Evaluation of serum levels of p53 in hepatocellular carcinoma in Egypt
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Mohamed A. El Far
Abstract
Hepatocellular carcinoma (HCC) is one of the most common causes of cancer-induced death. Somatic mutation of the p53 gene is the most common genetic abnormality so far described in human cancer and there is evidence that supports a high level of p53 alterations in HCC. The aim of this study was to investigate serum levels of p53 in Egyptian patients with HCC, and its relation to other prognostic factors such as tumor grade, α-fetoprotein (AFP), and liver function tests in an attempt to clarify their significance in the pathogenesis of the disease. Liver function tests were carried out and AFP and p53 levels were measured for all individuals studied. Our results show that detection of p53 increased the frequency of HCC prediction from 79.5% to 86.3%. Moreover, significant positive correlation between p53 and tumor size (cm) for tumor grade II and III was identified. In conclusion, serum concentration of p53 protein may be a convenient and useful non-invasive screening test for prediction of HCC.
References
1. Vauthey JN. Hepatobiliary cancer. Cancer Control 1998; 5:32–3.10.1177/107327489800503S12Search in Google Scholar
2. Oka H, Kurioka N, Kim K, Kanno T, Kuroki K, Mizguchi Y, et al. Prospective study of early detection of hepatocellular carcinoma in patients with cirrhosis. Hepatology 1990; 12:680–7.10.1002/hep.1840120411Search in Google Scholar
3. London TW. Hepatocellular carcinoma. Etiology and pathology. In: Proceedings of the ASCO 34th annual meeting, Los Angeles, CA, May 1998:168–75.Search in Google Scholar
4. Hsu YL, Kuo PL, Chiang LC, Lin CC. Involvement of p53, nuclear factor κB and Fas/Fas ligand in induction of apoptosis and cell cycle arrest by saikosaponin d in human hepatoma cell lines. Cancer Lett 2004; 213:213–21.10.1016/j.canlet.2004.03.044Search in Google Scholar
5. Volkmann M, Hofmann WJ, Muller M, Karth U, Otto G, Zentgraf H, et al. p53 over-expression is frequent in Europeans in hepatocellular carcinoma and largely independent of the codon 249 hot spot mutation. Oncogene 1994; 9:195–204.Search in Google Scholar
6. Lui WY, Chiu ST, Chiu JH, Loong CC, Chau GY, King KL, et al. Evaluation of a simplified staging system for prognosis of hepatocellular carcinoma. J Formos Med Assoc 1999; 98:248–53.Search in Google Scholar
7. Peng SY, Chen WJ, Lai PL, Jeng YMS. High alpha-fetoprotein level correlates with high stage, early recurrence and poor prognosis of hepatocellular carcinoma: significance of hepatitis virus infection, age, p53 and beta-catenin mutations. Int J Cancer 2004; 112:44–50.10.1002/ijc.20279Search in Google Scholar
8. Kannangai R, Wang J, Liu QZ, Sahin F, Torbenson M. Survivin overexpression in hepatocellular carcinoma is associated with p53 dysregulation. Int J Gastrointest Cancer 2005; 35:53–60.10.1385/IJGC:35:1:053Search in Google Scholar
9. Reeves ME, De Matteo RB. Genes and viruses in hepatobiliary neoplasia. Semin Surg Oncol 2000; 19:84–93.10.1002/1098-2388(200009)19:2<84::AID-SSU2>3.0.CO;2-0Search in Google Scholar
10. Okada T, Iizuka N, Yamada-Okabe H, Mori N, Tamesa T, Takemoto N, et al. Gene expression profile linked to p53 status in hepatitis C virus-related hepatocellular carcinoma. FEBS Lett 2003; 555:583–90.10.1016/S0014-5793(03)01345-0Search in Google Scholar
11. Cho JW, Park K, Kweon GR, Park JC, Lee JC, Baek WK, et al. Modulation of cell death sensitivity by mutant p53 in HCV core-expressing cells. Int J Mol Med 2005; 15:475–80.10.3892/ijmm.15.3.475Search in Google Scholar
12. Di Bisceglie AM. Hepatitis C and hepatocellular carcinoma. Hepatology 1997; 26(Suppl 1):345–85.10.1002/hep.510260706Search in Google Scholar
13. Lin J, Zhu MH, Qu JH, Li FM, Ni CR. Effects of hepatitis B virus X gene on p21(WAF1) expression through p53-dependent and p53-independent pathways. Ai Zheng 2004; 23:749–55.Search in Google Scholar
14. Okada S, Sato T, Okusaka T, Ikeda M, Nakasuka H, Kosakamoto H, et al. Past exposure to hepatitis B virus as a risk factor for hepatocellular carcinoma in patients with chronic liver disease. Br J Cancer 1998; 77:2028–31.10.1038/bjc.1998.337Search in Google Scholar
15. Shieh YS, Nguyen C, Vocal MV, Chu H. Tumor suppressor p53 gene in hepatitis C and B virus associated human HCC. Int J Cancer 1993; 54:558–62.10.1002/ijc.2910540407Search in Google Scholar PubMed
16. Ray RB, Steele R, Meyer K, Ray R. Transcription repression of p53 promoter by hepatitis C virus core protein. J Biol Chem 1997; 272:10983–6.10.1074/jbc.272.17.10983Search in Google Scholar PubMed
©2006 by Walter de Gruyter Berlin New York
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