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Reference intervals for thyreotropin and thyroid hormones for healthy adults based on the NOBIDA material and determined using a Modular E170®

  • Lennart Friis-Hansen and Linda Hilsted
Published/Copyright: August 5, 2008
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Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 46 Issue 9

Abstract

Background: The aim of the present study was to establish Nordic reference intervals for thyreotropin (TSH) and the thyroid hormones in heparinized plasma.

Methods: We used 489 heparinized blood samples, collected in the morning, from the Nordic NOBIDA reference material, from healthy adults without medication. TSH, thyroxine, free thyroxine, triiodothyronine, free triiodothyronine, and thyroglobulin antibodies (Tg-ab) were measured using assays for Roche Modular E170® and thyroid peroxidase antibodies (TPO-ab) were measured using the Brahms Kryptor assay.

Results: The measured concentrations for the thyroid hormones, but not TSH, followed a Gaussian distribution. There were more TPO-ab and Tg-ab positive women than men. After exclusion of the TPO-ab and the Tg-ab positive individuals, the reference interval TSH was 0.64 (0.61–0.72) to 4.7 (4.4–5.0) mIU/L. The exclusion of these ab-positive samples also minimized the differences in TSH concentrations between the sexes and the different Nordic countries. For the thyroid hormones, there were only minor differences between the reference intervals between the Nordic populations and between men and women. These reference intervals were unaffected by removal of the TPO-ab and TG-ab positive samples.

Conclusions: The upper limit of the TSH reference interval in our study is high compared to some other recent reports. This could be due to blood sampling in the morning. Furthermore, the Roche platform gives slightly higher results than other platforms. The number and distribution of the samples in the NOBIDA material makes it suitable for the determination of hormone Nordic reference intervals.

Clin Chem Lab Med 2008;46:1305–12.

Keywords: immunoassay; plasma; thyreotropin; thyroglobulin antibody; thyroid hormones; thyroid peroxidase antibody
Corresponding author: Lennart Friis-Hansen, MD, Department of Clinical Biochemistry, KB3011 Rigshospitalet, 2100 Copenhagen, Denmark Phone: +45-35-458726, Fax: +45-35-454640,
Received: 2008-1-8
Accepted: 2008-5-19
Published Online: 2008-08-5
Published in Print: 2008-09-01

©2008 by Walter de Gruyter Berlin New York

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https://doi.org/10.1515/CCLM.2008.258
Keywords for this article
immunoassay; plasma; thyreotropin; thyroglobulin antibody; thyroid hormones; thyroid peroxidase antibody

Articles in the same Issue

  1. Review
  2. Application of spin label electron paramagnetic resonance in the diagnosis and prognosis of cancer and sepsis
  3. Opinion Papers
  4. Local regression: a new approach for measurement system comparison analysis
  5. Accuracy in clinical chemistry – who will kiss Sleeping Beauty awake?
  6. A practical approach to instrument selection, evaluation, basic financial management and implementation in pathology and research
  7. Genetics and Molecular Diagnostics
  8. Fetal hemoglobin: assessment of glycation and acetylation status by electrospray ionization mass spectrometry
  9. Preliminary validation of real-time PCR assays for the identification of Yersinia pestis
  10. Biochemical and molecular investigation of two Korean patients with glycogen storage disease type III
  11. Are microarrays useful in the screening of ABCA4 mutations in Italian patients affected by macular degenerations?
  12. General Clinical Chemistry and Laboratory Medicine
  13. Quantification of vascular endothelial growth factor, interleukin-8, and basic fibroblast growth factor in plasma of cancer patients and healthy volunteers – comparison of ELISA and microsphere-based multiplexed immunoassay
  14. Low serum vitamin B12 is associated with recurrent pregnancy loss in Syrian women
  15. Allantoin as a marker of oxidative stress in human erythrocytes
  16. Diagnostic performance of rapid tests for detection of fecal calprotectin and lactoferrin and their ability to discriminate inflammatory from irritable bowel syndrome
  17. Measurement of mycophenolic acid in plasma or serum by a commercial enzyme inhibition technique in comparison with a high performance liquid chromatography method
  18. Use of the TLX ultracentrifuge for the isolation of different density lipoproteins and effects of freeze/thawing of human plasma before ultracentrifugation
  19. Comprehensive profiling of the human circulating endocannabinoid metabolome: clinical sampling and sample storage parameters
  20. Reference Values and Biological Variations
  21. Reference ranges for neuroprotein S-100B: from infants to adolescents
  22. Variability in longitudinal cerebrospinal fluid tau and phosphorylated tau measurements
  23. Reference intervals for thyreotropin and thyroid hormones for healthy adults based on the NOBIDA material and determined using a Modular E170®
  24. Influence of acute physical exercise on emerging muscular biomarkers
  25. Guidelines and Recommendations
  26. Trueness verification of actual creatinine assays in the European market demonstrates a disappointing variability that needs substantial improvement. An international study in the framework of the EC4 creatinine standardization working group
  27. Letters to the Editor
  28. What's in a name? Standardisation of HbA1c: a response
  29. Choice of sample for the analysis of circulating angiogenic cytokines
  30. Plasminogen activator inhibitor-1 (PAI-1), toll-like receptor 4 (TLR4), factor II (FII), FXIII and fibrinogen polymorphisms are not associated with the prevalence of sudden death due to ventricular fibrillation during myocardial infarction
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  39. SIBioC 2008 40th National Congress of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology, October 28–31, 2008, Rimini, Italy
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