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Use of the TLX ultracentrifuge for the isolation of different density lipoproteins and effects of freeze/thawing of human plasma before ultracentrifugation
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Valentine Charlton-Menys
Published/Copyright:
July 7, 2008
Abstract
Background: Isolation of different density lipoproteins by ultracentrifugation can require lengthy centrifugation times and freeze/thawing of plasma may influence recovery.
Methods: We isolated a range of lipoproteins using a preparative ultracentrifuge and the TLX micro-ultracentrifuge and determined the effect of freeze/thawing of plasma beforehand.
Results: In fresh plasma, there was no significant difference in results for small-dense low-density lipoprotein apolipoprotein B (LDL apoB) (density >1.044 g/mL) or cholesterol at density >1.006 g/mL. Freeze/thawing had no effect on closely correlated results for small-dense LDL apoB (r=0.85; p<0.0001) or high-density lipoprotein (r=0.93; p<0.0001).
Conclusions: The TLX micro-ultracentrifuge is a reliable alternative to the preparative ultracentrifuge and freeze/thawing has only a small effect on small-dense LDL apoB or high-density lipoprotein cholesterol.
Clin Chem Lab Med 2008;46:1285–8.
Keywords: high-density lipoprotein (HDL); lipoproteins; non-HDL cholesterol; small-dense low-density lipoprotein; ultracentrifugation; very low-density lipoprotein
Received: 2008-3-17
Accepted: 2008-5-8
Published Online: 2008-07-7
Published in Print: 2008-09-01
©2008 by Walter de Gruyter Berlin New York
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Keywords for this article
high-density lipoprotein (HDL);
lipoproteins;
non-HDL cholesterol;
small-dense low-density lipoprotein;
ultracentrifugation;
very low-density lipoprotein
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