Article
Licensed
Unlicensed
Requires Authentication
Spurious increase in serum chromogranin A: the role of heterophilic antibodies
-
Luca Giovanella
Published/Copyright:
July 29, 2010
Abstract
Background: The aim of the study was to examine the prevalence of heterophilic antibody (HAb) interference on serum chromogranin A (CgA) measurements.
Methods: Serum CgA was measured in 672 serum samples before and following incubation in heterophilic antibody blocking tubes (HBTs). Results showing more than 3 and 5 SD percentage difference from the original result were considered to be affected by possible or definite HAb interference, respectively. All possible interferences were confirmed or excluded by non-linear or linear dilution tests.
Results: Following HBT treatment, CgA concentrations fell >3 SD percent compared with the original result in seven specimens, six of which also showed non-linear dilution (0.9%). A clinically relevant increase in serum CgA occurred in five patients (0.7%). No samples showed a significant increase of >3 SD percent following HBT treatment.
Conclusions: Interference from HAb occurred in up to 0.9% of sera tested for serum CgA. If an increased CgA result does not fit the clinical picture, pretreatment of serum in HBT tubes may prevent unwarranted investigations or therapies.
Clin Chem Lab Med 2010;48:1497–9.
Received: 2010-3-24
Accepted: 2010-5-3
Published Online: 2010-07-29
Published in Print: 2010-10-01
©2010 by Walter de Gruyter Berlin New York
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Editorial
- Darwinian evolution or regression? The fate of laboratory professionals
- Review
- Automated reticulocyte counting: state of the art and clinical applications in the evaluation of erythropoiesis
- Minireview
- Laboratory diagnostics in acute poisoning: critical overview
- Opinion Papers
- On the origins of physicians: Darwinian or Lamarckian evolution?
- Observed, unknown distributions of clinical chemical quantities should be considered to be log-normal: a proposal
- Guidelines and Recommendations
- Development and description of GETT: a Genetic testing Evidence Tracking Tool
- Genetics and Molecular Diagnostics
- DNA sequencing errors in molecular diagnostics of filamin myopathy
- High resolution melting for the identification of mutations in the iron responsive element of the ferritin light chain gene
- General Clinical Chemistry and Laboratory Medicine
- Detection of serum free light chains: the problem with antigen excess
- Hepcidin concentrations and iron homeostasis in preeclampsia
- Automated assay for non-transferrin-bound iron in serum samples
- A simple liquid chromatography-tandem mass spectrometry method for urinary free cortisol analysis: suitable for routine purpose
- Evaluation of a multiplex assay for adipokine concentrations in obese children
- The use of serial patient blood gas, electrolyte and glucose results to derive biologic variation: a new tool to assess the acceptability of intensive care unit testing
- Point-of-care determination of neonatal bilirubin with the blood gas analyzer RapidLab 1265
- The effect of pre-analytical variables on light transmittance aggregometry in citrated platelet-rich plasma from healthy subjects
- Cancer Diagnostics
- Clinical significance of serum macrophage-colony stimulating factor (M-CSF) in esophageal cancer patients and its comparison with classical tumor markers
- VKORC1 haplotypes influence the performance characteristics of PIVKAII for screening of hepatocellular carcinoma
- Serum soluble tumour necrosis factor receptor type I concentrations independently predict prognosis in patients with breast cancer
- Variations in systemic biomarkers of oxidative/nitrosative stress and DNA damage before and during the consequent two cycles of chemotherapy in breast cancer patients
- Spurious increase in serum chromogranin A: the role of heterophilic antibodies
- Infectious Diseases
- Identification and evaluation of a new nucleic acid amplification test target for specific detection of Mycobacterium tuberculosis
- Application of an oligonucleotide microarray-based nano-amplification technique for the detection of fungal pathogens
- Liquid chromatography-tandem mass spectrometry method for simultaneous quantification of four triazole antifungal agents in human plasma
- Evaluation of two sirolimus assays using the ARCHITECT-i1000® CMIA or RxL® ACMIA methods in comparison with the IMx® MEIA method
- Widespread use of point-of-care testing is irreconcilable with the present-day quest for safety
- Congress Abstracts
- 3rd Annual Congress of the Austrian Society for Laboratory Medicine and Clinical Chemistry Salzburg, Austria, 27-30 October 2010
Articles in the same Issue
- Editorial
- Darwinian evolution or regression? The fate of laboratory professionals
- Review
- Automated reticulocyte counting: state of the art and clinical applications in the evaluation of erythropoiesis
- Minireview
- Laboratory diagnostics in acute poisoning: critical overview
- Opinion Papers
- On the origins of physicians: Darwinian or Lamarckian evolution?
- Observed, unknown distributions of clinical chemical quantities should be considered to be log-normal: a proposal
- Guidelines and Recommendations
- Development and description of GETT: a Genetic testing Evidence Tracking Tool
- Genetics and Molecular Diagnostics
- DNA sequencing errors in molecular diagnostics of filamin myopathy
- High resolution melting for the identification of mutations in the iron responsive element of the ferritin light chain gene
- General Clinical Chemistry and Laboratory Medicine
- Detection of serum free light chains: the problem with antigen excess
- Hepcidin concentrations and iron homeostasis in preeclampsia
- Automated assay for non-transferrin-bound iron in serum samples
- A simple liquid chromatography-tandem mass spectrometry method for urinary free cortisol analysis: suitable for routine purpose
- Evaluation of a multiplex assay for adipokine concentrations in obese children
- The use of serial patient blood gas, electrolyte and glucose results to derive biologic variation: a new tool to assess the acceptability of intensive care unit testing
- Point-of-care determination of neonatal bilirubin with the blood gas analyzer RapidLab 1265
- The effect of pre-analytical variables on light transmittance aggregometry in citrated platelet-rich plasma from healthy subjects
- Cancer Diagnostics
- Clinical significance of serum macrophage-colony stimulating factor (M-CSF) in esophageal cancer patients and its comparison with classical tumor markers
- VKORC1 haplotypes influence the performance characteristics of PIVKAII for screening of hepatocellular carcinoma
- Serum soluble tumour necrosis factor receptor type I concentrations independently predict prognosis in patients with breast cancer
- Variations in systemic biomarkers of oxidative/nitrosative stress and DNA damage before and during the consequent two cycles of chemotherapy in breast cancer patients
- Spurious increase in serum chromogranin A: the role of heterophilic antibodies
- Infectious Diseases
- Identification and evaluation of a new nucleic acid amplification test target for specific detection of Mycobacterium tuberculosis
- Application of an oligonucleotide microarray-based nano-amplification technique for the detection of fungal pathogens
- Liquid chromatography-tandem mass spectrometry method for simultaneous quantification of four triazole antifungal agents in human plasma
- Evaluation of two sirolimus assays using the ARCHITECT-i1000® CMIA or RxL® ACMIA methods in comparison with the IMx® MEIA method
- Widespread use of point-of-care testing is irreconcilable with the present-day quest for safety
- Congress Abstracts
- 3rd Annual Congress of the Austrian Society for Laboratory Medicine and Clinical Chemistry Salzburg, Austria, 27-30 October 2010
