The secretion of ibuprofen metabolites interferes with the capillary chromatography of urinary homovanillic acid and 4-hydroxy-3-methoxymandelic acid in neuroblastoma diagnosis
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Isabella Levreri
Abstract
Neuroblastoma is the most common extracranial solid tumor in children. Abnormal secretion of catechol-amines in tissues and body fluids allows for the differential diagnosis of neuroblastoma from other neoplasms and its distinction from non-neoplastic inflammatory diseases. This is achieved by assaying homovanillic acid and 4-hydroxy-3-methoxymandelic acid, the catabolites of catecholamine metabolism. In the course of an evaluation of children with suspected neuroblastoma, homovanillic acid and 4-hydroxy-3-methoxymandelic acid were analyzed in urine samples by capillary gas chromatography with flame ionization detection after extraction and derivatization of these compounds as trimethylsilyl derivatives. In three urine samples a significant increase in biogenic amines was observed, but these results were not confirmed by thin-layer chromatography. Patient history revealed that these children had been treated with ibuprofen, an analgesic and anti-inflammatory drug. To verify how ibuprofen or its metabolites may have interfered with capillary gas chromatography with flame ionization detection, we analyzed the same samples by capillary gas chromatography-mass spectrometry. In urine samples from patients on the drug, the presence of a peak identified as the trimethylsilyl ester of hydroxyibuprofen, which had the same retention time as 4-hydroxy-3-methoxymandelic acid, was found to interfere with the capillary gas chromatography with flame ionization detection analysis of the metabolite. This interference must be taken into account during the laboratory diagnosis of neuroblastoma.
References
1 De Bernardi B, Nicolas B, Boni L, Indolfi P, Carli M, Cordero Di Montezemolo L, Donfrancesco A, Pession A, Provenzi M, di Cataldo A, Rizzo A, Tonini GP, Dallorso S, Conte M, Gambini C, Garaventa A, Bonetti F, Zanazzo A, D'Angelo P, Bruzzi P; Italian Co-Operative Group for Neuroblastoma. Disseminated neuroblastoma in children older than one year at diagnosis: comparable results with three consecutive high-dose protocols adopted by the Italian Co-Operative Group for Neuroblastoma. J Clin Oncol 2003; 21: 1592–601. 10.1200/JCO.2003.05.191Search in Google Scholar
2 Jaffe N. Neuroblastoma review of the literature and an examination of its enigmatic character. Cancer Treat Rep 1971; 3: 61–82. 10.1016/S0305-7372(76)80005-9Search in Google Scholar
3 Hajes FA, Smith EI. Neuroblastoma. In: Pizzo PA, Poplack DG, editors. Principles and practice of pediatric oncology. Philadelphia: Lippincott Company, 1989:607–22. Search in Google Scholar
4 De Bernardi B. Neuroblastoma. Pediatr Med Chir 1984; 6: 229–34. Search in Google Scholar
5 Wadman SK, Ketting D, Voute PA. Gas chromatographic determination of urinary vanilglycolic acid, vanilglycol, vanilacetic acid and vanillactic acid chemical parameters for the diagnosis of neurogenic tumours and the evaluation of their treatment. Clin Chim Acta 1976; 72: 49–68. 10.1016/0009-8981(76)90036-XSearch in Google Scholar
6 Candito M, Thyss A, Albertini M, Deville A, Politano S, Mariani R, et al. Methylated catecholamine metabolites for diagnosis of neuroblastoma. Med Pediatr Oncol 1992; 20: 215–20. 10.1002/mpo.2950200306Search in Google Scholar
7 Bennet MJ, Sherwood WG, Bhala A, Hale DE. Identification of urinary metabolites of (±)-2-(p-isobutylphenyl)propionic acid (ibuprofen) by routine organic acid screening. Clin Chim Acta 1992; 210: 55–62. 10.1016/0009-8981(92)90045-RSearch in Google Scholar
8 Kepp DR, Sidelmann UG, Tjonerlund J, Hansen SH. Simultaneous quantitative determination of the major phase I and II metabolites of Ibuprofen in biological fluid by high-performance liquid chromatography on dynamically modified silica. J Chromatogr Biomed Sci Appl 1997; 696: 235–41. 10.1016/S0378-4347(97)00239-9Search in Google Scholar
9 Maurer HH, Tauvel FX, Kraemer T. Screening procedure for detection of non-steroidal anti-inflammatory drugs and their metabolites in urine as part of a systematic toxicological analysis procedure for acidic drugs and poisons by gas chromatography-mass spectrometry after extractive methylation. J Anal Toxicol 2001; 25: 237–44. 10.1093/jat/25.4.237Search in Google Scholar
10 Di Bussolo JM, Gant JR, Kerber D. Instrumental consideration in catecholamines analysis using liquid chroma-tography with electrochemical detection. Chromatogr Newslett 1983; 11-1: 27–9. Search in Google Scholar
11 Abeling NG, van Gennip AH, Overmars H, Voute PA. Simultaneous determination of catecholamines and metanephrines in urine by HPLC with fluorimetric detection. Clin Chim Acta 1984; 137: 211–26. 10.1016/0009-8981(84)90181-5Search in Google Scholar
12 Marchese N, Canini S, Fabi L, Famularo L. Paediatric reference values for urinary catecholamine metabolites evaluated by high performance liquid chromatography and electrochemical detection. Eur J Clin Chem Clin Biochem 1997; 35: 533–7. 10.1515/cclm.1997.35.7.533Search in Google Scholar PubMed
13 Marchese N, Caruso U. Quantitative measurement of 4-hydroxy-3-methoxymandelic and homovanillic acids in plasma using capillary gas chromatography-mass spectrometry and stable isotope dilution: preliminary data. Chromatographia 1993; 36: 179–82. 10.1007/BF02263858Search in Google Scholar
14 Maurer HH, Kraemer T, Weber A. Toxicological detection of ibuprofen and its metabolites in urine using gas chromatography-mass spectrometry (GC-MS). Pharmazie 1994; 49: 148–50. Search in Google Scholar
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- Quality control for SELDI analysis
- Immunobead multiplex RT-PCR detection of carcinoembryonic genes expressing cells in the blood of colorectal cancer patients
- Optimization and evaluation of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) with reversed-phase protein arrays for protein profiling
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