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5′ ins/del and 3′ VNTR polymorphisms in the apolipoprotein B gene in relation to lipids and coronary artery disease
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Lamia Rebhi
Published/Copyright:
February 6, 2008
Abstract
Background: Studies that considered apolipoprotein B (APOB) gene polymorphisms as risk factors for coronary artery disease (CAD) have reported conflicting results. We sought to analyze the association between 5′ ins/del and 3′ VNTR polymorphisms of APOB, lipid parameters and CAD risk.
Methods: We recruited 251 patients with CAD, documented by coronary angiography, and 94 controls. Genotyping was performed by PCR. Lipids and apolipoproteins were measured.
Results: 5′ ins/del (ins/ins, ins/del, del/del) and 3′ VNTR (LL, SS, LS) polymorphism frequencies were significantly (p<0.05) different between controls and CAD patients. LL and del/del were significantly associated with higher levels of apolipoprotein B (apoB), total cholesterol/high-density lipoprotein cholesterol ratio and apoB/apoA-I ratio (p<0.05) and with increased risk of CAD. The odds ratio for significant coronary stenosis associated with del/del was 3.2 (95% CI 1.6–36.42) (p=0.032) and with LL was 2.2 (95% CI 1.1–5.1) (p=0.042).
Conclusions: The two polymorphisms exert an impact on lipid levels and contribute to the susceptibility to the development of CAD.
Clin Chem Lab Med 2008;46:329–34.
Keywords: apolipoprotein B; coronary artery disease; genotypes; 5′ ins/del; lipids; polymorphism; 3′ VNTR
Received: 2007-8-30
Accepted: 2007-11-15
Published Online: 2008-02-6
Published in Print: 2008-03-01
©2008 by Walter de Gruyter Berlin New York
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Keywords for this article
apolipoprotein B;
coronary artery disease;
genotypes;
5′ ins/del;
lipids;
polymorphism;
3′ VNTR
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