A longitudinal evaluation of urinary glycosaminoglycan excretion in normoalbuminuric type 1 diabetic patients
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Pierina De Muro
Abstract
Background: Previously, we found high urinary glycosaminoglycan (GAG) concentration, together with an altered electrophoretic pattern, in normoalbuminuric type 1 diabetic subjects with hemoglobin A1c (HbA1c) ≥8.0%. The purpose of this study was long-term evaluation of GAG excretion variations in these patients compared to those with HbA1c <8.0% at baseline who maintained better metabolic control over time.
Methods: We enrolled 26 normotensive, normoalbuminuric type 1 diabetic patients and divided them into two groups according to mean HbA1c levels during the follow-up period. GAGs were isolated from 24-h urine samples on two separate occasions, at baseline and after a mean (±SD) follow-up of 6.8±1.1years.
Results: All patients remained normoalbuminuric at follow-up, and had normal urinary α1-microglobulin levels. In patients with HbA1c <8.0%, total GAG levels and low sulfated chondroitin sulfate-proteoglycan/chondroitin sulfate ratio were almost unchanged during the follow-up period. In contrast, these increased in patients with HbA1c≥8.0% and were significantly related to both HbA1c levels and the duration of poor glycemic control.
Conclusions: Our results show a strong influence of hyperglycemic environment on GAG metabolism in diabetes and indicate that the distribution pattern of urinary GAGs, besides their total concentration, may be predictive of altered GAG metabolism in type 1 diabetes.
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©2006 by Walter de Gruyter Berlin New York
Articles in the same Issue
- CCLM: Expanding the science worldwide
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- Immunochemical quantification of free immunoglobulin light chains from an analytical perspective
- De novo deletion removes a conserved motif in the C-terminus of ABCA4 and results in cone-rod dystrophy
- Molecular detection of squamous cell carcinoma antigen transcripts in peripheral blood of cancer patients
- Influence of human haptoglobin polymorphism on oxidative stress induced by free hemoglobin on red blood cells
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- Association of high-sensitive C-reactive protein with advanced stage β-cell dysfunction and insulin resistance in patients with type 2 diabetes mellitus
- A longitudinal evaluation of urinary glycosaminoglycan excretion in normoalbuminuric type 1 diabetic patients
- National survey on the execution of the oral glucose tolerance test (OGTT) in a representative cohort of Italian laboratories
- The reduction of cholesteryl linoleate in lipoproteins: an index of clinical severity in β-thalassemia/Hb E
- Alterations in serum glycosaminoglycan profiles in Graves' patients
- Alterations in anti-oxidative defence enzymes in erythrocytes from sporadic amyotrophic lateral sclerosis (SALS) and familial ALS patients
- Sandwich ELISAs for soluble immunoglobulin superfamily receptor translocation-associated 2 (IRTA2)/FcRH5 (CD307) proteins in human sera
- Utilizing ultrafiltration to remove alkaline phosphatase from clinical analyzer water
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- Insufficient filling of vacuum tubes as a cause of microhemolysis and elevated serum lactate dehydrogenase levels. Use of a data-mining technique in evaluation of questionable laboratory test results
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Articles in the same Issue
- CCLM: Expanding the science worldwide
- Factor V Leiden, prothrombin G20210A substitution and hormone therapy: indications for molecular screening
- Immunochemical quantification of free immunoglobulin light chains from an analytical perspective
- De novo deletion removes a conserved motif in the C-terminus of ABCA4 and results in cone-rod dystrophy
- Molecular detection of squamous cell carcinoma antigen transcripts in peripheral blood of cancer patients
- Influence of human haptoglobin polymorphism on oxidative stress induced by free hemoglobin on red blood cells
- Real-time RT-PCR quantification of PRAME gene expression for monitoring minimal residual disease in acute myeloblastic leukaemia
- Association of high-sensitive C-reactive protein with advanced stage β-cell dysfunction and insulin resistance in patients with type 2 diabetes mellitus
- A longitudinal evaluation of urinary glycosaminoglycan excretion in normoalbuminuric type 1 diabetic patients
- National survey on the execution of the oral glucose tolerance test (OGTT) in a representative cohort of Italian laboratories
- The reduction of cholesteryl linoleate in lipoproteins: an index of clinical severity in β-thalassemia/Hb E
- Alterations in serum glycosaminoglycan profiles in Graves' patients
- Alterations in anti-oxidative defence enzymes in erythrocytes from sporadic amyotrophic lateral sclerosis (SALS) and familial ALS patients
- Sandwich ELISAs for soluble immunoglobulin superfamily receptor translocation-associated 2 (IRTA2)/FcRH5 (CD307) proteins in human sera
- Utilizing ultrafiltration to remove alkaline phosphatase from clinical analyzer water
- Measurement of serum monoclonal components: comparison between densitometry and capillary zone electrophoresis
- Salivary aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase: possible markers in periodontal diseases?
- Reticulocyte count, mean reticulocyte volume, immature reticulocyte fraction, and mean sphered cell volume in elite athletes: reference values and comparison with the general population
- Serum homocysteine levels and paraoxonase 1 activity in preschool aged children in Greece
- The effects of adrenocorticotrophic hormone and cortisol on homocysteine and vitamin B concentrations
- Plasma, salivary and urinary cotinine in non-smoker Italian women exposed and unexposed to environmental tobacco smoking (SEASD study)
- Cut-off values for total serum immunoglobulin E between non-atopic and atopic children in north-west Croatia
- Thyroglobulin assay during thyroxine treatment in low-risk differentiated thyroid cancer management: comparison with recombinant human thyrotropin-stimulated assay and imaging procedures
- Evaluation of serum levels of p53 in hepatocellular carcinoma in Egypt
- Insufficient filling of vacuum tubes as a cause of microhemolysis and elevated serum lactate dehydrogenase levels. Use of a data-mining technique in evaluation of questionable laboratory test results
- Evaluation of three different specimen types (serum, plasma lithium heparin and serum gel separator) for analysis of certain analytes: clinical significance of differences in results and efficiency in use
- Comparative evaluation of a new immunoradiometric assay for corticotropin
- Mast cells in atherosclerosis as a source of the cytokine RANKL
- Falsely increased total serum protein due to dextran interference
