Article
Licensed
Unlicensed
Requires Authentication
Heat shock protein 27 is over-expressed in tumor tissues and increased in sera of patients with gastric adenocarcinoma
-
Qiaojia Huang
Published/Copyright:
December 7, 2009
Abstract
Background: In a previous study, we found that heat shock protein 27 (HSP27) was over-expressed in gastric adenocarcinoma (GA) tissue. In this study, our goal was to further verify the expression profile of HSP27 in patients with GA.
Methods: Western blot and immunohistochemistry were employed to determine HSP27 expression in 50 paired tumor and adjacent normal tissue. ELISA was used to quantify serum HSP27 concentrations in the same 50 GA patients and 50 healthy individuals.
Results: Compared to adjacent normal tissues, HSP27 was over-expressed in 25 (50%, p=0.000) and 24 (48%, p=0.000) cases of GA tissue by Western blot and immunohistochemistry, respectively. ELISA revealed significantly higher serum concentrations of HSP27 in patients with GA patients (mean=986 pg/mL) compared to healthy individuals (mean=573 pg/mL) (p=0.003). In addition, infection with Helicobacter pylori (HP) in healthy individuals was associated with increased expression of HSP27 in both gastric mucosa and serum.
Conclusions: These data suggest that HSP27 is over-expressed in GA tissue and serum concentrations of HSP27 are increased in patients with GA. Over-expression of HSP27 may indicate a gastric malignant/infectious process. The detection of serum HSP27 concentrations by ELISA may be useful for screening for GA.
Clin Chem Lab Med 2010;48:263–9.
Received: 2009-8-6
Accepted: 2009-10-12
Published Online: 2009-12-07
Published in Print: 2010-02-01
©2010 by Walter de Gruyter Berlin New York
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Review
- Elevated serum γ-glutamyltransferase activity is associated with increased risk of mortality, incident type 2 diabetes, cardiovascular events, chronic kidney disease and cancer – a narrative review
- Minireview
- Artificial intelligence for diagnostic purposes: principles, procedures and limitations
- General Clinical Chemistry and Laboratory Medicine
- Association between serum alkaline phosphatase and C-reactive protein in the United States National Health and Nutrition Examination Survey 2005–2006
- Association between serum uric acid and non-alcoholic fatty liver disease in Korean adults
- Plasma osteopontin concentrations in preeclampsia – is there an association with endothelial injury?
- Determination of globotriaosylceramide in plasma and urine by mass spectrometry
- Effect of inflammation induced by prolonged exercise on circulating erythroid progenitors and markers of erythropoiesis
- The Beckman DxI 800 prolactin assay demonstrates superior specificity for monomeric prolactin
- Stability of cerebrospinal fluid/serum glucose ratio and cerebrospinal fluid lactate concentrations over 24 h: analysis of repeated measurements
- O-β-N-acetyl-D-glucosaminidase in erythrocytes of Italian air force acrobatic pilots
- Validation and Outcome Studies
- Experimental validation of specificity of the squamous cell carcinoma antigen-immunoglobulin M (SCCA-IgM) assay in patients with cirrhosis
- Performance analysis of the ARCHITECT anti-cyclic citrullinated peptide antibody in the diagnosis of rheumatoid arthritis
- Reference Values and Biological Variations
- Applicability of common reference intervals for serum creatinine concentrations to the Croatian population
- Laboratory reference intervals during pregnancy, delivery and the early postpartum period
- Determination of serum holotranscobalamin concentrations with the AxSYM active B12 assay: cut-off point evaluation in the clinical laboratory
- Definition of reference ranges for the platelet distribution width (PDW): a local need
- Cancer Diagnostics
- Mean leukocyte telomere length and risk of incident colorectal carcinoma in women: a prospective, nested case-control study
- Heat shock protein 27 is over-expressed in tumor tissues and increased in sera of patients with gastric adenocarcinoma
- Soluble mesothelin related peptides (SMRP) and osteopontin as protein biomarkers for malignant mesothelioma: analytical validation of ELISA based assays and characterization at mRNA and protein levels
- A novel sensitive immunoassay by nucleic acid barcode dot and its application in the detection of prostate-specific antigen
- Prostate cancer screening: clinical impact of WHO calibration of Beckman Coulter Access® prostate-specific antigen assays
- Infectious Diseases
- Use of flow cytometry (Sysmex® UF-100) to screen for positive urine cultures: in search for the ideal cut-off
- Letters to the Editor
- Improving laboratory efficiencies through significant time reduction in the preanalytical phase
- Comparison of the Vitek 2 system with the CLSI broth microdilution, disk diffusion, and sterol quantitation methods for determining fluconazole susceptibility against Candida spp.
Keywords for this article
ELISA;
gastric adenocarcinomas;
heat shock protein 27;
immunohistochemistry;
Western blot
Articles in the same Issue
- Review
- Elevated serum γ-glutamyltransferase activity is associated with increased risk of mortality, incident type 2 diabetes, cardiovascular events, chronic kidney disease and cancer – a narrative review
- Minireview
- Artificial intelligence for diagnostic purposes: principles, procedures and limitations
- General Clinical Chemistry and Laboratory Medicine
- Association between serum alkaline phosphatase and C-reactive protein in the United States National Health and Nutrition Examination Survey 2005–2006
- Association between serum uric acid and non-alcoholic fatty liver disease in Korean adults
- Plasma osteopontin concentrations in preeclampsia – is there an association with endothelial injury?
- Determination of globotriaosylceramide in plasma and urine by mass spectrometry
- Effect of inflammation induced by prolonged exercise on circulating erythroid progenitors and markers of erythropoiesis
- The Beckman DxI 800 prolactin assay demonstrates superior specificity for monomeric prolactin
- Stability of cerebrospinal fluid/serum glucose ratio and cerebrospinal fluid lactate concentrations over 24 h: analysis of repeated measurements
- O-β-N-acetyl-D-glucosaminidase in erythrocytes of Italian air force acrobatic pilots
- Validation and Outcome Studies
- Experimental validation of specificity of the squamous cell carcinoma antigen-immunoglobulin M (SCCA-IgM) assay in patients with cirrhosis
- Performance analysis of the ARCHITECT anti-cyclic citrullinated peptide antibody in the diagnosis of rheumatoid arthritis
- Reference Values and Biological Variations
- Applicability of common reference intervals for serum creatinine concentrations to the Croatian population
- Laboratory reference intervals during pregnancy, delivery and the early postpartum period
- Determination of serum holotranscobalamin concentrations with the AxSYM active B12 assay: cut-off point evaluation in the clinical laboratory
- Definition of reference ranges for the platelet distribution width (PDW): a local need
- Cancer Diagnostics
- Mean leukocyte telomere length and risk of incident colorectal carcinoma in women: a prospective, nested case-control study
- Heat shock protein 27 is over-expressed in tumor tissues and increased in sera of patients with gastric adenocarcinoma
- Soluble mesothelin related peptides (SMRP) and osteopontin as protein biomarkers for malignant mesothelioma: analytical validation of ELISA based assays and characterization at mRNA and protein levels
- A novel sensitive immunoassay by nucleic acid barcode dot and its application in the detection of prostate-specific antigen
- Prostate cancer screening: clinical impact of WHO calibration of Beckman Coulter Access® prostate-specific antigen assays
- Infectious Diseases
- Use of flow cytometry (Sysmex® UF-100) to screen for positive urine cultures: in search for the ideal cut-off
- Letters to the Editor
- Improving laboratory efficiencies through significant time reduction in the preanalytical phase
- Comparison of the Vitek 2 system with the CLSI broth microdilution, disk diffusion, and sterol quantitation methods for determining fluconazole susceptibility against Candida spp.
