The effect of hypothyroidism, hyperthyroidism, and their treatment on parameters of oxidative stress and antioxidant status
-
Hüsamettin Erdamar
Abstract
Background: Free radical-mediated oxidative stress has been implicated in the etiopathogenesis of several autoimmune disorders. Also, there is growing evidence supporting the role of reactive oxygen species in the pathogenesis of thyroid disorders. The aim of this study was to investigate the influence of hypothyroidism, hyperthyroidism, and their treatments on the metabolic state of oxidative stress, and antioxidant status markers.
Methods: A total of 20 newly diagnosed patients with overt hypothyroidism due to Hashimoto's thyroiditis, 20 patients with overt hyperthyroidism due to Graves' disease, and 20 healthy subjects as the control group were enrolled in the study. Fasting blood samples (12 h), taken at the initiation, after the 30th and 60th day of therapy were analyzed for malondialdehyde, nitrite, vitamin E, vitamin A, β-carotene, ascorbate, and myeloperoxidase and superoxide dismutase activity. No patient presented additional risk factors for increased reactive oxygen species levels.
Results: Malondialdehyde, nitrite, vitamin E, and myeloperoxidase activity increased in patients with hypothyroidism. After 2 months, the levels of nitrite and vitamin E were reduced to control levels by treatment. The patients with hyperthyroidism had increased levels of malondialdehyde and myeloperoxidase activity in comparison with the controls. Treatment with propylthiouracil attenuated these increments after 1 month.
Conclusions: Our results reveal an increased generation of reactive oxygen species and impairment of the antioxidant system in patients with hyperthyroidism, and particularly in patients with hypothyroidism. These findings indicate that thyroid hormones have a strong impact on oxidative stress and the antioxidant system.
Clin Chem Lab Med 2008;46:1004–10.
©2008 by Walter de Gruyter Berlin New York
Articles in the same Issue
- Editorial
- CCLM: Evolving to meet the needs of today's laboratory professionals and scientists
- Bacterial detection and blood product contamination
- Editorial: Rapid diagnostic tests to detect pathogenic microorganisms
- Reviews
- Rapid methods for diagnosis of bloodstream infections
- Validation criteria for nucleic acid amplification techniques for bacterial infections
- Incidence of bacterial transmission and transfusion reactions by blood components
- Strategies of bacteria screening in cellular blood components
- Methods for the detection of bacterial contamination in blood products
- Bacteria detection by flow cytometry
- Rapid screening by real-time 16S rDNA PCR for bacterial contamination of blood products
- Microbial safety of cell based medicinal products – what can we learn from cellular blood components?
- Genetics and Molecular Diagnostics
- Screening for genetic heterogeneity in the interferon sensitivity determining region of the hepatitis C virus genome by polymerase chain reaction with melting curve analysis
- Thiopurine S-methyltransferase (TPMT) pharmacogenetics: three new mutations and haplotype analysis in the Estonian population
- Genetic testing for adult-type hypolactasia in Italian families
- Improved real-time detection of the H63D and S65C mutations associated with hereditary hemochromatosis using a SimpleProbe assay format
- Easy detection of 5,10-methylenetetrahydrofolate reductase 1298A/C genotype by mutagenically separated PCR assay
- General Clinical Chemistry and Laboratory Medicine
- Mediterranean diet and plasma concentration of inflammatory markers in old and very old subjects in the ZINCAGE population study
- Plasma interleukin-1β, -6, -8 and tumor necrosis factor-α as highly informative markers of pelvic inflammatory disease
- The effect of hypothyroidism, hyperthyroidism, and their treatment on parameters of oxidative stress and antioxidant status
- Breath isoprene – aspects of normal physiology related to age, gender and cholesterol profile as determined in a proton transfer reaction mass spectrometry study
- Prohormone brain natriuretic peptide (proBNP), BNP and N-terminal-proBNP circulating levels in chronic hemodialysis patients. Correlation with ventricular function, fluid removal and effect of hemodiafiltration
- A comparison of two different assays for determining S-100B in serum and urine
- Diagnostic, clinical and laboratory turnaround times in troponin T testing
- Validation and Outcome Studies
- An international comparability study to determine the sources of uncertainty associated with a non-competitive sandwich fluorescent ELISA
- A new general regression-based approach for method comparison studies
- Letters to the Editor
- Procalcitonin values in preeclamptic women are related to severity of disease
- Cross-reactivity between λ antiserum and fibrinogen: a double trap in interpretation of serum protein electrophoresis
- Erroneous result of white blood cell differential count in a patient with mixed hyperlipidemia
- Lack of association of GH T1663A variation and IGFBP3–202A/C polymorphism with risk of ischemic stroke in Chinese
- Erratum
- Quality planning and analytical quality requirements derived from biology
Articles in the same Issue
- Editorial
- CCLM: Evolving to meet the needs of today's laboratory professionals and scientists
- Bacterial detection and blood product contamination
- Editorial: Rapid diagnostic tests to detect pathogenic microorganisms
- Reviews
- Rapid methods for diagnosis of bloodstream infections
- Validation criteria for nucleic acid amplification techniques for bacterial infections
- Incidence of bacterial transmission and transfusion reactions by blood components
- Strategies of bacteria screening in cellular blood components
- Methods for the detection of bacterial contamination in blood products
- Bacteria detection by flow cytometry
- Rapid screening by real-time 16S rDNA PCR for bacterial contamination of blood products
- Microbial safety of cell based medicinal products – what can we learn from cellular blood components?
- Genetics and Molecular Diagnostics
- Screening for genetic heterogeneity in the interferon sensitivity determining region of the hepatitis C virus genome by polymerase chain reaction with melting curve analysis
- Thiopurine S-methyltransferase (TPMT) pharmacogenetics: three new mutations and haplotype analysis in the Estonian population
- Genetic testing for adult-type hypolactasia in Italian families
- Improved real-time detection of the H63D and S65C mutations associated with hereditary hemochromatosis using a SimpleProbe assay format
- Easy detection of 5,10-methylenetetrahydrofolate reductase 1298A/C genotype by mutagenically separated PCR assay
- General Clinical Chemistry and Laboratory Medicine
- Mediterranean diet and plasma concentration of inflammatory markers in old and very old subjects in the ZINCAGE population study
- Plasma interleukin-1β, -6, -8 and tumor necrosis factor-α as highly informative markers of pelvic inflammatory disease
- The effect of hypothyroidism, hyperthyroidism, and their treatment on parameters of oxidative stress and antioxidant status
- Breath isoprene – aspects of normal physiology related to age, gender and cholesterol profile as determined in a proton transfer reaction mass spectrometry study
- Prohormone brain natriuretic peptide (proBNP), BNP and N-terminal-proBNP circulating levels in chronic hemodialysis patients. Correlation with ventricular function, fluid removal and effect of hemodiafiltration
- A comparison of two different assays for determining S-100B in serum and urine
- Diagnostic, clinical and laboratory turnaround times in troponin T testing
- Validation and Outcome Studies
- An international comparability study to determine the sources of uncertainty associated with a non-competitive sandwich fluorescent ELISA
- A new general regression-based approach for method comparison studies
- Letters to the Editor
- Procalcitonin values in preeclamptic women are related to severity of disease
- Cross-reactivity between λ antiserum and fibrinogen: a double trap in interpretation of serum protein electrophoresis
- Erroneous result of white blood cell differential count in a patient with mixed hyperlipidemia
- Lack of association of GH T1663A variation and IGFBP3–202A/C polymorphism with risk of ischemic stroke in Chinese
- Erratum
- Quality planning and analytical quality requirements derived from biology
