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Breath isoprene – aspects of normal physiology related to age, gender and cholesterol profile as determined in a proton transfer reaction mass spectrometry study
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Ievgeniia Kushch
Veröffentlicht/Copyright:
22. Mai 2008
Abstract
Background: This study was performed to clarify variations in breath isoprene concentrations with age, gender, body mass index (BMI) and total serum cholesterol. Our cohort consisted of 205 adult volunteers of different smoking background without health complaints. Total cholesterol in blood serum was measured in 79 of these volunteers.
Methods: Mixed expiratory exhaled breath was sampled using Tedlar bags. Concentrations of isoprene were then determined using proton transfer reaction-mass spectrometry.
Results: Isoprene concentrations ranged from 5.8 to 274.9 ppb, with an overall geometric mean (GM) of 99.3 ppb. There was no statistically significant difference in mean isoprene in breath between males and females (GM 105.4 and 95.5 ppb, respectively). Ageing led to a decrease in concentration in men, with an estimated slope of the regression line for log-transformed isoprene concentrations of –0.0049, but did not influence isoprene levels in women. We did not observe any significant correlation between isoprene breath content and cholesterol level in blood, even after adjusting for the possible influence of age. Similarly, no correlation was found between isoprene levels and BMI.
Conclusions: Isoprene concentrations in exhaled breath showed gender-specific correlations with respect to age. Further investigations are necessary to clarify the relation between isoprene concentrations in exhaled breath and cholesterol levels and synthesis rates in blood.
Clin Chem Lab Med 2008;46:1011–8.
Keywords: age; body mass index; breath analysis; gender; isoprene; proton transfer reaction-mass spectrometry (PTR-MS); serum total cholesterol
Received: 2007-12-13
Accepted: 2008-2-26
Published Online: 2008-05-22
Published in Print: 2008-07-01
©2008 by Walter de Gruyter Berlin New York
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Schlagwörter für diesen Artikel
age;
body mass index;
breath analysis;
gender;
isoprene;
proton transfer reaction-mass spectrometry (PTR-MS);
serum total cholesterol
Artikel in diesem Heft
- Editorial
- CCLM: Evolving to meet the needs of today's laboratory professionals and scientists
- Bacterial detection and blood product contamination
- Editorial: Rapid diagnostic tests to detect pathogenic microorganisms
- Reviews
- Rapid methods for diagnosis of bloodstream infections
- Validation criteria for nucleic acid amplification techniques for bacterial infections
- Incidence of bacterial transmission and transfusion reactions by blood components
- Strategies of bacteria screening in cellular blood components
- Methods for the detection of bacterial contamination in blood products
- Bacteria detection by flow cytometry
- Rapid screening by real-time 16S rDNA PCR for bacterial contamination of blood products
- Microbial safety of cell based medicinal products – what can we learn from cellular blood components?
- Genetics and Molecular Diagnostics
- Screening for genetic heterogeneity in the interferon sensitivity determining region of the hepatitis C virus genome by polymerase chain reaction with melting curve analysis
- Thiopurine S-methyltransferase (TPMT) pharmacogenetics: three new mutations and haplotype analysis in the Estonian population
- Genetic testing for adult-type hypolactasia in Italian families
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