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Performance analysis of the ARCHITECT anti-cyclic citrullinated peptide antibody in the diagnosis of rheumatoid arthritis
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Sang Mee Hwang
Veröffentlicht/Copyright:
4. Dezember 2009
Abstract
Background: Anti-cyclic citrullinated peptide (CCP) antibody is emerging as an important diagnostic marker for rheumatoid arthritis (RA). We evaluated the analytical and diagnostic performance of the ARCHITECT anti-CCP (Abbott Diagnostics), a new fully automated chemiluminescent microparticle immunoassay.
Methods: Serum samples from 69 patients with RA and 86 non-RA patients were used to evaluate the performance of the ARCHITECT anti-CCP assay, and the results were compared with those of EliA CCP (Phadia). The optimal cut-off value was calculated using receiver operating characteristic (ROC) curve analysis.
Results: Within-run and total imprecision (%CV) of the ARCHITECT anti-CCP were <6% and good linearity was observed over the claimed range. The areas under the ROC curves for the ARCHITECT anti-CCP and EliA CCP were 0.90 and 0.89, respectively. The sensitivity and specificity were 76.8% and 95.3% for the ARCHITECT anti-CCP and 78.3% and 95.3% for EliA CCP using the manufacturer's cut-off thresholds. Both assays showed sensitivity of 84.1% and specificity of 94.2% using the optimal cut-off values.
Conclusions: The analytical performance of the ARCHITECT anti-CCP was satisfactory and diagnostic performance was comparable to that of EliA CCP. The use of optimal cut-off thresholds can yield higher sensitivity with minimal loss of specificity.
Clin Chem Lab Med 2010;48:225–30.
Keywords: analytical performance; anti-cyclic citrullinated peptide; diagnostic performance; rheumatoid arthritis
Received: 2009-8-7
Accepted: 2009-10-13
Published Online: 2009-12-04
Published in Print: 2010-02-01
©2010 by Walter de Gruyter Berlin New York
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Schlagwörter für diesen Artikel
analytical performance;
anti-cyclic citrullinated peptide;
diagnostic performance;
rheumatoid arthritis
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- Elevated serum γ-glutamyltransferase activity is associated with increased risk of mortality, incident type 2 diabetes, cardiovascular events, chronic kidney disease and cancer – a narrative review
- Minireview
- Artificial intelligence for diagnostic purposes: principles, procedures and limitations
- General Clinical Chemistry and Laboratory Medicine
- Association between serum alkaline phosphatase and C-reactive protein in the United States National Health and Nutrition Examination Survey 2005–2006
- Association between serum uric acid and non-alcoholic fatty liver disease in Korean adults
- Plasma osteopontin concentrations in preeclampsia – is there an association with endothelial injury?
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