Artikel
Lizenziert
Nicht lizenziert
Erfordert eine Authentifizierung
Limits of preservation of samples for urine strip tests and particle counting
-
Timo Kouri
Veröffentlicht/Copyright:
10. März 2008
Abstract
Background: Preservation of urine samples is important for centralised laboratory services with automated instruments.
Methods: A multicentre evaluation was carried out to assess preservative tubes from BD Diagnostics-Pre-analytical Systems and from Greiner Bio-One for test strip reading (documented at the level of remission values), for particle counting by flow cytometers (UF-100) and for visual microscopy. Failures were expressed as percentages of originally positive samples beyond a two-fold change (+100% or –50%) from the original values.
Results: The preservative-containing BD Plus C&S plastic, BD Plus UAP and Greiner Stabilur tubes succeeded in preservation of test strip results for 6–24 h (exceptions were glucose and nitrite tests). Greiner boric acid tube showed false negative results in leukocyte, protein and ketone strip tests immediately after adding the preservative. Urine red blood cell counts (with Sysmex UF-100) were preserved for 5 h in BD Plus C&S plastic and Greiner Stabilur tubes (Greiner tubes having clearly larger preservative-related background). Bacteria or white blood cell counting succeeded in BD Plus C&S plastic tubes for 5 or 24 h, respectively, but up to 72 h in Greiner Stabilur tubes. In visual microscopy, the Greiner Stabilur tube was slightly better than the BD Plus C&S plastic tube.
Conclusions: Urine specimens can be transported at +20°C on the day of collection if preserved properly. Longer delays need careful planning with current preservatives. Flow cytometry with UF-100 is sensitive to non-dissolved preservative remnants.
Clin Chem Lab Med 2008;46:703–13.
Received: 2007-10-30
Accepted: 2008-1-8
Published Online: 2008-03-10
Published in Print: 2008-05-01
©2008 by Walter de Gruyter Berlin New York
Sie haben derzeit keinen Zugang zu diesem Inhalt.
Sie haben derzeit keinen Zugang zu diesem Inhalt.
Artikel in diesem Heft
- Editorial
- Comparability of tumor marker immunoassays: still an important issue for clinical diagnostics?
- Review
- The diagnostic role of autoantibodies in the prediction of organ-specific autoimmune diseases
- Validation and Outcome Studies
- Alternative antibody for the detection of CA125 antigen: a European multicenter study for the evaluation of the analytical and clinical performance of the Access® OV Monitor assay on the UniCel® DxI 800 Immunoassay System
- Alternative antibody for the detection of CA19-9 antigen: a European multicenter study for the evaluation of the analytical and clinical performance of the Access® GI Monitor assay on the UniCel® DxI 800 Immunoassay System
- Alternative antibody for the detection of CA15-3 antigen: a European multicenter study for the evaluation of the analytical and clinical performance of the Access® BR Monitor assay on the UniCel® DxI 800 Immunoassay System
- Toward metrological traceability in the determination of prostate-specific antigen (PSA): calibrating Beckman Coulter Hybritech Access PSA assays to WHO standards compared with the traditional Hybritech standards
- Multicentre evaluation of a new point-of-care test for the determination of CK-MB in whole blood
- Down's syndrome screening: population statistic dependency of screening performance
- General Clinical Chemistry and Laboratory Medicine
- Association between plasma thiols and immune activation marker neopterin in stable coronary heart disease
- Role for mitochondrial uncoupling protein-2 (UCP2) in hyperhomocysteinemia and venous thrombosis risk?
- Plasma procalcitonin measured by time-resolved amplified cryptate emission (TRACE) in liver transplant patients. A prognosis marker of early infectious and non-infectious postoperative complications
- Interactions of lipoprotein(a) with diabetes mellitus, apolipoprotein B and cholesterol enhance the prognostic values for coronary artery disease
- Association of thyroid stimulating hormone and coronary lipid risk factors with lipid peroxidation in hypothyroidism
- The effect of the mode of delivery on the maternal-neonatal carnitine blood levels and antioxidant status
- Time to reconsider the clinical value of immunoglobulin G4 to foods?
- Reference Values
- Determination of reference intervals for 26 commonly measured biochemical analytes with consideration of long-term within-individual variation
- Beverage-specific effects of ethanol consumption on its biological markers
- Limits of preservation of samples for urine strip tests and particle counting
- Reference ranges and diagnostic thresholds of laboratory markers of cardiac damage and dysfunction in a population of apparently healthy black Africans
- Letters to the Editor
- External quality assessment schemes for real-time PCR: a statistical procedure to corrective actions
- Application of two different microarray-based copy-number detection methodologies – array-comparative genomic hybridization and array-multiplex amplifiable probe hybridization – with identical amplifiable target sequences
- Criticism to the article: “Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: I. Analyte definition and proposal of a candidate reference method.” Authors: J.O. Jeppsson et al. Clin Chem Lab Med 2007;45(4):558–562
- Standardization of carbohydrate-deficient transferrin: reply to the letter by Tagliaro and Bortolotti
- Comment on: Bellini C, Serra G, Risso D, Mazzella M, Bonioli E. Reliability assessment of glucose measurement by HemoCue analyser in a neonatal intensive care unit. Clin Chem Lab Med 2007;45(11):1549–54
- Reliability assessment of glucose measurement by HemoCue analyzer in a neonatal intensive care unit: reply to Dr. Joakim Hagvik
Schlagwörter für diesen Artikel
automation;
particle counting;
preservation;
test strips;
urinalysis
Artikel in diesem Heft
- Editorial
- Comparability of tumor marker immunoassays: still an important issue for clinical diagnostics?
- Review
- The diagnostic role of autoantibodies in the prediction of organ-specific autoimmune diseases
- Validation and Outcome Studies
- Alternative antibody for the detection of CA125 antigen: a European multicenter study for the evaluation of the analytical and clinical performance of the Access® OV Monitor assay on the UniCel® DxI 800 Immunoassay System
- Alternative antibody for the detection of CA19-9 antigen: a European multicenter study for the evaluation of the analytical and clinical performance of the Access® GI Monitor assay on the UniCel® DxI 800 Immunoassay System
- Alternative antibody for the detection of CA15-3 antigen: a European multicenter study for the evaluation of the analytical and clinical performance of the Access® BR Monitor assay on the UniCel® DxI 800 Immunoassay System
- Toward metrological traceability in the determination of prostate-specific antigen (PSA): calibrating Beckman Coulter Hybritech Access PSA assays to WHO standards compared with the traditional Hybritech standards
- Multicentre evaluation of a new point-of-care test for the determination of CK-MB in whole blood
- Down's syndrome screening: population statistic dependency of screening performance
- General Clinical Chemistry and Laboratory Medicine
- Association between plasma thiols and immune activation marker neopterin in stable coronary heart disease
- Role for mitochondrial uncoupling protein-2 (UCP2) in hyperhomocysteinemia and venous thrombosis risk?
- Plasma procalcitonin measured by time-resolved amplified cryptate emission (TRACE) in liver transplant patients. A prognosis marker of early infectious and non-infectious postoperative complications
- Interactions of lipoprotein(a) with diabetes mellitus, apolipoprotein B and cholesterol enhance the prognostic values for coronary artery disease
- Association of thyroid stimulating hormone and coronary lipid risk factors with lipid peroxidation in hypothyroidism
- The effect of the mode of delivery on the maternal-neonatal carnitine blood levels and antioxidant status
- Time to reconsider the clinical value of immunoglobulin G4 to foods?
- Reference Values
- Determination of reference intervals for 26 commonly measured biochemical analytes with consideration of long-term within-individual variation
- Beverage-specific effects of ethanol consumption on its biological markers
- Limits of preservation of samples for urine strip tests and particle counting
- Reference ranges and diagnostic thresholds of laboratory markers of cardiac damage and dysfunction in a population of apparently healthy black Africans
- Letters to the Editor
- External quality assessment schemes for real-time PCR: a statistical procedure to corrective actions
- Application of two different microarray-based copy-number detection methodologies – array-comparative genomic hybridization and array-multiplex amplifiable probe hybridization – with identical amplifiable target sequences
- Criticism to the article: “Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: I. Analyte definition and proposal of a candidate reference method.” Authors: J.O. Jeppsson et al. Clin Chem Lab Med 2007;45(4):558–562
- Standardization of carbohydrate-deficient transferrin: reply to the letter by Tagliaro and Bortolotti
- Comment on: Bellini C, Serra G, Risso D, Mazzella M, Bonioli E. Reliability assessment of glucose measurement by HemoCue analyser in a neonatal intensive care unit. Clin Chem Lab Med 2007;45(11):1549–54
- Reliability assessment of glucose measurement by HemoCue analyzer in a neonatal intensive care unit: reply to Dr. Joakim Hagvik
