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Variations in systemic biomarkers of oxidative/nitrosative stress and DNA damage before and during the consequent two cycles of chemotherapy in breast cancer patients

  • Pinar Atukeren , Berna Yavuz , Hilal Oguz Soydinc , Sevim Purisa , Hakan Camlica , M. Koray Gumustas and Ibrahim Balcioglu
Published/Copyright: July 7, 2010
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Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 48 Issue 10

Abstract

Background: Previous studies have suggested the importance of redox regulation in carcinogenesis. The aim of this study was to evaluate the prognostic role of altered redox homeostasis and oxidative DNA damage in patients with breast carcinoma before and during two cycles of chemotherapy.

Methods: This study included 30 patients whose serum samples were obtained on admission before treatment, and after the first and second cycles of chemotherapy, and 20 controls. We investigated serum total antioxidant status (TAS), thiobarbituric acid reacting substances (TBARS), total nitrite/nitrate (NOx), nitrotyrosine (NT), and 8-hydroxydeoxy-guanosine (8-OHdG), as well as antioxidant enzyme activities, such as glutathione peroxidase (GPx), glutathione reductase (GRx).

Results: TBARS, NOx, NT and 8-OHdG concentrations were significantly increased, while antioxidant enzyme activities and TAS were significantly decreased in patients when compared to controls. A concurrent increase in TBARS, NOx, NT, and 8-OHdG and a decrease in antioxidant enzyme activities and TAS were also seen after chemotherapy. No difference was observed in the second cycle of chemotherapy when compared with the first course.

Conclusions: In conclusion, decreased activities of these antioxidant enzymes and low TAS concentrations observed in our study might be due to the depletion of the antioxidant defense system to combat the free radical storm produced by chemotherapy. We suggest that the increased 8-OHdG and other oxidative/nitrosative stress products that we have measured in breast cancer patients may be prognostic risk factors for the magnitude of oxidation in serum.

Clin Chem Lab Med 2010;48:1487–95.

Keywords: 8-hydroxydeoxyguanosine; breast cancer; chemotherapy; nitrosative stress; oxidative stress
Corresponding author: Pinar Atukeren, PhD, Ataturk Caddesi. Akdeniz Sitesi A/1 Blok D: 7 34742 Kozyatagı, Istanbul, Turkey Phone: +90 212 414 30 00/23004, Fax: +90 212 414 30 56,
Received: 2010-1-28
Accepted: 2010-4-14
Published Online: 2010-07-7
Published in Print: 2010-10-01

©2010 by Walter de Gruyter Berlin New York

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https://doi.org/10.1515/CCLM.2010.249
Keywords for this article
8-hydroxydeoxyguanosine; breast cancer; chemotherapy; nitrosative stress; oxidative stress

Articles in the same Issue

  1. Editorial
  2. Darwinian evolution or regression? The fate of laboratory professionals
  3. Review
  4. Automated reticulocyte counting: state of the art and clinical applications in the evaluation of erythropoiesis
  5. Minireview
  6. Laboratory diagnostics in acute poisoning: critical overview
  7. Opinion Papers
  8. On the origins of physicians: Darwinian or Lamarckian evolution?
  9. Observed, unknown distributions of clinical chemical quantities should be considered to be log-normal: a proposal
  10. Guidelines and Recommendations
  11. Development and description of GETT: a Genetic testing Evidence Tracking Tool
  12. Genetics and Molecular Diagnostics
  13. DNA sequencing errors in molecular diagnostics of filamin myopathy
  14. High resolution melting for the identification of mutations in the iron responsive element of the ferritin light chain gene
  15. General Clinical Chemistry and Laboratory Medicine
  16. Detection of serum free light chains: the problem with antigen excess
  17. Hepcidin concentrations and iron homeostasis in preeclampsia
  18. Automated assay for non-transferrin-bound iron in serum samples
  19. A simple liquid chromatography-tandem mass spectrometry method for urinary free cortisol analysis: suitable for routine purpose
  20. Evaluation of a multiplex assay for adipokine concentrations in obese children
  21. The use of serial patient blood gas, electrolyte and glucose results to derive biologic variation: a new tool to assess the acceptability of intensive care unit testing
  22. Point-of-care determination of neonatal bilirubin with the blood gas analyzer RapidLab 1265
  23. The effect of pre-analytical variables on light transmittance aggregometry in citrated platelet-rich plasma from healthy subjects
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  26. VKORC1 haplotypes influence the performance characteristics of PIVKAII for screening of hepatocellular carcinoma
  27. Serum soluble tumour necrosis factor receptor type I concentrations independently predict prognosis in patients with breast cancer
  28. Variations in systemic biomarkers of oxidative/nitrosative stress and DNA damage before and during the consequent two cycles of chemotherapy in breast cancer patients
  29. Spurious increase in serum chromogranin A: the role of heterophilic antibodies
  30. Infectious Diseases
  31. Identification and evaluation of a new nucleic acid amplification test target for specific detection of Mycobacterium tuberculosis
  32. Application of an oligonucleotide microarray-based nano-amplification technique for the detection of fungal pathogens
  33. Liquid chromatography-tandem mass spectrometry method for simultaneous quantification of four triazole antifungal agents in human plasma
  34. Evaluation of two sirolimus assays using the ARCHITECT-i1000® CMIA or RxL® ACMIA methods in comparison with the IMx® MEIA method
  35. Widespread use of point-of-care testing is irreconcilable with the present-day quest for safety
  36. Congress Abstracts
  37. 3rd Annual Congress of the Austrian Society for Laboratory Medicine and Clinical Chemistry Salzburg, Austria, 27-30 October 2010
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