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The Measurement of Insulin-like Growth Factor-I (IGF-1) Concentration in Random Urine Samples

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Published/Copyright: June 1, 2005
Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Volume 40 Issue 6

Abstract

The aim of the present study was to assess a suitable expression of the urinary concentration of a protein/peptide hormone such as insulin-like growth factor-I (IGF-I), measured in the urine of healthy individuals when the specimen collection is executed randomly. One hundred and twenty male subjects were divided by age into four groups, namely healthy sedentary young (SYA) and older (SOA) adults, older (OC) and young (YC) children. In a single urine specimen, randomly collected during the morning from each individual, total urinary IGF-I was measured by immunoradiometric method, and urinary creatinine (uCr) and total proteins (utPr) were measured by capillary electrophoresis and spectrophotometric methods, respectively. The urinary IGF-I concentrations were not significantly different in all groups investigated and they were (mean±SD): 82.7±82.8 ng/l, 103.5±83.3 ng/l, 80.4±64.4 ng/l in OC, SYA and SOA, respectively; only in the YC group there was a tendency to higher values (125.2±93.2 ng/l) compared with the other groups. utPr ranged from 26 to 40 mg/l and did not demonstrate significant differences between groups. The urinary IGF-I correlated with uCr and utPr, and statistical significance was observed in all measurements. The measurement of urinary IGF-I in random urine and its ratio to utPr is an innovative, useful way of investigation of urinary protein/peptide hormones.

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Published Online: 2005-06-01
Published in Print: 2002-06-21

Copyright © 2002 by Walter de Gruyter GmbH & Co. KG

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  20. Meetings and Awards
https://doi.org/10.1515/CCLM.2002.099

Articles in the same Issue

  1. IFCC Primary Reference Procedures for the Measurement of Catalytic Concentrations of Enzymes – the New Concept
  2. Allele Frequency of the G→T Mutation of the Col1A1 Gene Analyzed by an ARMS-PCR in Osteoporotic Subjects with Femoral Neck Fractures
  3. Vitamin Supplementation Normalizes Total Plasma Homocysteine Concentration but Not Plasma Homocysteine Redox Status in Patients with Acute Coronary Syndromes and Hyperhomocysteinemia
  4. Mutation Analysis of the MPZ and PMP22 Genes in Croatian Patients
  5. Dynamic Equilibrium between Protein S and C4b Binding Protein Is Important for Accurate Determination of Free Protein S Antigen
  6. Immunoassay of Anti-Thyroid Autoantibodies: High Analytical Variability in Second Generation Methods
  7. The Measurement of Insulin-like Growth Factor-I (IGF-1) Concentration in Random Urine Samples
  8. Urinary Bikunin Determination Provides Insight into Proteinase/Proteinase Inhibitor Imbalance in Patients with Inflammatory Diseases
  9. Breath Markers and Soluble Lipid Peroxidation Markers in Critically Ill Patients
  10. Comparison of Laser-Probe and Photometric Determination of the Urinary Crystallization Risk of Calcium Oxalate
  11. Comparison of First Trimester, Second Trimester and Integrated Downs Syndrome Screening Results in Unaffected Pregnancies
  12. Anti-Phospholipid Antibodies in Diabetes Mellitus
  13. Alteration of Serum Enzymes in Primary Hypothyroidism
  14. The Changes of Superoxide Dismutase, Catalase and Glutathione Peroxidase Activities in Erythrocytes of Active and Passive Smokers
  15. Internal Quality Control System for Non-Stationary, Non-Ergodic Analytical Processes Based upon Exponentially Weighted Estimation of Process Means and Process Standard Deviation
  16. Evaluation of Commutability of Control Materials
  17. IFCC Primary Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes at 37°C. Part 1. The Concept of Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes
  18. IFCC Primary Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes at 37°C. Part 2. Reference Procedure for the Measurement of Catalytic Concentration of Creatine Kinase
  19. IFCC Primary Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes at 37°C. Part 3. Reference Procedure for the Measurement of Catalytic Concentration of Lactate Dehydrogenase
  20. Meetings and Awards
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