites in south-west Germany

  • Ulrika Råberg , Christian Brischke , Andreas O. Rapp , Nils O.S. Högberg und Carl Johan Land
Veröffentlicht/Copyright: 1. Januar 2007
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Holzforschung
Aus der Zeitschrift Band 61 Heft 1

Abstract

The occurrence of fungal species on pine sapwood samples obtained from an above-ground field test study was analysed by terminal restriction fragment length polymorphism (T-RFLP), cloning, and sequencing. Samples were taken from eight double-layer set-ups that were exposed to the environment at six different locations in south-west Germany. The occurrence of fungal species was correlated with decay intensity and rot types on one hand, and characteristics of the test sites, such as precipitation, average temperature and height above sea level on the other hand. In total, 62 different fungal species were found based on T-RFLP, cloning and sequencing. Of the 39 species that were found four or more times, 30 were ascomycetes, five were basidiomycetes, and four could not be classified. The most common fungus found in this study was Coniochaeta ligniaria ((Grev.) Cooke), a soft rot fungus that occurred in 87 of 152 samples (57%). No single factor at the test sites seemed to be decisive for the abundance of fungal species or decay intensity. Within the first years of this study, soft rot fungi was found more frequently in pine sapwood specimens than basidiomycetes.

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Corresponding author. Department of Wood Science, P.O. Box 7008, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden

References

Adair, S., Kim, S.H., Breuil, C. (2002) A molecular approach for early monitoring of decay basidiomycetes in wood chips. FEMS Microbiol. Lett.211:117–122.10.1111/j.1574-6968.2002.tb11212.xSuche in Google Scholar PubMed

Allmer, J., Vasiliaukas, R., Ihrmark, K., Stenlid, J., Dahlberg, A. (2005) Wood-inhabiting fungal communities in woody debris of Norway spruce (Picea abies (L.) Karst.) as reflected by sporocarps, mycelial isolation and T-RFLP identification. FEMS Microbiol. Ecol.55:57–67.Suche in Google Scholar

Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W., Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res.25:3389–3402.10.1093/nar/25.17.3389Suche in Google Scholar PubMed PubMed Central

Blanchette, R.A. (2005) Environmental factors influencing microbial growth inside the historic expedition hut of Ross Island, Antarctica. Int. Biodeter. Biodegrad.55:45–53.Suche in Google Scholar

Blanchette, R.A., Nilsson, T., Daniel, G., Abad, A. (1990) Biological degradation of wood. In: Archaeological Wood: Properties, Chemistry, and Preservation. Eds. Rowell, R.M., Barbour, R.J. American Chemical Society, Washington, DC. pp. 141–173.Suche in Google Scholar

Brischke, C., Rapp, A.O. (2005) Experimental approach for service life prediction of wooden materials. In: Proceedings of the 10th International Conference on Durability of Building Materials and Components, Lyon, France, 17–21 April 2005. TT6-198.Suche in Google Scholar

Carey, J.K. (2002a) L-joint trials: Part 1: Observations on the process of colonisation and decay. Document No. IRG/WP 02-20250. International Research Group on Wood Protection, Stockholm, Sweden.Suche in Google Scholar

Carey, J.K. (2002b) L-joint trials: Part 2: The relationship between colonisation by decay fungi and long-term performance. Document No. IRG/WP 02-20251. International Research Group on Wood Protection, Stockholm, Sweden.Suche in Google Scholar

CEN (1989) EN252. Field test method for determining the relative protective effectiveness of wood preservatives in ground contact. European Committee for Standardisation, Brussels, Belgium.Suche in Google Scholar

CEN (1992) EN 335-1. Classification of hazard classes. European Committee for Standardisation, Brussels, Belgium.Suche in Google Scholar

CEN (1993) EN 330. Wood preservatives. Field-test method for determining the relative protective effectiveness of a wood preservative for use under a coating and exposed out-of-ground contact. L-joint method. European Committee for Standardisation, Brussels, Belgium.Suche in Google Scholar

CEN (1996) ENV 12037. Wood preservatives. Field test method for determining the relative effectiveness of a wood preservative exposed out of ground contact. Horizontal lap-joint method. European Committee for Standardisation, Brussels, Belgium.Suche in Google Scholar

CEN (1997) EN 113 Wood preservatives. Test method for determining the protective effectiveness against wood-destroying basidiomycetes. Determination of toxic values. European Committee for Standardisation, Brussels, BelgiumSuche in Google Scholar

Clubbe, C.P. (1980) The colonisation and succession of fungi in wood. Document No. IRG/WP 1107. International Research Group on Wood Protection, Stockholm, Sweden.Suche in Google Scholar

Daniel, G., Nilsson, T. (1998) Development in the study of soft rot and bacterial decay. In: Forest Products Biotechnology. Eds. Bruce, A., Palfreyman, J.W. Taylor and Francis, London. pp. 37–62.Suche in Google Scholar

Deacon, J.W. Fungal Biology. Blackwell Publishing, Oxford, UK, 2006.10.1002/9781118685068Suche in Google Scholar

de Hoog, G.S., Guarro, J., Gene, J., Figueras, M.J. (2000) Atlas of Clinical Fungi. 2nd edition, Vol. 1. Centralbureau voor Schimmelcultures, Utrecht, The Netherlands.Suche in Google Scholar

Dickie, I.A., Xu, B., Koide, R.T. (2002) Vertical niche differentiations of ectomycorrhizal hyphae in soil as shown by T-RFLP analysis. New Phytol.156:527–535.10.1046/j.1469-8137.2002.00535.xSuche in Google Scholar PubMed

Diehl, S.V., McElroy, T.C., Prewitt, M.L. (2004) Development and implementation of a DNA-RFLP database for wood decay and wood associated fungi. Document No. IRG/WP 04-10527. International Research Group on Wood Protection, Stockholm, Sweden.Suche in Google Scholar

Dix, N.J., Webster, J. (1995) Fungal Ecology. Chapman and Hall, London. p. 549.10.1007/978-94-011-0693-1Suche in Google Scholar

Eaton, R.A., Hale, M.D.C. Wood, Decay, Pests and Protection. Chapman and Hall, London, 1993.Suche in Google Scholar

Findley, W.P.K. (1965) Ecology of wood-destroying and wood-inhabiting fungi. Holz Organism.1:199–211.Suche in Google Scholar

Hawksworth, D.L., Yip, H.Y. (1981) Coniohaeta angustispora sp. nov. from roots in Australia, with a key to species known in culture. Aust. J. Bot.29:377–384.Suche in Google Scholar

Högberg, N., Land, C.J. (2004) Identification of Serpula lacrymans and other decay fungi in construction timber by sequencing of ribosomal DNA – A practical approach. Holzforschung58:199–204.10.1515/HF.2004.030Suche in Google Scholar

Ihaka, R., Gentleman, R. (1996) A language for data analysis and graphics. J. Comput. Graph. Stat.5:299–314.Suche in Google Scholar

Jankowiak, R. (2005) Fungi associated with Ips typographus on Picea abies in southern Poland and their succession into the phloem and sapwood of beetle-infested trees and logs. For. Pathol.35:37–55.10.1111/j.1439-0329.2004.00395.xSuche in Google Scholar

Jasalavich, C.A., Ostrofsky, A., Jellison, J. (2000) Detection and identification of decay fungi in spruce wood by restriction fragment length polymorphism analysis of amplified genes encoding rRNA. Appl. Environ. Microbiol.66:4725–4734.10.1128/AEM.66.11.4725-4734.2000Suche in Google Scholar PubMed PubMed Central

Jellison, J., Jasalavich, C., Ostrofsky, A. (2003) Detecting and identifying wood decay fungi using DNA analysis. In: Wood Deterioration and Preservation: Advances in our Changing World. Eds. Goodell, B., Nicholas, D.D., Schultz, T.P. American Chemical Society, Washington, DC. pp. 346–357.10.1021/bk-2003-0845.ch020Suche in Google Scholar

Käärik, A. (1974) Decomposition of wood. In: Biology of Plant Litter Decomposition. Eds. Dickinson, C.H., Pugh, G.J.F. Academic Press, New York. pp. 129–174.10.1016/B978-0-12-215001-2.50011-8Suche in Google Scholar

Käärik, A., Rennerfelt, E. (1957) [Investigation of the fungal flora of spruce and pine stumps] (in Swedish). Meddelande från Statens Skogsforskningsinstitut 47:7.Suche in Google Scholar

Kitts, C.L. (2001) Terminal restriction fragment patterns: A tool for comparing microbial communities and assessing community dynamics. Curr. Iss. Intest. Microbiol.2:17–25.Suche in Google Scholar

Levy J.F. (1982) The place of basidiomycetes in the decay of wood in contact with the ground. In: Decomposer Basidiomycetes: Their Biology and Ecology. Eds. Frankland, J., Hedger, J.N., Swift, M.J. Cambridge University Press, Cambridge, UK. pp. 161–178.Suche in Google Scholar

Littell, R.C., Milliken, G.A., Stroup, W.W., Wolfinger, R.D. SAS System for Mixed Models. SAS Institute, Cary, NC, USA, 1996.Suche in Google Scholar

Moreth, U., Schmidt, O. (2000) Identification of indoor rot fungi by taxon-specific priming polymerase chain reaction. Holzforschung 54:1–8.10.1515/HF.2000.001Suche in Google Scholar

Olsson, U. Generalized Linear Models: An Applied Approach. Studentlitteratur, Lund, Sweden, 2002.Suche in Google Scholar

Råberg, U., Högberg, N., Land, C.J. (2004) Identification of brown rot fungi on wood in above ground conditions by PCR, T-RFLP and sequencing. Document No. IRG/WP 04-10512. International Research Group on Wood Protection, Stockholm, Sweden.Suche in Google Scholar

Råberg, U., Högberg, N.O.S., Land, C.J. (2005) Detection and species discrimination using rDNA T-RFLP for identification of wood decay fungi. Holzforschung59:696–702.10.1515/HF.2005.111Suche in Google Scholar

Rapp, A.O., Augusta, U. (2004) The full guideline for the “double layer test method”– A field test method for determining the durability of wood out of ground. Document No. IRG/WP 04-20290. International Research Group on Wood Protection, Stockholm, Sweden.Suche in Google Scholar

Ray, M.J., Dickinson, D.J., Buck, M. (2004) Aureobasidium or Hormonema? A genetic approach. Document No. IRG/WP 04-10529. International Research Group on Wood Protection, Stockholm, Sweden.Suche in Google Scholar

Rayner, A.D.M., Boddy, L. Fungal Decomposition of Wood: Its Biology and Ecology. John Wiley & Sons, Chichester, UK, 1988.Suche in Google Scholar

Savonmäki, S., Salonen, A., Ruokola, A.-L. (1992) Microfungi isolated from wood of Scots pine in Finland. Karstenia32:65–70.10.29203/ka.1992.293Suche in Google Scholar

SAS (2004) SAS/Stat User's Guide, Version 9. SAS Institute, Cary, NC, USA.Suche in Google Scholar

Schmidt, O. (2000) Molecular methods for the characterization and identification of the dry rot fungus Serpula lacrymans. Holzforschung54:221–228.10.1515/HF.2000.038Suche in Google Scholar

Schmidt, O. Wood and Tree Fungi – Biology, Damage, Protection, and Use. Springer-Verlag, Berlin, 2006.Suche in Google Scholar

Schmidt, O., Moreth, U. (1999) Identification of the dry rot fungus, Serpula lacrymans, and the wild Merulius, S. himantioides, by amplified ribosomal DNA restriction analysis (ARDRA). Holzforschung53:123–128.Suche in Google Scholar

Schmidt, O., Moreth, U. (2000) Species-specific PCR primers in the rDNA-ITS region as a diagnostic tool for Serpula lacrymans. Mycol. Res.104:69–72.10.1017/S0953756299001562Suche in Google Scholar

Schmidt, O., Moreth, U. (2003) Molecular identity of species and isolates of internal pore fungi Antrodia spp. and Oligoporus placenta. Holzforschung57:120–126.Suche in Google Scholar

Schmidt, O., Grimm, K., Moreth, U. (2002) Molecular identity of species and isolates of the Coniophora cellar fungi. Holzforschung56:563–571.10.1515/HF.2002.086Suche in Google Scholar

Published Online: 2007-01-01
Published in Print: 2007-01-01

©2007 by Walter de Gruyter Berlin New York

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