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Comparative evaluation of a new immunoradiometric assay for corticotropin
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Charles W. Wilkinson
Published/Copyright:
September 21, 2011
Abstract
We have characterized the performance of a commercial two-site immunoradiometric assay for manual in vitro diagnostic measurement of plasma corticotropin from Scantibodies Laboratory. We compared the results with those of a similar commonly used assay from Nichols Institute Diagnostics that has recently been withdrawn from production. The lower detection limit, range of the standard curve, cross-reactivity, and intra-assay and inter-assay imprecision of the two assays are very similar. Measurement of clinical samples and a series of samples from an experimental subject demonstrate high correlations between the two assays. These factors, together with recent clearance by the United States Food and Drug Administration for manual in vitro diagnostic measurement, make the Scantibodies corticotropin immunoradiometric assay an appropriate replacement for the Nichols assay.
Keywords: corticotropin; diagnostic techniques and procedures; immunoradiometric assay (IRMA); sensitivity and specificity
References
1. Raff H, Findling JW. Evaluation of a new immunoradiometric (IRMA) assay for ACTH in normal subjects and patients with Cushing's syndrome. Clin Chem 1989; 35:596–600.10.1093/clinchem/35.4.596Search in Google Scholar
2. Findling JW, Kehoe ME, Shaker JL, Raff H. Routine inferior petrosal sinus sampling in the differential diagnosis of ACTH-dependent Cushing's syndrome: early recognition of the occult ectopic ACTH syndrome. J Clin Endocrinol Metab 1991; 73:408–13.10.1210/jcem-73-2-408Search in Google Scholar PubMed
Received: 2006-1-3
Accepted: 2006-2-20
Published Online: 2011-9-21
Published in Print: 2006-5-1
©2006 by Walter de Gruyter Berlin New York
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Keywords for this article
corticotropin;
diagnostic techniques and procedures;
immunoradiometric assay (IRMA);
sensitivity and specificity
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