The effects of adrenocorticotrophic hormone and cortisol on homocysteine and vitamin B concentrations
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Anna L. Berg
Abstract
Background: Homocysteine metabolism is mainly governed by serum concentrations of folate and vitamin B12, renal function and genetic factors. It is also well documented that endocrinological disturbances influence homocysteine metabolism. However, studies on the hormones of the hypothalamic-pituitary-adrenal axis have given conflicting results.
Methods: A total of 30 healthy young males were randomised into three groups of equal size; one group received adrenocorticotrophic hormone (ACTH)1–24 1mg i.m. daily for 4days, another group was treated with cortisol 50mg i.m. t.i.d. for 4days, while a control group was observed for 4days. Fasting blood samples were collected before and after treatment or observation.
Results: The pattern of changes was the same for the ACTH and cortisol groups; there were significant decreases in serum concentrations of folate (23% and 24%) and cobalamines (13% and 19%) and decreases in plasma total homocysteine concentrations that did not reach significance. There were no changes in the control group.
Conclusions: The virtually identical pattern of changes in both treatment groups suggests that the effects were mediated by cortisol. The reductions in serum concentrations of folate and cobalamines call for further research.
References
1. Ford ES, Smith SJ, Stroup DF, Steinberg KK, Mueller PW, Thacker SB. Homocyst(e)ine and cardiovascular disease: a systematic review of the evidence with special emphasis on case-control studies and nested case-control studies. Int J Epidemiol 2002; 31:59–70.10.1093/ije/31.1.59Search in Google Scholar
2. The Homocysteine Studies Collaboration. Homocysteine and risk of ischemic heart disease and stroke: a meta-analysis. J Am Med Assoc 2002;288:2015–22.10.1001/jama.288.16.2015Search in Google Scholar
3. Welch GN, Loscalzo J. Homocysteine and atherothrombosis. N Engl J Med 1998; 338:1042–50.10.1056/NEJM199804093381507Search in Google Scholar
4. Nedrebo BG, Ericsson UB, Nygard O, Refsum H, Ueland PM, Aakvaag A, et al. Plasma total homocysteine levels in hyperthyroid and hypothyroid patients. Metabolism 1998; 47:89–3.10.1016/S0026-0495(98)90198-6Search in Google Scholar
5. Smolders RG, van der Mooren MJ, Sipkema P, Kenemans P. Estrogens, homocysteine, vasodilation and menopause: basic mechanisms, interactions and clinical implications. Gynecol Endocrinol 2003; 17:339–54.Search in Google Scholar
6. Martinez-Taboada VM, Bartolome MJ, Fernandez-Gonzalez MD, Blanco R, Rodrigues-Valverde V, Lopesz-Hoyos M. Homocysteine levels in polymyalgia rheumatica and giant cell arteritis: influence of corticosteroid therapy. Rheumatology 2003; 42:1055–61.10.1093/rheumatology/keg293Search in Google Scholar
7. Terzolo M, Allasino B, Bosio S, Brusa E, Daffara F, Ventura M. Hyperhomocysteinemia in patients with Cushing's syndrome. J Clin Endocrinol Metab 2004; 89:3745–51.10.1210/jc.2004-0079Search in Google Scholar
8. Faggiano A, Melis D, Alfieri R, De Martino MC, Filippella M, Milone F, et al. Sulfur amino acids in Cushing's disease: insight in homocysteine and taurine levels in patients with active and cured disease. J Clin Endocrinol Metab 2005; 90:6616–22.10.1210/jc.2005-0656Search in Google Scholar
9. Lazzerini PE, Capecchi PL, Bisogno S, Galeazzi M, Marcolongo R, Pasini FL. Reduction in plasma homocysteine levels in patients with rheumatoid arthritis given pulsed glucocorticoid treatment. Ann Rheum Dis 2003; 62:694–5.10.1136/ard.62.7.694Search in Google Scholar
10. Kim MH, Kim E, Passen EL, Meyer J, Kang SS. Cortisol and estradiol: nongenetic factors for hyperhomocyst(e)inemia. Metabolism 1997; 46:247–9.10.1016/S0026-0495(97)90248-1Search in Google Scholar
11. Dierkes J, Westphal S, Luley C. The effect of fibrates and other lipid-lowering drugs on plasma homocysteine levels. Expert Opin Drug Saf 2004; 3:101–11.10.1517/14740338.3.2.101Search in Google Scholar
12. Berg AL, Nilsson-Ehle P. Direct effect on corticotropin plasma lipoprotein metabolism in man – studies in vivo and in vitro. Metabolism 1994; 43:90–7.10.1016/0026-0495(94)90162-7Search in Google Scholar
13. Rafnsson AT, Johannsson M, Olafsson I, Dallongeville J, Erfurth EM, Berg AL, et al. Effects of different doses of adrenocorticotrophic hormone on the serum lipoprotein profile in healthy subjects. Basic Clin Pharmacol Toxicol 2005; 97:86–90.10.1111/j.1742-7843.2005.pto_108.xSearch in Google Scholar
14. Andersson A, Isaksson A, Brattström L, Hultberg B. Homocysteine and other thiols determined in plasma by HPLC and thiol-specific postcolumn derivatization. Clin Chem 1993; 39:1590–97.10.1093/clinchem/39.8.1590Search in Google Scholar
15. Refsum H, Guttormsen AB, Fiskerstrand T, Ueland PM. On the formation and fate of total plasma homocysteine. In: Graham I, Refsum H, Rosenberg IH, Ueland PM, editors. Homocysteine metabolism: from basic science to clinical medicine. Boston, MA: Kluwer Academic, 1997:23–30.Search in Google Scholar
16. Finkelstein JD, Kyle WE, Harris BJ. Methionine metabolism in mammals. Regulation of homocysteine methyltransferases in rat tissue. Arch Biochem Biophys 1971; 146:84–92.10.1016/S0003-9861(71)80044-9Search in Google Scholar
©2006 by Walter de Gruyter Berlin New York
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