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Application of spin label electron paramagnetic resonance in the diagnosis and prognosis of cancer and sepsis
-
Andrey Gurachevsky
Published/Copyright:
July 21, 2008
Abstract
Diagnostic medicine has seen significant changes during the past decade. The emergence of proteomics and genomics has significantly increased our understanding of disease. These fields have also revealed the vast array of proteins that are expressed in various disease processes, such as cancer. Measurement of these unique proteins expressed in certain diseases may offer diagnostic clues or allow patient prognosis to be assessed. Another approach is to measure the effects that these ligands have on the structure and function of albumin. Albumin is known to play an important role in modulating the serum concentrations of various proteins produced by tumor cells. In this review, we introduce the reader to the technique of spin labeling followed by electron paramagnetic resonance spectroscopy. This method is a powerful tool for evaluating the structural and functional changes that can occur to albumin following the binding of various ligands. We describe the utility of this technique for the diagnosis of cancer and sepsis, as well as some other novel potential applications.
Clin Chem Lab Med 2008;46:1203–10.
Keywords: albumin; cancer; diagnostic medicine; electron paramagnetic resonance spectroscopy; sepsis
Received: 2008-3-14
Accepted: 2008-5-23
Published Online: 2008-07-21
Published in Print: 2008-09-01
©2008 by Walter de Gruyter Berlin New York
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Keywords for this article
albumin;
cancer;
diagnostic medicine;
electron paramagnetic resonance spectroscopy;
sepsis
Articles in the same Issue
- Review
- Application of spin label electron paramagnetic resonance in the diagnosis and prognosis of cancer and sepsis
- Opinion Papers
- Local regression: a new approach for measurement system comparison analysis
- Accuracy in clinical chemistry – who will kiss Sleeping Beauty awake?
- A practical approach to instrument selection, evaluation, basic financial management and implementation in pathology and research
- Genetics and Molecular Diagnostics
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- Preliminary validation of real-time PCR assays for the identification of Yersinia pestis
- Biochemical and molecular investigation of two Korean patients with glycogen storage disease type III
- Are microarrays useful in the screening of ABCA4 mutations in Italian patients affected by macular degenerations?
- General Clinical Chemistry and Laboratory Medicine
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- Measurement of mycophenolic acid in plasma or serum by a commercial enzyme inhibition technique in comparison with a high performance liquid chromatography method
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